Biosynthesis of major phospholipids of Microsporum gypseum

Kasinathan, Chinnaswamy; Khuller, O.K.

doi:10.1016/0005-2760(83)90111-x

Cited by 12 publications

(6 citation statements)

References 21 publications

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“…Direct incorporation of [ 4C]choline into PC indicated the cytidine pathway for PC synthesis, which has been shown in other fungi (Kasinathan & Khuller, 1983).…”

Section: Lipid Biosynthesissupporting

confidence: 56%

Lipids of Candida albicans: subcellular distribution and biosynthesis

Mago¹,

Khuller²

1990

Journal of General Microbiology

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Lipids constituted around 5% of the dry weight in Candida albicans 3153, while sterols and phospholipids accounted for 1.2% and 1.1% respectively. Phospholipids were mainly localized in the microsomal fraction; p hosp ha tid y lserine (PS) , p hos p hatid y lcholine ( P o , p hos p hatid y le t hanolamine (PE) and p hosp hatidy linositol (PI) were the major phospholipids. Incorporation studies with [ 14C]acetate and [32P]orthophosphoric acid demonstrated that PS was synthesized at the highest rate followed by PC, PE and PI. There was little difference in either the content or the rate of biosynthesis of PC, PE and PI. Incorporation of labelled serine, ethanolamine and choline revealed serine to be a precursor for PC, PE and PS, ethanolamine for PC and PE, and choline for PC biosynthesis only.

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“…Direct incorporation of [ 4C]choline into PC indicated the cytidine pathway for PC synthesis, which has been shown in other fungi (Kasinathan & Khuller, 1983).…”

Section: Lipid Biosynthesissupporting

confidence: 56%

Lipids of Candida albicans: subcellular distribution and biosynthesis

Mago¹,

Khuller²

1990

Journal of General Microbiology

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“…glycerol kinase and acyltransferase have been well-characterized in dermatophytes (22,23). Biosynthesis of phospholipids takes place primarily by two routes i.e.…”

Section: Biosynthetic Enzymes Of Phospholipidsmentioning

confidence: 99%

“…PC and PE are synthesised by CDP-base pathway while CDP-diacylglycerol pathway is operative for the synthesis of PS, PI and CL. PLs can also be synthesized by an alternative pathway like methylation, base exchange and acylationdeacylation reactions (23)(24)(25). Initially base exchange and PS-decarboxylase were thought to be responsible for PE synthesis in Microsporum gypseum (20) but later on CDP-base pathway was established for its synthesis.…”

Section: Biosynthetic Enzymes Of Phospholipidsmentioning

confidence: 99%

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Dermatophyte lipids—Composition and regulation of phospholipids

Khuller

Sharma

Deo

2000

Indian J Clin Biochem

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This review presents the extensive work carried out on lipid components of dermatophytes, their biosynthesis, turnover and regulation. It emerges from the work done so far that the pathways of lipid biosynthesis/degradation and the lipid composition in dermatophytes are similar to those in yeasts and other fungi. Second messengers (Ca 2 § cAMP) were demonstrated to have a regulatory role in phospholipid metabolism and they mainly act by stimulating Ca2*/CaM or cAMP dependent protein kinase(s). Both these kinases were purified and characterized in Microsporum gypseum. Further work is being carried out to elucidate the molecular mechanism of regulation of phospholipid metabolism by these second messengers.

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“…Cell fractionation and characterization was carried out according to the modified procedure of Chavant et al [5]. Purity of subcellular fractions was checked by their respective marker enzymes as described previously for Microsporum gypseum [ 10].…”

Section: Subcellular Fractionation Of Cellsmentioning

confidence: 99%

Subcellular localization of enzymes of phospholipid metabolism inCandida albicans

Mago

Khuller

1990

Med Mycol

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The intracellular location of various enzymes involved in the metabolism of phospholipids of Candida albicans was studied. Among the biosynthetic enzymes, phosphatidylserine synthetase was found to be localized in the microsomes; choline kinase and ethanolamine kinase were cytosolic; acyltransferase was localized in the paniculate fraction and glycerol kinase and phosphatidic acid phosphatase were distributed in both the microsomal and cytosolic fractions. Phospholipase A and phospholipase C were abundant in the microsomes and phospholipase C was also detected in the cytosol. Lysophospholipase and glycerophosphocholine diesterase were distributed mainly in the mitochondria. Lipase activity was also detected in this fungus. Based on the enzymes detected in this study we have postulated pathways of phospholipid metabolism in C. albicans.Phospholipids are vital structural and functional entities of biomembranes [6] and their metabolism is related to the formation and functions of membrane components [ 1 ]. Biosynthesis of phospholipids occurs mainly via the Kennedy and CDP-diacylglycerol pathways, in addition to alternate routes such as base exchange, methylation, acylation and deacylation reactions. Phospholipid degradation takes place by three main pathways. The first includes phospholipase A, lysophospholipase and glycerophosphocholine diesterase, the second involves phospholipase C, and the third involves phospholipase D and results in the formation of phosphatidic acid. Phosphatidic acid is used either to synthesize triglycerides, or is degraded by lipase for conversion to glycerol-3-phosphate by glycerol kinase. The presence of these pathways has been established in mammalian systems, and to some extent in fungi [8,12,23], but in Candida albicans the information is restricted to extracellular phospholipases [4,19]. This communication describes the identification and subcellular distribution of enzymes of phospholipid metabolism in C. albicans. METHODS ChemicalsRadiolabelled (3H)-glycerol (sp. act. 2-50 mCi mmol-1), (3H)-ethanolamine (sp. act.

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Biosynthesis of major phospholipids of Microsporum gypseum

Cited by 12 publications

References 21 publications

Lipids of Candida albicans: subcellular distribution and biosynthesis

Lipids of Candida albicans: subcellular distribution and biosynthesis

Dermatophyte lipids—Composition and regulation of phospholipids

Subcellular localization of enzymes of phospholipid metabolism inCandida albicans

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