Biosynthesis of Bacitracin in Stirred Fermenter by Bacillus Licheniformis Using Defatted Oil Seed Cakes as Substrate

Zarei, Iman

doi:10.5539/mas.v6n2p30

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…In our study, the optimum temperature for antimicrobial compound production was observed to be 30 to 35 uC. Béahdy (1974) and Haddar et al (2007) observed production of bacitracin and other antibiotics by B. licheniformis (Zarei, 2012) at 37 uC, and it was also seen at 30 uC by Hosny et al (2011).…”

Section: Microbiologysupporting

confidence: 64%

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Zada¹,

Naseem²,

Lee³

et al. 2016

JCKS

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Bacterial strains having the ability to inhibit the growth of other bacteria were isolated from soil samples collected from Kashmir Smast (smast is Pushto for cave), Khyber Pakhtunkhwa, Pakistan. The study includes mineralogical and geochemical analyses of soil sample collected from the cave, so as to describe the habitat from which the microorganisms have been isolated. Total bacterial count of the soil sample was 5.25 3 10 4 CFU mL −1. Four bacterial isolates having activity against test organisms Micrococcus luteus, Klebsiella sp., Pseudomonas sp., and Staphylococcus aureus were screened out for further study. Two of the isolates were found to be Gram-positive and the other two Gram-negative. The four isolates showing antibacterial activity were identified as Serratia sp. KC1-MRL, Bacillus licheniformis KC2-MRL, Bacillus sp. KC3-MRL, and Stenotrophomonas sp. KC4-MRL on the basis of 16S rRNA sequence analysis. Although all isolates showed antibacterial activity, only Bacillus licheniformis KC2-MRL was selected for further study due to its large zone of inhibition. Antibacterial activity of B. licheniformis KC2-MRL was optimum when grown in nutrient broth adjusted to pH 5 and after 24 hours of incubation at 35 uC. The extracted antibacterial compound was stable at pH 5-7 and 40 uC when incubated for 1 hour. The strain was found resistant against cefotaxime (ctx). Atomic-absorption analysis of the soil sample collected from the cave showed high concentrations of calcium (332.938 mg kg ) compared to the control soil collected outside the cave. FTIR spectrum of the concentrated protein showed similarity to bacitracin. The antibacterial compound showed activity against both Gram-negative and positive test strains. Mineralogy of Kashmir Smast is diverse and noteworthy. Different geochemical classes identified by X-ray diffraction were nitrates, oxides, phosphates, silicates, and sulfates. Weathered cave limestone contributes notably to the formation of these minerals or compounds. FTIR spectroscopic analysis helped to identify minerals such as quartz, clinochlore, vermiculite, illite, calcite, and biotite.

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Section: Microbiologysupporting

confidence: 64%

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Zada¹,

Naseem²,

Lee³

et al. 2016

JCKS

View full text Add to dashboard Cite

show abstract

“…Sunflower, soybean, and sesame meal were used for the production of clavulanic acid and cephamycin C and by SSF with Bacillus licheniformis for endotoxin and bacitracin. The maximum synthesis of the latter was found in soybean meal [22,[160][161][162].…”

Section: Antibioticsmentioning

confidence: 99%

Oil Press-Cakes and Meals Valorization through Circular Economy Approaches: A Review

2020

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The food industry generates a large amount of waste every year, which opens up a research field aimed at minimizing and efficiently managing this issue to support the concept of zero waste. From the extraction process of oilseeds results oil cakes. These residues are a source of bioactive compounds (protein, dietary fiber, antioxidants) with beneficial properties for health, that can be used in foods, cosmetics, textile, and pharmaceutical industries. They can also serve as substrates for the production of enzymes, antibiotics, biosurfactants, and mushrooms. Other applications are in animal feedstuff and for composites, bio-fuel, and films production. This review discusses the importance of oilseed and possible valorization methods for the residues obtained in the oil industry.

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Optimization of enhanced microbial production of zinc bacitracin by submerged fermentation technology

et al. 2020

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Bacitracin is one of the most important antibiotics used in different biomedical fields. It helps to achieve sizeable amount of foreign exchange due to its use in the poultry feed. The cheap agricultural wastes are readily available for the preparation of fermentation media used for bacitracin production. The microorganisms could be mutated with different chemicals and UV radiation to improve bacitracin production. Thus, the current study was focused on the synthesis of low‐cost and effective bacitracin by mutant strains of Bacillus licheniformis, employing the submerged fermentation technique. The bacteria were exposed to the UV irradiation for various time periods ranging from 5 to 40 min. These mutants were named as BLAA‐5–BLAA‐40. Mutant strain BLAA‐25 produced maximum bacitracin, with significantly high activity (142.81 IU/mg) against Klebsiella pneumoniae but less activity against Escherichia coli (115.19 IU/mg). Several fermentation conditions were investigated to optimize bacitracin production. The highest bacitracin yield was obtained by an inoculum size of 10%, fermentation period 48 hr, pH 7, T = 37°C, using soybean meal as a substrate. Among all substrates, cucumber peel was the substrate showing the highest minimum inhibitory concentration (2.3 mg/ml and 2.7 mg/ml against K. pneumoniae and E. coli respectively). A comparison between commercial and experimentally produced Zn bacitracin showed that commercial bacitracin has a low activity (63.2 IU/mg) as compared with experimental bacitracin. Hence, the agro wastes and mutation could be used to increase the synthesis of Zn bacitracin in B. licheniformis.

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Biosynthesis of Bacitracin in Stirred Fermenter by Bacillus Licheniformis Using Defatted Oil Seed Cakes as Substrate

Cited by 6 publications

References 28 publications

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Oil Press-Cakes and Meals Valorization through Circular Economy Approaches: A Review

Optimization of enhanced microbial production of zinc bacitracin by submerged fermentation technology

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