Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii

Gorbunov, Aleksandr A; Akentyev, Philipp; Gubaidullin, I. I.; Zhiganov, N. I.; Tereshchenkova, Valeriia F.; Elpidina, Elena N.; Козлов, Д. Г.

doi:10.1134/s0003683821090039

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Most of them are in the "S" group, many of which act in digestion (Bao et al, 2014;Cao & Jiang, 2018;Prabhakar et al, 2007;Wolfson et al, 1990). Additionally, they may possess a prominent role in rapid dietary adaptations (Gorbunov et al, 2021;Kumar et al, 2021). Similarly, transcripts of 14 SPs/SPHs are most abundant in salivary glands, where they may also act in digestion (Allen & Walker, 2012;Amino et al, 2001;Walker et al, 2018).…”

Section: Expression Profiling Of Sp/sph Genesmentioning

confidence: 99%

Identification and expression profiling of serine protease‐related genes in Tenebrio molitor

Xiao

Wang

et al. 2022

Arch Insect Biochem Physiol

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In insects, serine proteases and serine protease homologs (SPs/SPHs) are involved in a variety of physiological processes including digestion, development, and immunity.Here, we identified 112 SP and 88 SPH genes in the genome of the yellow mealworm, Tenebrio molitor. Based on the features of domain structure, they were divided into "S" group containing single Tryp-SPc or Tryp-SPHc domain, "C" group containing 1-4 CLIP domain (CLIPA-D) and "M"

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Section: Expression Profiling Of Sp/sph Genesmentioning

confidence: 99%

Identification and expression profiling of serine protease‐related genes in Tenebrio molitor

Xiao

Wang

et al. 2022

Arch Insect Biochem Physiol

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“…Analyzing trypsin-like SPs/SPHs in transcriptome datasets from different stages of the T. molitor life cycle, we have previously de novo assembled 54 trypsins and five trypsin-like SPHs [ 31 ]. We also characterized recombinant preparations of SP, SerP38, and SPH, SerPH122, expressed in the Komagataella kurtzmanii system [ 32 , 33 ]. Recent work by Wu and coauthors [ 34 ] provided information on 200 T. molitor genes including 112 SPs and 88 SPHs, and transcriptome datasets together with RT-PCR analysis were used for SP-related genes expression profiling at various developmental stages and tissues.…”

Section: Introductionmentioning

confidence: 99%

The Set of Serine Peptidases of the Tenebrio molitor Beetle: Transcriptomic Analysis on Different Developmental Stages

Zhiganov,

Vinokurov,

Salimgareev

et al. 2024

IJMS

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Serine peptidases (SPs) of the chymotrypsin S1A subfamily are an extensive group of enzymes found in all animal organisms, including insects. Here, we provide analysis of SPs in the yellow mealworm Tenebrio molitor transcriptomes and genomes datasets and profile their expression patterns at various stages of ontogeny. A total of 269 SPs were identified, including 137 with conserved catalytic triad residues, while 125 others lacking conservation were proposed as non-active serine peptidase homologs (SPHs). Seven deduced sequences exhibit a complex domain organization with two or three peptidase units (domains), predicted both as active or non-active. The largest group of 84 SPs and 102 SPHs had no regulatory domains in the propeptide, and the majority of them were expressed only in the feeding life stages, larvae and adults, presumably playing an important role in digestion. The remaining 53 SPs and 23 SPHs had different regulatory domains, showed constitutive or upregulated expression at eggs or/and pupae stages, participating in regulation of various physiological processes. The majority of polypeptidases were mainly expressed at the pupal and adult stages. The data obtained expand our knowledge on SPs/SPHs and provide the basis for further studies of the functions of proteins from the S1A subfamily in T. molitor.

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“…This strain was also registered in CBS under the number CBS 12817 and included in the collection of Komagataella species selected for genotypic and phenotypic diversity analysis (Heistinger et al, 2022). A competitive recombinant expression system was developed and successfully applied based on K. kurtzmanii VKPM Y‐727 (Gorbunov et al, 2021; Gubaidullin et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Expression level of SOR1 is a bottleneck for efficient sorbitol utilization by yeast Komagataella kurtzmanii

Akentyev

Sokolova

Korzhenkov

et al. 2023

Yeast

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The yeast strain Komagataella kurtzmanii VKPM Y‐727 shows a significant defect in sorbitol utilization compared to closely related yeast K. phaffii (including strains formerly identified as Pichia pastoris). Our aim was to investigate the factors that determine the phenotype of the wild‐type strain and to obtain a K. kurtzmanii strain with an improved ability to utilize sorbitol. We sequenced and annotated the genome of K. kurtzmanii VKPM Y‐727 and compared it with that of K. phaffii GS115. Five K. phaffii GS115 genes that might be involved in sorbitol metabolism were selected and transferred into K. kurtzmanii Y‐727. The transfer of the modified SOR1 gene resulted in an increased growth rate of K. kurtzmanii in sorbitol, despite the fact that Y‐727 already contains its own SOR1 gene without any apparent mutations. The enzymes encoded by the SOR1 genes were analyzed in vitro and found to have similar properties. Differences in promoter activity were assessed using lacZ as a reporter gene, and the PSDH727 (promoter of SOR1 (SDH727) from K. kurtzmanii Y‐727) promoter was shown to be 1.5–2.0 times weaker than PSDH115 (promoter of SOR1 (SDH115) from K. phaffii GS115). Moreover, both promoters were less active in K. kurtzmanii than in K. phaffii when evaluated in cells grown in synthetic complete media with glucose or sorbitol. Thus, SOR1 gene expression was identified as a bottleneck in sorbitol metabolism in K. kurtzmanii. Also, the positive effect of additional modified SOR1 gene copies was observed in both yeasts, as K. kurtzmanii and K. phaffii could grow on synthetic complete media with sorbitol three times faster than the original K. phaffii GS115 strain.

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Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii

Cited by 4 publications

References 15 publications

Identification and expression profiling of serine protease‐related genes in Tenebrio molitor

Identification and expression profiling of serine protease‐related genes in Tenebrio molitor

The Set of Serine Peptidases of the Tenebrio molitor Beetle: Transcriptomic Analysis on Different Developmental Stages

Expression level of SOR1 is a bottleneck for efficient sorbitol utilization by yeast Komagataella kurtzmanii

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