Biosynthesis and metabolism of abscisic acid in tomato leaves infected withBotrytis cinerea

Kettner, Jens; Dörffling, Karl

doi:10.1007/bf01106753

Cited by 102 publications

(12 citation statements)

References 28 publications

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“…In the years 1970–1980, chemical studies revealed that some fungi were able to produce ABA which was until then considered as a hormone specifically produced by plants (Marumo et al ., ; Hirai et al ., ; Kettner and Dörffling, ; Assante et al ., ; Neill et al ., ). The well‐known ABA‐producing fungi are the polyphagous species B. cinerea (Leotiomycetes; Sclerotiniaceae) and the three species of the distantly related Cercospora (Dothideomycetes; Mycosphaerellaceae), i.e., C. rosicola , C. cruenta and C. pini‐densiflora , that are respectively pathogenic towards rose, cowpea and Japanese red pine.…”

Section: Discussionmentioning

confidence: 98%

“…Nevertheless, several screening and mutagenesis programs were conducted to obtain industrial strains that produce high levels of ABA for agronomic uses. The resulting improved strains such as ATCC58025 are impaired in conidiation, sclerotia formation and pathogenicity but produce up to 6 g l −1 of ABA in fermenters (Kettner and Dörffling, ;Siewers et al ., ; Ding et al ., ; Shi et al ., ). By investigating the P450 monooxygenase‐coding genes that are highly expressed during the production of ABA in the strain ATCC58025, Siewers and colleagues () identified a first gene ( Bcaba1 ) involved in the biosynthesis of this sesquiterpene.…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of abscisic acid in fungi: identification of a sesquiterpene cyclase as the key enzyme in Botrytis cinerea

Izquierdo-Bueno

González‐Rodríguez

Simon

et al. 2018

Environmental Microbiology

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While abscisic acid (ABA) is known as a hormone produced by plants through the carotenoid pathway, a small number of phytopathogenic fungi are also able to produce this sesquiterpene but they use a distinct pathway that starts with the cyclization of farnesyl diphosphate (FPP) into 2Z,4E-α-ionylideneethane which is then subjected to several oxidation steps. To identify the sesquiterpene cyclase (STC) responsible for the biosynthesis of ABA in fungi, we conducted a genomic approach in Botrytis cinerea. The genome of the ABA-overproducing strain ATCC58025 was fully sequenced and five STC-coding genes were identified. Among them, Bcstc5 exhibits an expression profile concomitant with ABA production. Gene inactivation, complementation and chemical analysis demonstrated that BcStc5/BcAba5 is the key enzyme responsible for the key step of ABA biosynthesis in fungi. Unlike what is observed for most of the fungal secondary metabolism genes, the key enzyme-coding gene Bcstc5/Bcaba5 is not clustered with the other biosynthetic genes, i.e., Bcaba1 to Bcaba4 that are responsible for the oxidative transformation of 2Z,4E-α-ionylideneethane. Finally, our study revealed that the presence of the Bcaba genes among Botrytis species is rare and that the majority of them do not possess the ability to produce ABA.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Biosynthesis of abscisic acid in fungi: identification of a sesquiterpene cyclase as the key enzyme in Botrytis cinerea

Izquierdo-Bueno

González‐Rodríguez

Simon

et al. 2018

Environmental Microbiology

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“…vesicatoria-infected Arabidopsis was judged to be plant derived based on the absence of such genes in the reported genome (8). Similarly, the induction of ABA levels by fungal pathogens has been reported in tomato with a portion of the ABA being pathogen derived (48). In Pst-infected Arabidopsis, induced increases in ABA are small and likely a secondary response to biotic stress.…”

Section: Pst Infection Results In Cor Accumulation and Widespread Chamentioning

confidence: 99%

Simultaneous analysis of phytohormones, phytotoxins, and volatile organic compounds in plants

Schmelz

Engelberth

Alborn

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

302

215

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Phytohormones regulate the protective responses of plants against both biotic and abiotic stresses by means of synergistic or antagonistic actions referred to as signaling crosstalk. A bottleneck in crosstalk research is the quantification of numerous interacting phytohormones and regulators. The chemical analysis of salicylic acid, jasmonic acid, indole-3-acetic acid, and abscisic acid is typically achieved by using separate and complex methodologies. Moreover, pathogen-produced phytohormone mimics, such as the phytotoxin coronatine (COR), have not been directly quantified in plant tissues. We address these problems by using a simple preparation and a GC-MS-based metabolic profiling approach. Plant tissue is extracted in aqueous 1-propanol and mixed with dichloromethane. Carboxylic acids present in the organic layer are methylated by using trimethylsilyldiazomethane; analytes are volatilized under heat, collected on a polymeric absorbent, and eluted with solvent into a sample vial. Analytes are separated by using gas chromatography and quantified by using chemical-ionization mass spectrometry that produces predominantly [M؉H] ؉ parent ions. We use this technique to examine levels of COR, phytohormones, and volatile organic compounds in model systems, including Arabidopsis thaliana during infection with Pseudomonas syringae pv. tomato DC3000, corn (Zea mays) under herbivory by corn earworm (Helicoverpa zea), tobacco (Nicotiana tabacum) after mechanical damage, and tomato (Lycopersicon esculentum) during drought stress. Numerous complex changes induced by pathogen infection, including the accumulation of COR, salicylic acid, jasmonic acid, indole-3-acetic acid, and abscisic acid illustrate the potential and simplicity of this approach in quantifying signaling crosstalk interactions that occur at the level of synthesis and accumulation.

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“…The above ground plants were harvested from each plot, dried at 70 • C for 48 h, ground at the grinding mill and samples were stored in Ziploc polyethylene bags at room temperature till nutrient analysis. Total phosphorus in plant samples and in seeds was analyzed through Olsen and Sommers [30]. However, phosphorus concentration in shoot was used for the calculation of plant P uptake (kg ha −1 ).…”

Section: Yield Physiology and Plant Nutrient Analysismentioning

confidence: 99%

Rock Phosphate-Enriched Compost in Combination with Rhizobacteria; A Cost-Effective Source for Better Soil Health and Wheat (Triticum aestivum) Productivity

et al. 2020

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Organic materials from various sources have been commonly adopted as soil amendments to improve crop productivity. Phosphorus deficiency and fixation in alkaline calcareous soils drives a reduction in crop production. A two-year field experiment was conducted to evaluate the impact of rock phosphate enriched composts and chemical fertilizers both individually and in combination with plant growth promoting rhizobacteria (PGPR) on wheat productivity and soil chemical and biological and biochemical properties. The present study demonstrates significant increments in crop agronomic and physiological parameters with Pseudomonas sp. inoculated RPEC1 (rock phosphate + poultry litter + Pseudomonas sp.) over the un-inoculated untreated control. However, among all other treatments i.e., RPEC2 (rock phosphate + poultry litter solubilized with Proteus sp.), RPC (rock phosphate + poultry litter), HDP (half dose inorganic P from Single Super Phosphate-SSP 18% P2O5) and SPLC (poultry litter only); RPEC1 remained the best by showing increases in soil chemical properties (available phosphorus, nitrate nitrogen, extractable potassium), biochemical properties (alkaline phosphatase activity) and biological properties (microbial biomass carbon and microbial biomass phosphorus). Economic analysis in terms of Value Cost Ratio (VCR) showed that the seed inoculation with Pseudomonas sp. in combination with RPEC1 gave maximum VCR (3.23:1) followed by RPEC2 (2.61:1), FDP (2.37:1), HDP (2.05:1) and SPLC (2.03:1). It is concluded that inoculated rock phosphate (RP) enriched compost (RPEC1) can be a substitute to costly chemical fertilizers and seed inoculation with Pseudomonas sp. may further increase the efficiency of composts.

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Biosynthesis and metabolism of abscisic acid in tomato leaves infected withBotrytis cinerea

Cited by 102 publications

References 28 publications

Biosynthesis of abscisic acid in fungi: identification of a sesquiterpene cyclase as the key enzyme in Botrytis cinerea

Biosynthesis of abscisic acid in fungi: identification of a sesquiterpene cyclase as the key enzyme in Botrytis cinerea

Simultaneous analysis of phytohormones, phytotoxins, and volatile organic compounds in plants

Rock Phosphate-Enriched Compost in Combination with Rhizobacteria; A Cost-Effective Source for Better Soil Health and Wheat (Triticum aestivum) Productivity

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