Biosynthesis of ethyl butyrate using immobilized lipase: a statistical approach

Rodríguez‐Nogales, José Manuel; Roura, Elena; Contreras, Elizabeth

doi:10.1016/j.procbio.2003.11.049

Cited by 70 publications

(43 citation statements)

References 12 publications

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“…The maximum conversion rate was 75% at 35 °C, showing a substantial reduction as temperature increased from 20 up to 80 °C. The results presented in our work were better than those obtained by Rodriguez et al(Rodriguez-Nogales et al,2005), since a conversion rate of 90% was observed for ethyl butyrate at 25 °C.…”

Section: Effect Of Temperaturecontrasting

confidence: 82%

“…The ethanol esterification with butyric acid in the presence of hexane and heptane is reported by several authors (Dias et al, 1991;Manjon et al,1991;Dave et al, 2005;Rodriguez-Nogales et al, 2005;Aragão et al, 2011). The best results were obtained by Manjon et al (1991) using R. miehei lipase immobilized in Celite as catalyst and hexane as solvent.…”

Section: Production Of Flavor Esters In the Presence Of Organic Solventsmentioning

confidence: 73%

“…Other authors studied the effect of the temperature in ethyl butyrate production catalyzed by Novozym® 435 (Rodriguez-Nogales et al, 2005). The maximum conversion rate was 75% at 35 °C, showing a substantial reduction as temperature increased from 20 up to 80 °C.…”

Section: Effect Of Temperaturementioning

confidence: 99%

“…The literature (Dias et al, 1991;Manjon et al, 1991;Dave et al, 2005, Rodriguez-Nogales et al, 2005Aragão et al, 2011, Grosso et al, 2012 reports the effect of several different immobilized lipases in the production of ethyl butyrate. For instance (Rodriguez-Nogales et al,2005),the reaction with butyric acid and ethanol catalyzed by CALBacrylic resin (Novozym 435, Novozymes, Denmark) in 10 mL of n-heptane, with a concentration of acid and alcohol of 0.04 mol/L and 0.52 mol/L, respectively, was carried out and75% of conversion was achieved in 96 hours.…”

Section: Reaction Time Dependencementioning

confidence: 99%

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Production of flavor esters catalyzed by lipase B from Candida antarctica immobilized on magnetic nanoparticles

Souza

Santos

Freire

et al. 2017

Braz. J. Chem. Eng.

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-Candida antarctica Lipase B (CALB) immobilized onto iron magnetic nanoparticles was evaluated as biocatalyst for the synthesis of flavor esters. Methyl and ethyl butyrate were synthesized by esterification of butyric acid with methanol and ethanol, respectively, in a medium containing solvent. The nanoparticles were produced by the co-precipitation method. The process parameters (type of solvent, temperature, substrate concentration, molar ratio, amount of biocatalyst, stirring speed and reaction time) were studied. The optimum conditions for both esters were achieved at 25 °C, 0.5 mol/L (methyl butyrate) and 0.4 mol/L (ethyl butyrate), molar ratio of 1:1, amount of biocatalyst: 10 mg, 150 rpm and 8 h of reaction, using heptane as solvent. Under those conditions, the maximum conversions of methyl butyrate and ethyl butyrate were higher than 90 %. The synthesis of flavor esters was also conducted by using Novozym® 435, a commercial catalyst, for comparison purposes.

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Section: Effect Of Temperaturecontrasting

confidence: 82%

Section: Production Of Flavor Esters In the Presence Of Organic Solventsmentioning

confidence: 73%

Section: Effect Of Temperaturementioning

confidence: 99%

Section: Reaction Time Dependencementioning

confidence: 99%

See 2 more Smart Citations

Production of flavor esters catalyzed by lipase B from Candida antarctica immobilized on magnetic nanoparticles

Souza

Santos

Freire

et al. 2017

Braz. J. Chem. Eng.

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“…Models evaluated were made by variance analyses (ANOVA) that was statistical analyses to authenticate the models based in the Gauss curve deviation. Process optimization was evaluated by the response surface methodology (Barros Neto et al, 1995 and2001;Biazus et al, 2005;Ferreira et al, 2007;Rodriguez-Nogales et al, 2005;Severo Junior et al, 2007b). …”

Section: Experimental Design and Statistical Analysismentioning

confidence: 99%

Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp

Lopes

Júnior

Souza

et al. 2009

Braz. arch. biol. technol.

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The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2 2 experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and concentrated in 10 fold the bromelain extract. SDS-PAGE electrophoresis showed that the ultra-filtrated had high purity and the bromelain from A. comosus pulp had a molecular weight of 24.5 kDa.

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