Biosynthesis and Functions of Very-Long-Chain Fatty Acids in the Responses of Plants to Abiotic and Biotic Stresses

Batsale, Marguerite; Bahammou, Delphine; Fouillen, Laëtitia; Mongrand, Sébastien; Joubès, Jérôme; Domergue, Frédéric

doi:10.3390/cells10061284

Cited by 118 publications

(92 citation statements)

References 161 publications

(273 reference statements)

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“…According to the studies in yeast, sphingolipids could act as signaling molecules functioning in the physiological adaptations to heat stress in plants. In higher plants, how sphingolipids affect heat stress signaling and thermotolerance has remained unexplored ( Markham et al, 2013 ; Batsale et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Lipidomic Remodeling in Begonia grandis Under Heat Stress

et al. 2022

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Characterization of the alterations in leaf lipidome in Begonia (Begonia grandis Dry subsp. sinensis) under heat stress will aid in understanding the mechanisms of stress adaptation to high-temperature stress often occurring during hot seasons at southern areas in China. The comparative lipidomic analysis was performed using leaves taken from Begonia plants exposed to ambient temperature or heat stress. The amounts of total lipids and major lipid classes, including monoacylglycerol (MG), diacylglycerol (DG), triacylglycerols (TG), and ethanolamine-, choline-, serine-, inositol glycerophospholipids (PE, PC, PS, PI) and the variations in the content of lipid molecular species, were analyzed and identified by tandem high-resolution mass spectrometry. Upon exposure to heat stress, a substantial increase in three different types of TG, including 18:0/16:0/16:0, 16:0/16:0/18:1, and 18:3/18:3/18:3, was detected, which marked the first stage of adaptation processes. Notably, the reduced accumulation of some phospholipids, including PI, PC, and phosphatidylglycerol (PG) was accompanied by an increased accumulation of PS, PE, and phosphatidic acid (PA) under heat stress. In contrast to the significant increase in the abundance of TG, all of the detected lysophospholipids and sphingolipids were dramatically reduced in the Begonia leaves exposed to heat stress, suggesting that a very dynamic and specified lipid remodeling process is highly coordinated and synchronized in adaptation to heat stress in Begonia plants.

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Section: Resultsmentioning

confidence: 99%

Lipidomic Remodeling in Begonia grandis Under Heat Stress

et al. 2022

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“…However, a LACS‐mediated plastid fatty acid export and fatty acyl‐activation by outer plastidial envelope localised LACS9 (Schnurr et al ., 2002) remain a possibility. The acyl‐CoA intermediates are elongated to very‐long‐chain fatty acids (VLCFA) (> C18) by the ER membrane‐embedded fatty acid elongase (FAE) complex in which ketoacyl‐CoA synthases (KCS) catalyse the rate‐limiting step (Haslam & Kunst, 2013; Batsale et al ., 2021) and are involved in producing suberin VLCFA‐CoA products of different chain lengths (KCS2, KCS20, KCS6 and probably KCS1) (Todd et al ., 1999; Franke et al ., 2009; Lee et al ., 2009; Serra et al ., 2009a). To produce primary alcohols, fatty acyl reductases (FAR) reduce the α‐carboxylic group of (L)VLCFA‐CoA intermediates: FAR1, FAR4 and FAR5 are partially overlapping chain‐length‐specific FARs that produce the suberin primary alcohols (Domergue et al ., 2010).…”

Section: Suberin Monomeric Precursor Formation and Transportmentioning

confidence: 99%

The making of suberin

Serra

Geldner

2022

New Phytologist

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Summary Outer protective barriers of animals use a variety of bio‐polymers, based on either proteins (e.g. collagens), or modified sugars (e.g. chitin). Plants, however, have come up with a particular solution, based on the polymerisation of lipid‐like precursors, giving rise to cutin and suberin. Suberin is a structural lipophilic polyester of fatty acids, glycerol and some aromatics found in cell walls of phellem, endodermis, exodermis, wound tissues, abscission zones, bundle sheath and other tissues. It deposits as a hydrophobic layer between the (ligno)cellulosic primary cell wall and plasma membrane. Suberin is highly protective against biotic and abiotic stresses, shows great developmental plasticity and its chemically recalcitrant nature might assist the sequestration of atmospheric carbon by plants. The aim of this review is to integrate the rapidly accelerating genetic and cell biological discoveries of recent years with the important chemical and structural contributions obtained from very diverse organisms and tissue layers. We critically discuss the order and localisation of the enzymatic machinery synthesising the presumed substrates for export and apoplastic polymerisation. We attempt to explain observed suberin linkages by diverse enzyme activities and discuss the spatiotemporal relationship of suberin with lignin and ferulates, necessary to produce a functional suberised cell wall.

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“…The regulatory mechanisms can be grouped into key factors such as transcriptional and post‐translational factors. Metabolites, hormones, long noncoding RNAs (lncRNA), miRNAs, and stress are the key interactive factors that modulate TAG formation (Batsale et al, 2021 ; Coleman & Lee, 2004 ). In addition, LD biogenesis is also regulated dynamically through the LD‐associated proteins (Chapman et al, 2019 ; Gidda et al, 2016 ; Pagac et al, 2016 ; Shi et al, 2013 ).…”

Section: Regulation Of Tag Biosynthesismentioning

confidence: 99%

Can omic tools help generate alternative newer sources of edible seed oil?

2022

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There are three pathways for triacylglycerol (TAG) biosynthesis: De novo TAG biosynthesis, phosphatidylcholine-derived biosynthesis, and cytosolic TAG biosynthesis.Variability in fatty acid composition is mainly associated with phosphatidylcholinederived TAG pathway. Mobilization of TAG-formed through cytosolic pathway into lipid droplets is yet unknown. There are multiple regulatory checkpoints starting from acetyl-CoA carboxylase to the lipid droplet biogenesis in TAG biosynthesis. Although a primary metabolism, only a few species synthesize oil in seeds for storage, and less than 10 species are commercially exploited. To meet out the growing demand for oil, diversifying into newer sources is the only choice left. The present review highlights the potential strategies targeting species like Azadirachta, Callophyllum, Madhuca, Moringa, Pongamia, Ricinus, and Simarouba, which are not being used for eating but are otherwise high yielding (ranging from 1.5 to 20 tons per hectare) with seeds having a high oil content (40-60%). Additionally, understanding the toxin biosynthesis in Ricinus and Simarouba would be useful in developing toxin-free oil plants. Realization of the importance of cell cultures as "oil factories" is not too far into the future and would soon be a commercially viable option for producing oils in vitro, round the clock. Highlight• Newer alternative sources like cell cultures and high yielding (nonedible) species with high oil content are commercially viable targets to engineer in producing edible-grade oil using the omics tool.

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Biosynthesis and Functions of Very-Long-Chain Fatty Acids in the Responses of Plants to Abiotic and Biotic Stresses

Cited by 118 publications

References 161 publications

Lipidomic Remodeling in Begonia grandis Under Heat Stress

Lipidomic Remodeling in Begonia grandis Under Heat Stress

The making of suberin

Can omic tools help generate alternative newer sources of edible seed oil?

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