Biosynthesis of fatty acids from acetate in soybean suspension cultures

Abstract

Suspension cultures of finely divided soybean cells established from callus were incubated with sodium [1(14)C] acetate for periods up to 86 hr. Lipids and fatty acids were analyzed for radioactivity in samples harvested at logarithmic time periods. Incorporation of acetate into cell lipid was directly proportional to the logarithm of time up to 32 hr, after an initial lag of 4-6 hr. Most of the lipid radioactivity was found in the phospholipid fraction, and all common soybean fatty acids became labeled within 6 hr. The order of labeling and distribution of radioactivity with time were essentially the same as in tissues from intact growing plants. These results support the concept of sequential desaturation of oleic acid in the cells. It was concluded that valid studies of the biosynthesis of common lipids in the soybean can be carried out for extended periods of time by use of undifferentiated cells in suspension cultures.

TLC and 31P‐NMR Analysis of Low Polarity Phospholipids

“…Apart from NAPE, an unknown lipid identified as BPA was reported for developing soybeans and soybean suspension cultures [14]. The authors mentioned that chromatographic mobility of the latter lipid is lower than that of NAPE, which allows separation of these two lipids by preparative TLC.…”

“…3). BPA was reported for marine bacteria [13], developing soybeans [14], fish brain [15], and degenerating cultured hamster fibroblasts [16]. BPA was also tentatively identified in some fish flesh [17].…”

Cocobean Tissue Culture: Lipid Composition and Fatty Acid Metabolism

“…Plant cell cultures are valuable models of plant cell systems for the studying of lipid metabolism (Stearns and Morton, 1975;Stumpf and Weber, 1977;Wilson et al, 1978;MacCarthy and Stumpf, 1980a, b). It is necessary Table 1 ( 1 10 days post-pollination), ripe cocoa beans, cocoa calli (5 wkl, and to understand the pattern and mechanism of lipid synthesis before one can manipulate the conditions required to produce desirable lipids in cell culture.…”

“…In recent years, plant tissue cultures have been actively exploited for biochemical studies of plant cell metabolism, especially plant lipid metabolism (Tattrie and Veliky, 1973;Jones 1974;Stearns and Morton 1975;Radwan and Mangold 1976;Stumpf and Weber 1977;Wilson et al, 1978;Radwan et al, 1979;MacCarthy and Stumpf 1980a,b). The technique of plant cell cultures hold a great potential as a method for production of useful products in vitro.…”

The effect of hormones and chemical growth regulators on ear development and grain yield of nonprolific corn (Zea mays L.)

“…Stearn and Morton (1975) The initial incorporation of ^^C-acetate into the total fatty acids in roots of frost-hardy and less hardy alfalfa {nedicago sativa) culti vars under hardening conditions, was studied by Griener et al (1975).…”