Biosynthesis of fluorescent silver nanoparticles from Leea coccinea leaves and their antibacterial potentialities against Xanthomonas phaseoli pv phaseoli

Abstract

The synthesis of silver nanoparticles (SNP) from plants is a simple, fast and environmentally safe route. In the present study, the aqueous extract of fresh leaves from Leea coccinea L. was evaluated as a possible source of reducing and stabilizing agents to obtain SNP. The synthesized SNP were characterized by spectroscopic techniques such as UV–visible spectrophotometry and Fourier transform infrared spectroscopy (FTIR), scanning electron and confocal microscopies and the antimicrobial activity against Xanthomonas phaseoli pv. phaseoli was evaluated using agar diffusion methods. The results showed that the evaluated extract was promising for the green synthesis of the SNP, which was visually identified by the formation of a dark-brown complex and the presence of a peak of maximum absorption at 470 nm in a UV–VIS spectrum. FTIR spectrum of SNP showed main characteristic signals of aromatic compounds, carboxylic group among others confirmed by phytochemical screening that made evident the presence of flavonoids, phenols, leucoanthocyanidins, terpenes and steroids groups. Fluorescent SNP with high degree of agglomeration were observed by the microscopical technics used. A promising antibacterial activity of SNP was shown by a zone of microbial growth inhibition. These results suggested the need for going deeper in the physico-chemical characterization and kinetic studies, as well as the biological evaluations to make possible the use of this plant source in the future development of antibacterial formulations for bean seed protection.

Long-Lasting Silver Nanoparticles Synthesized with Tagetes erecta and Their Antibacterial Activity against Erwinia amylovora, a Serious Rosaceous Pathogen

“…The antibacterial activity of the AgNPs synthesized with 1 mL of the aqueous extract of the T. erecta leaves against E. amylovora was evaluated using the standard Kirby-Bauer disk diffusion method [22,[71][72][73]. Sterile paper discs (Whatman No.…”

Green Synthesis and Characterization of Silver Nanoparticles from Thymus Vulgaris Leaf Extract

“…All the characterization studies were done in SAIF cochin. Spectra were recorded with UV Vis NIR spectro photometer agilent cary 5000 in the region 200nm to 3000nm DRS(Diffuse Intergrating Sphere diameter 150mm angle of incidence 8 0 ) [9].The X-Ray Diffraction(XRD) measurement was performed on X-ray diffractometer(Bruker D8 Advance) operated at 30 Kv and 100 MA and spectrum was recorder by cukα radition with wave length of 1.506 A 0 in the range 200-800nm [10].The surface morphology and size were characterized by Scanning Electron Microscope (SEM)( Joel 6390LA) [11]. Energy Dispersive X-ray (EDX) oxford XMX N operated at 0.5 kV to 30 kV at magnification 300000 and EDX resolution at 136 Ev [12].…”

Haldina cordifolia (Roxb.) Ridsdale Bark Derived Synthesis and Characterization of Silver Nanoparticles: Investigation of its Antituberculosis and Anticancer Activity

“…Recently, use of plants for the synthesis of metal nanoparticles has been encouraged in the field of green chemistry [9]. Primary and secondary metabolites such as proteins, amino acid, vitamins, nucleic acids, alkaloids, terpenoids, flavonoids, saponins and phenols, are all naturally occurring phytoconstituents serving as a greatest source of reducing and capping agents in the nanoparticle synthesis process [10], [11]. Therefore, green synthesis is reliable, clean, biologically appropriate, and environment-friendly technique for the synthesis of metal nanoparticles [12].…”