1999
DOI: 10.1074/jbc.274.50.35407
|View full text |Cite
|
Sign up to set email alerts
|

Biosynthesis of Mannosylglycerate in the Thermophilic Bacterium Rhodothermus marinus

Abstract: The biosynthetic reaction scheme for the compatible solute mannosylglycerate in Rhodothermus marinus is proposed based on measurements of the relevant enzymatic activities in cell-free extracts and in vivo 13 C labeling experiments. The synthesis of mannosylglycerate proceeded via two alternative pathways; in one of them, GDP mannose was condensed with D-glycerate to produce mannosylglycerate in a single reaction catalyzed by mannosylglycerate synthase, in the other pathway, a mannosyl-3-phosphoglycerate synth… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

0
31
0

Year Published

2001
2001
2021
2021

Publication Types

Select...
4
2
1

Relationship

2
5

Authors

Journals

citations
Cited by 62 publications
(31 citation statements)
references
References 46 publications
0
31
0
Order By: Relevance
“…The same reasoning is applicable to the MG synthase characterized earlier in R. marinus (15). MPG synthase from P. horikoshii does not share significant amino acid sequence homology with other known sugar transferases nor with MG synthase from R. marinus (15). In view of the apparent peculiarity of the amino acid sequences of these two GDPmannose-dependent enzymes, and the retaining character of their catalytic mechanism, the elucidation of their three-dimensional structures would be most important to reconcile these findings.…”
Section: Pathway For Synthesis Of Mannosylglycerate In P Horikoshii mentioning
confidence: 78%
See 3 more Smart Citations
“…The same reasoning is applicable to the MG synthase characterized earlier in R. marinus (15). MPG synthase from P. horikoshii does not share significant amino acid sequence homology with other known sugar transferases nor with MG synthase from R. marinus (15). In view of the apparent peculiarity of the amino acid sequences of these two GDPmannose-dependent enzymes, and the retaining character of their catalytic mechanism, the elucidation of their three-dimensional structures would be most important to reconcile these findings.…”
Section: Pathway For Synthesis Of Mannosylglycerate In P Horikoshii mentioning
confidence: 78%
“…However, our data show that MPG synthase retains the ␣-configuration of the substrate GDPmannose and therefore cannot be classified as a family 2 glycosyltransferase. The same reasoning is applicable to the MG synthase characterized earlier in R. marinus (15). MPG synthase from P. horikoshii does not share significant amino acid sequence homology with other known sugar transferases nor with MG synthase from R. marinus (15).…”
Section: Pathway For Synthesis Of Mannosylglycerate In P Horikoshii mentioning
confidence: 80%
See 2 more Smart Citations
“…The biosynthetic pathways for MG have been characterized at the genetic and enzyme levels in Pyrococcus horikoshii, Thermus thermophilus, and Rhodothermus marinus (10,11,30). All the microorganisms examined to date use the two-step pathway exclusively with the exception of Rhodothermus marinus, in which the single-step pathway is also functional (5).…”
Section: Discussionmentioning
confidence: 99%