Biosynthesis of lecithin in brain. Participation of cytidine diphosphate choline and phosphatidic acid

Strickland, K. P.; Subrahmanyam, D.; Pritchard, E.T.; Thompson, W.; Rossiter, R. J.

doi:10.1042/bj0870128

Cited by 37 publications

(10 citation statements)

References 39 publications

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“…Figure 3a and b show that glycerophospholipids (GLs) metabolism was significantly changed in M1 as well as M2. Glycerophospholipids constitute the main components of the cell membrane (Strickland et al 1963). They mainly include phosphatidylcholines (PC), phosphatidylethanolamines (PE), phosphatidylserines (Strickland et al 1963), phosphatidylinositols (PI) and phosphatidylglycerols (PG).…”

Section: Pathway Analysismentioning

confidence: 99%

“…Glycerophospholipids constitute the main components of the cell membrane (Strickland et al 1963). They mainly include phosphatidylcholines (PC), phosphatidylethanolamines (PE), phosphatidylserines (Strickland et al 1963), phosphatidylinositols (PI) and phosphatidylglycerols (PG). The level of GLs in polarised macrophages (M1 and M2) was significantly increased and this results clearly from the increased levels of PC, PS and PE (Supplementary 3 and 4).…”

Section: Pathway Analysismentioning

confidence: 99%

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Metabolic characterisation of THP-1 macrophage polarisation using LC–MS-based metabolite profiling

et al. 2020

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Introduction Macrophages constitute a heterogeneous population of functionally distinct cells involved in several physiological and pathological processes. They display remarkable plasticity by changing their phenotype and function in response to environmental cues representing a spectrum of different functional phenotypes. The so-called M1 and M2 macrophages are often considered as representative of pro-and anti-inflammatory ends of such spectrum. Metabolomics approach is a powerful tool providing important chemical information about the cellular phenotype of living systems, and the changes in their metabolic pathways in response to various perturbations. Objectives This study aimed to characterise M1 and M2 phenotypes in THP-1 macrophages in order to identify characteristic metabolites of each polarisation state. Methods Herein, untargeted liquid chromatography (LC)-mass spectrometry (MS)-based metabolite profiling was applied to characterise the metabolic profile of M1-like and M2-like THP-1 macrophages. Results The results showed that M1 and M2 macrophages have distinct metabolic profiles. Sphingolipid and pyrimidine metabolism was significantly changed in M1 macrophages whereas arginine, proline, alanine, aspartate and glutamate metabolism was significantly altered in M2 macrophages. Conclusion This study represents successful application of LC-MS metabolomics approach to characterise M1 and M2 macrophages providing functional readouts that show unique metabolic signature for each phenotype. These data could contribute to a better understanding of M1 and M2 functional properties and could pave the way for developing new therapeutics targeting different immune diseases.

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Section: Pathway Analysismentioning

confidence: 99%

Section: Pathway Analysismentioning

confidence: 99%

Metabolic characterisation of THP-1 macrophage polarisation using LC–MS-based metabolite profiling

et al. 2020

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“…In the last few years a series of contributions have appeared concerning the synthesis of ethanolamine phosphoglycerides (EPG) in brain tissue in vitro (1)(2)(3)(4), and the role played by the cytidine coenzymes in this metabolic process (1)(2)(3)(4)(5)(6)(7). Only a few studies have examined the in vitro incorporation of labeled precursors into EPG by isolated brain microsomes (3,8), although results obtained both in vitro (3,8) Ipresent address: Ospedale Civile di Como, Como, Italy.…”

Section: Introductionmentioning

confidence: 99%

The incorporation of phosphorylethanolamine into the phospholipids of brain microsomes in vitro

Porcellati

Biasion²,

Arienti

1970

Lipids

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Synthesis of ethanolamine phosphoglycerides (EPG) from labeled14C‐phosphorylethanolamine (PE) and cytidine triphosphate (CTP) has been studied in vitro in particles and in soluble fractions of chicken brain. The microsomal membranes can carry out this conversion, but supplementing the microsomes with an enzymic fraction derived from the particle‐free supernatant results in a noticeable increase of the rate of EPG synthesis. Mitochondria are almost inactive, in this connection. The conversion of PE to lipid is very low, in no case exceeding 0.5–0.6%, even in the presence of diacyl glycerols or 1‐alkenyl 2‐acyl glycerol. No stimulation occurs by supplementing the incubation system with natural lipid acceptors, intermediates of lipid synthesis, energy‐producing cofactors or monoacylsn‐glycero‐3‐phosphorylethanolamine (GPE), monoacylsn‐glycero‐3‐phosphorylcholine (GPC) and other lipid material. From these and other results, the conclusion is made that the PE:CTP cytidylyltransferase (E.C. 2.7.7.14) must display very low activity in vitro, thus limiting the overall rate of synthesis from PE. Diacyl GPE is the only lipid which has been found labeled after incubation with PE of the brain preparations. A small synthesis of alkenyl acyl GPE takes place, only when PE is incubated with suitable concentrations of a plasmalogenic diglyceride.

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“…2). Incorporation of ['40]acetate could arise from the synthesis of phosphatidylcholine by the Kennedy route (Rossiter& Strickland, 1959;Strickland, Subrahmanyam, Pritchard, Thompson & Rossiter, 1963) or by acylation of lysolecithin (Webster, 1965;Webster & Alpern, 1963).…”

Section: Discussionmentioning

confidence: 99%

The metabolism of phospholipids in mouse brain slices

Clayton

Rowe

1966

Biochemical Journal

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1. Slices of mouse brain grey matter were incubated with [(32)P]phosphate and [1-(14)C]acetate. Doubly labelled phospholipids were extracted from subcellular fractions prepared from the slices in a mixture of metabolic inhibitors, under conditions where there was negligible change in radioactive labelling during the preparation. Two tissue fractions were studied in detail; one contained a high proportion of mitochondria and the other was mainly microsomal. 2. In all tissue fractions the highest incorporations of both [(32)P]phosphate and [1-(14)C]acetate occurred into phosphatidylcholine. 3. After incubation for 1hr., the (32)P/(14)C ratios for phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid in the mitochondrial fraction were similar to those in the microsomal fraction. 4. The (32)P/(14)C ratios were similar in phosphatidylcholine and phosphatidylethanolamine and much lower than those in phosphatidic acid and phosphatidylinositol.

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Biosynthesis of lecithin in brain. Participation of cytidine diphosphate choline and phosphatidic acid

Cited by 37 publications

References 39 publications

Metabolic characterisation of THP-1 macrophage polarisation using LC–MS-based metabolite profiling

Metabolic characterisation of THP-1 macrophage polarisation using LC–MS-based metabolite profiling

The incorporation of phosphorylethanolamine into the phospholipids of brain microsomes in vitro

The metabolism of phospholipids in mouse brain slices

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