Biosynthesis of Gold Nanoparticles and Its Effect against Pseudomonas aeruginosa

Abstract

Antimicrobial resistance has posed a serious health concern worldwide, which is mainly due to the excessive use of antibiotics. In this study, gold nanoparticles synthesized from the plant Tinospora cordifolia were used against multidrug-resistant Pseudomonas aeruginosa. The active components involved in the reduction and stabilization of gold nanoparticles were revealed by gas chromatography–mass spectrophotometry(GC-MS) of the stem extract of Tinospora cordifolia. Gold nanoparticles (TG-AuNPs) were effective against P. aeruginosa at different concentrations (50,100, and 150 µg/mL). TG-AuNPs effectively reduced the pyocyanin level by 63.1% in PAO1 and by 68.7% in clinical isolates at 150 µg/mL; similarly, swarming and swimming motilities decreased by 53.1% and 53.8% for PAO1 and 66.6% and 52.8% in clinical isolates, respectively. Biofilm production was also reduced, and at a maximum concentration of 150 µg/mL of TG-AuNPs a 59.09% reduction inPAO1 and 64.7% reduction in clinical isolates were observed. Lower concentrations of TG-AuNPs (100 and 50 µg/mL) also reduced the pyocyanin, biofilm, swarming, and swimming. Phenotypically, the downregulation of exopolysaccharide secretion from P. aeruginosa due to TG-AuNPs was observed on Congo red agar plates

g‐C3N4@CuO electrostatic self‐assembly toward Ralstonia solanacearum: Insights from cytomembrane and motility disruption

“…Furthermore, copper oxide nanoparticles (CuONPs) are low cost, 24,25 and they exhibit unique physicochemical properties due to their quantum size effect and high specific surface area, which increases the biological and chemical activity of the material 26,27 ; having excellent antimicrobial activity. 28,29 In addition, g-C 3 N 4 nanosheets are non-toxic 23 and CuONPs provide micronutrients 30,31 that promote plant growth advantages and so forth; making it a promising sustainable antimicrobial agent for the future.…”