Biosynthesis of long-chain dicarboxylic acid monomers from renewable resources. Final technical report

Mobley, David P.

doi:10.2172/763082

Cited by 8 publications

(2 citation statements)

References 2 publications

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“…The long-chain dicarboxylic acids are well known platform chemicals for production of various commodities, such as polymers, perfumes, hot-melting adhesives, and high quality lubricants [1][2]. Dodecanedioic acid (DDDA) is one of dicarboxylic acids produced excessively (>40 million pounds per year) [3]. Currently, crude oil-based DDDAs are still used widely as raw material for polymer production, for example nylon which is produced from crude oil through several steps of chemical reaction [4].…”

Section: Introductionmentioning

confidence: 99%

Bioconversion Studies of Methyl Laurate to Dodecanedioic Acid using a Wild-type of Candida tropicalis

2018

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Production of dodecanedioic acid (DDDA), a platform chemical used as raw material for various commodities and polymers, has been studied through a biological process. This process was conducted by using a wild-type of Candida tropicalis which can be obtained easily from natural resources. The aim of this research was to study the characteristics of DDDA production from methyl laurate through batch fermentation process. Growth phase was carried out for 20 h, as the beginning of fermentation, then continued to conversion phase for 5 until 6 days. Utilization of methyl laurate and production of DDDA were analysed using gas chromatography, which proved the ability of C. tropicalis in assimilating methyl laurate to convert it become DDDA. The highest value of cells yield (Yx/s) and DDDA yield (Yp/s) successfully obtained were 0.86 g cells/g methyl laurate and 0.20 g DDDA/g methyl laurate, respectively. This study also showed the possibility of fermentation products accumulation as intermediate, or accumulation of DDDA inside the cells. Thus, this study can be applied as an alternative in addition to the use of mutant microorganism in producing DDDA.

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Section: Introductionmentioning

confidence: 99%

Bioconversion Studies of Methyl Laurate to Dodecanedioic Acid using a Wild-type of Candida tropicalis

2018

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“…Long-chain α,ω-dicarboxylic acids (LDCAs, ≥C12) are widely used as a raw material for preparing various products, such as nylon and other polyamides, polyesters, perfumes, adhesives, and high-quality lubricants. , LDCAs are almost exclusively produced from petrochemical sources by chemical conversion processes that have a number of disadvantages and limitations. These include limitations in the range of carbon chain lengths, use of multistep conversion processes, dependence on nonrenewable petrochemical feedstock, and generation of unwanted and hazardous byproducts. , Biotechnology offers an innovative solution for overcoming these limitations. Fatty acids (FAs) are major constituents of lipids and one of the most abundant renewable resources found in nature.…”

Section: Introductionmentioning

confidence: 99%

Production of Long-Chain α,ω-Dicarboxylic Acids by Engineered Escherichia coli from Renewable Fatty Acids and Plant Oils

Sathesh‐Prabu

Lee

2015

J. Agric. Food Chem.

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Long-chain α,ω-dicarboxylic acids (LDCAs, ≥ C12) are widely used as a raw material for preparing various commodities and polymers. In this study, a CYP450-monooxygenase-mediated ω-oxidation pathway system with high ω-regioselectivity was heterologously expressed in Escherichia coli to produce DCAs from fatty acids. The resulting engineered E. coli produced a maximum of 41 mg/L of C12 DCA and 163 mg/L of C14 DCA from fatty acids (1 g/L), following 20 h of whole cell biotransformation. Addition of a heme precursor and the hydroxyl radical scavenger, thiourea, increased product concentration (159 mg/L of C12 DCA and 410 mg/L of C14 DCA) in a shorter culture duration than that of the corresponding controls. DCAs of various chain lengths were synthesized from coconut oil hydrolysate using the engineered E. coli. This novel synthetic biocatalytic system could be applied to produce high value DCAs in a cost-effective manner from renewable plant oils.

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Selective Microbial Oxidations in Industry: Oxidations of Alkanes, Fatty Acids, Heterocyclic Compounds, Aromatic Compounds and Glycerol Using Native or Recombinant Microorganisms

Weiss¹

2007

Modern Biooxidation

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Biosynthesis of long-chain dicarboxylic acid monomers from renewable resources. Final technical report

Cited by 8 publications

References 2 publications

Bioconversion Studies of Methyl Laurate to Dodecanedioic Acid using a Wild-type of Candida tropicalis

Bioconversion Studies of Methyl Laurate to Dodecanedioic Acid using a Wild-type of Candida tropicalis

Production of Long-Chain α,ω-Dicarboxylic Acids by Engineered Escherichia coli from Renewable Fatty Acids and Plant Oils

Selective Microbial Oxidations in Industry: Oxidations of Alkanes, Fatty Acids, Heterocyclic Compounds, Aromatic Compounds and Glycerol Using Native or Recombinant Microorganisms

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