Biosynthesis of MgO Nanoparticles Using Lactobacillus Sp. and its Activity Against Human Leukemia Cell Lines HL-60

Mohanasrinivasan, V.; Devi, C. Subathra; Mehra, Avani; Prakash, Suman; Agarwal, Aditi; Selvarajan, E.; Naine, S. Jemimah

doi:10.1007/s12668-017-0480-5

Cited by 23 publications

(20 citation statements)

References 11 publications

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“…The microscopic and electron microscopic views were used to find the substrate mycelium and true branching of filamentous structures. The filamentous structure confirmed that the isolated microorganisms were actinobacteria, which is in accordance with many previous studies [10,19,23,29].…”

Section: Isolation and Screening Of Actinobacteria From Magnesite Minsupporting

confidence: 92%

“…Then, the broth was placed on a shaking incubator at 28 C for 7 days. After the incubation period, biomasses were observed and centrifuged at 5000 rpm at 4 C for 20 min to separate the supernatant [14,19,20,23,30]. The supernatant was filtered through Whatmann No.…”

Section: Microbial Synthesis Of Mgo Npsmentioning

confidence: 99%

“…Similarly, SSA10 culture showed an excellent reduction in all the chemicals used for this test. After that, all the precipitates were filtered and washed with double-distilled water several times and then dried [18,19,27]. The dried precipitates were sintered at 500 C for 1 h and termed as SA8 and SA10.…”

Section: Microbial Reduction Of Magnesium Oxidementioning

confidence: 99%

“…Hence, all the prepared samples were selected for further studies. [18,19,28]. Similarly, the XRD patterns of C100 and S100 clearly suggest the presence of a hexagonal structure of Mg (OH) NPs determined with diffraction peaks at 2h values 18.54(001), 32.80(110), 37.95(101), 50.93(102), 58.61(110), 62.02(111), 68.03(103), 72.04(012) (JCPDS file numbers 84-2163 and 002-1207) [11,28,29].…”

Section: Characterization Studiesmentioning

confidence: 99%

“…Besides the applicative aspects of nanoparticles, another domain that is of great concern is the risk assessment of these nanoparticles to avoid unwanted hazards. Many of the researchers have shown the toxicity of metal oxide NPs in humans [18,19]; therefore, we have used the biological method to synthesize the NPs, which provides stability to nanoparticles and is cost-effective, eco-friendly, as well as non-toxic [14,18,19]. Both unicellular and multicellular organisms such as bacteria, fungi, and yeast produce inorganic materials either intra-cellularly or extra-cellularly [14,[20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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Reliability of antioxidant potential and in vivo compatibility with extremophilic actinobacterial-mediated magnesium oxide nanoparticle synthesis

Kandiah

Jeevanantham

Balagurunathan

2019

Artificial Cells, Nanomedicine, and Biotechnology

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Owing to the hazards of chemical and physical syntheses of magnesium oxide nanoparticles (MgO NPs), an eco-friendly, high-yield, and promising biological method is highly desirable for biomedical applications. Hence, in this study, an extremophilic actinobacterial population (SA8 and SA10) from Salem magnesite mining soil was used as precursors for MgO NP synthesis. The prepared nanoparticles were subjected to X-ray diffraction study and showed face-centred cubic structure with an average particle size of 18-24 nm. Among all, high yield was obtained in SA10 actinobacteria-mediated synthesis of MgO NPs (480 mg/100 mL). In addition, the prepared MgO NPs (10 mg/well) showed 15-17 mm zone of inhibition against bacterial pathogens, especially bigger zones around SA10. The 64.5% antioxidant activity and nonsignificant toxicity of actinobacteria-synthesized MgO NPs in MG-63 cell lines at 100 mg/mL and nonsignificant in vivo toxicity in zebrafish at 0.1 mg/mL were remarkable. In addition, this is the first study to focus on MgO NP synthesis using extremophilic actinobacteria collected from Salem magnesite mining soil for high yield (115 mg/100 mL), reliable with potential antioxidant, and in vitro and in vivo compatibility. These results provided useful information for advanced research and mass production of NP for biomedical applications. GRAPHICAL ABSTRACT ARTICLE HISTORY

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Section: Isolation and Screening Of Actinobacteria From Magnesite Minsupporting

confidence: 92%