Biosynthesis of glutamic acid by immobilized Pseudochrobactrum saccharolyticum in a cowpea waste medium

Abstract

It was tested whether bacteria immobilized on maize cob and corn husk might produce glutamic acid from cowpea waste. Glutamic acid synthesis was tested in bacteria isolates. Organic materials (corn cob and husk) were used as immobilization matrix and then used to produce glutamic acid. The effects of bead size, weight, and reusability were investigated. Glutamic acid production was best with a 2 mm bead size and a 3 kg bead weight. For the surface morphological view of the materials, a scanning electron microscope (SEM) was used. The samples were characterized using Fourier transform infrared (FTIR) analysis. The validity of a high-performance liquid chromatography (HPLC) method for the analysis of amino acids was determined, as well as the quantities of amino acids present. Immobilized Pseudochrobactrum saccharolyticum produced the highest amount of glutamic acid (9.4 g/L). The bacteria cells that had been immobilized were reused over and over again with no noticeable loss of activity. Nineteen amino acids were found, and these amino acids were separated by HPLC analysis. The outcome of the FTIR study revealed a large number of peaks, revealing the glutamic acid's complicated structure. This research found that bacteria immobilized on corn cobs and husks may successfully use lignocellulolytic materials to generate glutamic acid.