Biosynthesis of dextrans with different molecular weights by selecting the concentration of Leuconostoc mesenteroides B-512FMC dextransucrase, the sucrose concentration, and the temperature

Enzymatic Transformations Involved in the Biosynthesis of Microbial Exo‐polysaccharides Based on the Assembly of Repeat Units

“…For this reason,t his minireview will focus on real (released) exo-polysaccharides (EPS). Likewise, examples of extracellularly synthesised polysaccharides (e.g.,d extran), whicha re described elsewhere, [1][2][3][4] are not the focus here.…”

Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose

“…15,18 Other factors influencing product properties, including temperature, enzyme concentration and extent of reaction, have also been investigated. 11,16 Dextrans of different molar mass were synthesized at controlled sucrose concentration, enzyme concentration and reaction temperature by dextransucrase from L. mesenteroides B-512FMC. 11 The glucansucrase GTFA of the probiotic bacterium Lactobacillus reuteri 121 catalyzes the synthesis of the exopolysaccharide (EPS) reuteran from sucrose.…”

“…11,16 Dextrans of different molar mass were synthesized at controlled sucrose concentration, enzyme concentration and reaction temperature by dextransucrase from L. mesenteroides B-512FMC. 11 The glucansucrase GTFA of the probiotic bacterium Lactobacillus reuteri 121 catalyzes the synthesis of the exopolysaccharide (EPS) reuteran from sucrose. 13 The EPS consists of 58% (1→4) and 42% (1→6) linkages with no repeating units present.…”

“…15,18 The degree of Page 4 of 23 polymerization/molar mass of α-glucan products may also vary due to reaction conditions, especially the concentration of sucrose. 11,12,[14][15][16] Already decades ago the molar mass of the Leuconostoc mesenteroides strain B512-F dextransucrase α-glucan products was studied throughout the reaction process. 17 The effects of sucrose concentration on product size distribution have been evaluated, showing decreased molar mass of dextran with increasing sucrose concentrations.…”

“…These -glucans have broad applications in the food and pharmaceutical industries. [8][9][10] The determinants of product size distribution of glucansucrases have been extensively studied, [11][12][13][14][15][16][17][18] however, much remains unknown. The amino acid repeats located in the glucansucrase N-terminal variable region and C-terminal glucan-binding domain have been shown to play a role in product size distribution.…”