Biosynthesis of fungal terpenoids

Luo, Pan; Huang, Jia-Hua; Lv, Jian-Ming; Wang, Gao-Qian; Hu, Dan; Gao, Hao

doi:10.1039/d3np00052d

Cited by 20 publications

(9 citation statements)

References 208 publications

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“…STSs have well-known conserved domains that contain aspartate-rich and NSE/DTE motifs, which play important roles in coordinating Mg 2+ to facilitate the ionization of FPP at the active site. , Multiple sequence alignments to determine the presence of D(D/E/N)XX(D/E) and NSE/DTE motifs in these 12 genes helped us determine the function of these STSs. As expected, all contained NSE/DTE signature motifs.…”

Section: Resultsmentioning

confidence: 99%

Functional Characterization of Sesquiterpene Synthases and P450 Enzymes in Flammulina velutipes for Biosynthesis of Spiro [4.5] Decane Terpene

Han,

Zhang,

Xie

et al. 2024

J. Agric. Food Chem.

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Flammulina velutipes, a popular edible mushroom, contains sesquiterpenes with potential health benefits. We characterized 12 sesquiterpene synthases and one P450 enzyme in F. velutipes using Aspergillus oryzae as a heterologous expression system, culminating in the biosynthesis of 16 distinct sesquiterpene compounds. An enzyme encoded by the axeB gene responsible for the synthesis of the spiro [4.5] decane compound axenol was discovered, and the mechanism of spirocycle formation was elucidated through quantum mechanical calculations. Furthermore, we delineated the role of a P450 enzyme colocated with AxeB in producing the novel compound 3-oxo-axenol. Our findings highlight the diverse array of sesquiterpene skeletons and functional groups biosynthesized by these enzymes in F. velutipes and underscore the effectiveness of the A. oryzae system as a heterologous host for expressing genes in the Basidiomycota genome. These insights into the biosynthesis of bioactive compounds in F. velutipes have significant implications for functional food and drug development.

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Section: Resultsmentioning

confidence: 99%

Functional Characterization of Sesquiterpene Synthases and P450 Enzymes in Flammulina velutipes for Biosynthesis of Spiro [4.5] Decane Terpene

Han,

Zhang,

Xie

et al. 2024

J. Agric. Food Chem.

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“…C11 of cyathatriol is subsequently acylated to form 11-O-acetyl cyathatriol, which is then catalyzed by xylosyltransferase to cyathane xyloside, known as erinacine Q. Afterwards, oxidoreductase catalyzes the conversion to erinacine P, which may undergo non-enzymatic conversion to form erinacine B, A, T, and C [ 101 ]. Alternatively, cyathatriol may undergo a glycosyltransferase reaction followed by an oxidoreductase reaction to produce erinacine T ( Scheme 2 ) [ 98 ].…”

Section: Speculated Biosynthesismentioning

confidence: 99%

Unveiling the Chemical Composition and Biofunctionality of Hericium spp. Fungi: A Comprehensive Overview

Kostanda,

Musa,

Pereman

2024

IJMS

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In recent years, research on mushrooms belonging to the Hericium genus has attracted considerable attention due to their unique appearance and well-known medicinal properties. These mushrooms are abundant in bioactive chemicals like polysaccharides, hericenones, erinacines, hericerins, resorcinols, steroids, mono- and diterpenes, and corallocins, alongside essential nutrients. These compounds demonstrate beneficial bioactivities which are related to various physiological systems of the body, including the digestive, immune, and nervous systems. Extensive research has been conducted on the isolation and identification of numerous bioactive chemicals, and both in vitro and in vivo studies have confirmed their antimicrobial, antioxidant, immunomodulatory, antidiabetic, anticholesterolemic, anticancer, and neuroprotective properties. Therefore, this review aims to provide a comprehensive summary of the latest scientific literature on the chemical composition and secondary metabolites profile of Hericium spp. through an introduction to their chemical characteristics, speculated biosynthesis pathways for key chemical families, potential toxicological aspects, and a detailed description of the recent updates regarding the bioactivity of these metabolites.

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“…Furanosteroids are a class of fungal secondary metabolites represented by demethoxyviridin (148), demethoxyviridiol (149), viridin, and wortmannin, 202,203 which are nanomolar-potency inhibitors of phosphatidylinositol 3-kinase (PI3K). 204 (148) or demethoxyviridiol (149), respectively.…”

Section: Furanosteroidsmentioning

confidence: 99%

Recent developments in the enzymatic modifications of steroid scaffolds

Wang,

Abe

2024

Org. Biomol. Chem.

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Biosynthesis of fungal terpenoids

Abstract: This review covers all fungal terpenoid cyclases (TCs), including class I and II TCs as well as emerging UbiA-type TCs, together with their tailoring enzymes, focusing on newly identified TCs and tailoring enzymes from 2015 to August 2023.

Cited by 20 publications

References 208 publications

Functional Characterization of Sesquiterpene Synthases and P450 Enzymes in Flammulina velutipes for Biosynthesis of Spiro [4.5] Decane Terpene

Functional Characterization of Sesquiterpene Synthases and P450 Enzymes in Flammulina velutipes for Biosynthesis of Spiro [4.5] Decane Terpene

Unveiling the Chemical Composition and Biofunctionality of Hericium spp. Fungi: A Comprehensive Overview

Recent developments in the enzymatic modifications of steroid scaffolds

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