Biosynthesis of metal nanoparticles and their testing on flax seeds

Abstract

This work sets out to investigate a green-synthesized biopreparation produced by introducing iron or copper nanoparticles into a microbial liquid-phase biological product (LBP). The obtained nanoparticles were analyzed by diffuse reflectance IR spectroscopy and introduced into the LPB at the stage of its ripening in the LPB:particle solution volume ratio of 50:1. As a result, two new biopreparations – LPB-Fe and LPB-Cu – were obtained. All LPB samples, as well as iron and copper nanoparticles, underwent laboratory testing on flax seeds of Tverskaya variety. Iron or copper nanoparticles in the composition of biological preparations were found to affect positively the process of seed germination. In the LPB-Fe variant, seed germination ranged between 86–91%, which exceeded that in the control by 3–12%. In the LPB-Cu variant, seed germination ranged between 86–93%, which exceeded that in the control by 3–11%. However, in the latter variant, the maximum average length per seedling was 14.5–14.8 cm. The average length per seedling was significantly affected by watering the seeds with a solution containing only iron nanoparticles, with the maximum value of this parameter reaching 16.1±1.2 cm. According to the results obtained, this research direction has good prospects and requires additional experiments by varying the nanoparticle concentration in LBPs.