Biosynthesis and production of polysialic acids in bacteria

Ferrero, Miguel Ángel Martín; Rodrı́guez-Aparicio, Leandro B.

doi:10.1007/s00253-010-2531-5

Cited by 38 publications

(22 citation statements)

References 142 publications

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“…The E. coli K1 and K92 capsules use polysialic acid (PSA) as the nucleotide sugars (17,78,79). In the biosynthesis pathway of PSA capsule, NeuA (CMP-Neu5Ac synthetase) and NeuS are two signature enzymes.…”

Section: Discussionmentioning

confidence: 99%

Abrogation of Neuraminidase Reduces Biofilm Formation, Capsule Biosynthesis, and Virulence of Porphyromonas gingivalis

Kurniyati

Hu³

et al. 2012

Infect Immun

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The oral bacterium Porphyromonas gingivalis is a key etiological agent of human periodontitis, a prevalent chronic disease that affects up to 80% of the adult population worldwide. P. gingivalis exhibits neuraminidase activity. However, the enzyme responsible for this activity, its biochemical features, and its role in the physiology and virulence of P. gingivalis remain elusive. In this report, we found that P. gingivalis encodes a neuraminidase, PG0352 (Sia Pg ). Transcriptional analysis showed that PG0352 is monocistronic and is regulated by a sigma 70 -like promoter. Biochemical analyses demonstrated that Sia Pg is an exo-␣-neuraminidase that cleaves glycosidic-linked sialic acids. Cryoelectron microscopy and tomography analyses revealed that the PG0352 deletion mutant (⌬PG352) failed to produce an intact capsule layer. Compared to the wild type, in vitro studies showed that ⌬PG352 formed less biofilm and was less resistant to killing by the host complement. In vivo studies showed that while the wild type caused a spreading type of infection that affected multiple organs and all infected mice were killed, ⌬PG352 only caused localized infection and all animals survived. Taken together, these results demonstrate that Sia Pg is an important virulence factor that contributes to the biofilm formation, capsule biosynthesis, and pathogenicity of P. gingivalis, and it can potentially serve as a new target for developing therapeutic agents against P. gingivalis infection.

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Section: Discussionmentioning

confidence: 99%

Abrogation of Neuraminidase Reduces Biofilm Formation, Capsule Biosynthesis, and Virulence of Porphyromonas gingivalis

Kurniyati

Hu³

et al. 2012

Infect Immun

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“…In bacteria three PSA variants are produced as capsular polysaccharides (for a review of sialic acid biosynthesis see [8]). Neuro-invasive human pathogens Escherichia coli K1 and N. meningitidis group B as well as the bovine/ovine pathogen Mannheimia haemolytica A2 produce a capsule composed of homopolymer of α2,8-linked Neu5Ac residues [9], [10].…”

Section: Introductionmentioning

confidence: 99%

Biochemical Characterization of a Polysialyltransferase from Mannheimia haemolytica A2 and Comparison to Other Bacterial Polysialyltransferases

et al. 2013

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Polysialic acids are bioactive carbohydrates found in eukaryotes and some bacterial pathogens. The bacterial polysialyltransferases (PSTs), which catalyze the synthesis of polysialic acid capsules, have previously been identified in select strains of Escherichia coli and Neisseria meningitidis and are classified in the Carbohydrate-Active enZYmes Database as glycosyltransferase family GT-38. In this study using DNA sequence analysis and functional characterization we have identified a novel polysialyltransferase from the bovine/ovine pathogen Mannheimia haemolytica A2 (PSTMh). The enzyme was expressed in recombinant form as a soluble maltose-binding-protein fusion in parallel with the related PSTs from E. coli K1 and N. meningitidis group B in order to perform a side-by-side comparison. Biochemical properties including solubility, acceptor preference, reaction pH optima, thermostability, kinetics, and product chain length for the enzymes were compared using a synthetic fluorescent acceptor molecule. PSTMh exhibited biochemical properties that make it an attractive candidate for chemi-enzymatic synthesis applications of polysialic acid. The activity of PSTMh was examined on a model glycoprotein and the surface of a neuroprogenitor cell line where the results supported its development for use in applications to therapeutic protein modification and cell surface glycan remodelling to enable cell migration at implantation sites to promote wound healing. The three PSTs examined here demonstrated different properties that would each be useful to therapeutic applications.

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“…Mannosamine, i.e. N-acetyl D-mannosamine containing sialic acids are present as protective capsular components of bacteria, invading mammals (Ferrero and Aparicio 2010). However, small amounts of mannosamine may be also present as common linkage units between peptidoglycan and other bacterial cell wall components such as glycerol teichoic acid (Yoneyama et al 1982).…”

Section: Introductionmentioning

confidence: 99%

Optimisation of amino sugar quantification by HPLC in soil and plant hydrolysates

et al. 2011

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Amino sugars are increasingly used as indicators for the accumulation of microbial residues in soil and plant material. A reverse-phase high-performance liquid chromatography method was improved for the simultaneous determination of muramic acid, mannosamine, glucosamine and galactosamine in soil and plant hydrolysates via orthophthaldialdehyde (OPA) pre-column derivatisation and fluorescence detection. The retention time was reduced, and the separation of muramic acid and mannosamine was optimised by modifying the mobile phase. The effects of excitation wavelength, OPA reaction time, tetrahydrofuran concentration and pH value of the mobile phase on the amino sugar separation were tested. Quantification limits were in the range of 0.13 to 0.90 μg ml −1 . No interferences exist from amino acids or other primary amines, occurring in soil and plant hydrolysates.

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Biosynthesis and production of polysialic acids in bacteria

Cited by 38 publications

References 142 publications

Abrogation of Neuraminidase Reduces Biofilm Formation, Capsule Biosynthesis, and Virulence of Porphyromonas gingivalis

Abrogation of Neuraminidase Reduces Biofilm Formation, Capsule Biosynthesis, and Virulence of Porphyromonas gingivalis

Biochemical Characterization of a Polysialyltransferase from Mannheimia haemolytica A2 and Comparison to Other Bacterial Polysialyltransferases

Optimisation of amino sugar quantification by HPLC in soil and plant hydrolysates

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