Biosynthesis of Glycosaminoglycans

Jourdian, George W.

doi:10.1007/978-1-4613-2925-1_5

Cited by 7 publications

(3 citation statements)

References 91 publications

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“…A critical question which remains to be answered is whether the antigens studied in this report also would fail to increase normally in the absence of presynaptic input. Significant changes in proteoglycan levels have been reported to occur postnatally in rat brain (Margolis et al, 1975;Jourdian, 1979) with the most rapid and striking period occurring between birth and 14 days. Margolis et al (1975) reported that the concentrations of HeS and most other glycosaminoglycans decrease during postnatal development.…”

Section: Discussionmentioning

confidence: 99%

“…Internal elements of dendritic and presynaptic profiles also were stained unevenly, with stain tending to be heavier toward the plasma membrane. This staining may reflect internal processing of the proteoglycan molecule (cf., Jourdian, 1979). There is also biochemical evidence for a large internal pool of this antigen (W. D. Matthew and L. F. Reichardt, manuscript in preparation).…”

Section: Immunocytochemistry Of Antigens In Scgmentioning

confidence: 97%

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Appearance and distribution of neuronal cell surface and synaptic vesicle antigens in the developing rat superior cervical ganglion

Greif¹,

Reichardt²

1982

J. Neurosci.

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Monoclonal antibodies directed against a neuronal cell surface heparan sulfate proteoglycan and against a synaptic vesicle protein were used to study the postnatal development of ganglionic neurons and synapses in the rat superior cervical ganglion. Antigen levels in developing ganglia were quantitated by radioimmune assays. Localization of antigens in adult and developing ganglia was carried out using peroxidase-antiperoxidase immunocytochemistry at the light microscopic level. Ultrastructural staining patterns in adult ganglia also were studied. The time course of antigen increases parallels those in previous reports on the accumulation of neurotransmitter enzymes within the ganglion. Both synaptic and surface antigens increase postnatally, with the most rapid changes occurring during the 2nd week. Antibodies stain adult tissue in patterns consistent with the expected distribution of antigens: antibodies directed against synaptic vesicles stain synaptic terminals and cell cytoplasm and those directed against surface proteoglycan stain the plasma membranes of neuronal cell bodies and processes. Variable staining of the cell cytoplasm also is observed. No apparent changes in antigen distribution are observed with the light microscope during development. Variations in the time course of the development of antigens associated with different portions of the proteoglycan molecule suggest that the intracellular processing of the molecule may vary during development.

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Section: Discussionmentioning

confidence: 99%

Section: Immunocytochemistry Of Antigens In Scgmentioning

confidence: 97%

Appearance and distribution of neuronal cell surface and synaptic vesicle antigens in the developing rat superior cervical ganglion

Greif¹,

Reichardt²

1982

J. Neurosci.

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“…The proteoglycans of the central nervous system are of particular theoretical interest because, unlike those of connective tissues which are in abundance and have considerable structural importance, they dominate the scant extracellular matrix and, therefore, are in an ideal position to influence the interactions and organization of adjacent neurons and glia. Unfortunately, very little is known about these molecules, despite numerous studies of their component glycosaminoglycans in the brain (see reviews: Brunngraber, 1979;Jourdian, 1979;.…”

mentioning

confidence: 99%

Low Buoyant Density Proteoglycans from Saline and Dissociative Extracts of Embryonic Chicken Retinas

Morris

Ting

Birkholz-Lambrecht

1984

Journal of Neurochemistry

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Retinas were labeled in culture with [3H]glucosamine or [3H]leucine and [35S]sulfate and extracted sequentially with physiologically balanced saline and 4 M guanidine HCl. They were dialyzed into associative conditions (0.5 M NaCl) and chromatographed on agarose columns. Under these conditions, some of the proteoglycans were associated in massive complexes that showed low buoyant densities when centrifuged in CsCl density gradients under dissociative conditions (4 M guanidine HCl). Much of the label in these complexes was in molecules other than proteoglycans. Most of the proteoglycans, however, were included on the agarose columns, where they appeared to be constitutionally of low buoyant density. They resisted attempts to separate potential low buoyant density contaminants from the major proteoglycans by direct CsCl density gradient centrifugation or by the fractionation of saline or 8 M urea extracts on diethylaminoethyl-Sephacel. The diethylaminoethyl-Sephacel fractions were either subjected to CsCl density gradient centrifugation or were chromatographed on Sephacryl S-300, in both cases before and after alkaline cleavage, to confirm the presence of typical O-linked glycosaminoglycans. The medium and balanced salt extracts were enriched in chondroitin sulfate and other sulfated macromolecules, possibly highly sulfated oligosaccharides, that resisted digestion by chondroitinase ABC but were electrophoretically less mobile than heparan sulfate. Guanidine HCl or urea extracts of the residues were mixtures of high and low density proteoglycans that were enriched in heparan sulfate.

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Tissue Culture of the Nervous System

Sato¹

1973

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Biosynthesis of Glycosaminoglycans

Cited by 7 publications

References 91 publications

Appearance and distribution of neuronal cell surface and synaptic vesicle antigens in the developing rat superior cervical ganglion

Appearance and distribution of neuronal cell surface and synaptic vesicle antigens in the developing rat superior cervical ganglion

Low Buoyant Density Proteoglycans from Saline and Dissociative Extracts of Embryonic Chicken Retinas

Tissue Culture of the Nervous System

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