Biosynthesis of Nonspecific Lipid Transfer Proteins in Germinating Castor Bean Seeds

Tsuboi, Shigeru; Watanabe, Shinichiro; Ozeki, Yoshihiro; Yamada, Mitsuhiro

doi:10.1104/pp.90.3.841

Cited by 11 publications

(3 citation statements)

References 21 publications

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“…Our results are consistent with previous observations showing that the amount of LTP, determined by ELISA and immunoblotting methods, varies during maturation and germination of maize (Grosbois et al, 1989) bean (Tsuboi et al, 1989). These authors observed the same kinetics for LTP accumulation and enhancement of phospholipid transfer activity.…”

Section: Discussion Ltp Gene Expression 1s Temporally and Spatially Rsupporting

confidence: 83%

Spatial and Temporal Expression of a Maize Lipid Transfer Protein Gene

Sossountzov¹,

Ruiz-Avila²,

Vignols³

et al. 1991

The Plant Cell

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We studied the temporal and spatial pattern of lipid transfer protein (LTP) gene expression, as well as the localization of this protein, in maize. Using an LTP gene, we observed an accumulation of LTP mRNA in embryos and endosperms during seed maturation. LTP gene expression was also investigated in young seedlings. After germination, the leve1 of LTP mRNA in the coleoptile increased, with a maximum at 7 days, whereas LTP mRNA levels were low in the scutellum and negligible in roots. The high levels of LTP mRNA found in coleoptiles and embryos were confirmed by in situ hybridization. Moreover, LTP gene expression appeared to be localized in the externa1 cellular layers and around the leaf veins. Using immunogold methods, we also observed that LTP was distributed heterogeneously in the different cells of coleoptiles and leaves. The highest concentrations of LTP were found in the outer epidermis of the coleoptiles as well as in the leaf veins. Together, our observations indicate that LTP gene expression is not only organ specific and time specific but also cell specific.

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Section: Discussion Ltp Gene Expression 1s Temporally and Spatially Rsupporting

confidence: 83%

Spatial and Temporal Expression of a Maize Lipid Transfer Protein Gene

Sossountzov¹,

Ruiz-Avila²,

Vignols³

et al. 1991

The Plant Cell

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“…It is known that different isoforms of LTPs can coexist in a given plant – . Furthermore, LTPs are known to exhibit post-translational modifications such as phosphorylation as in the case of LTPs from wheat and barley .…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Two Lipid Transfer Proteins in Pomegranate (Punica granatum)

Zoccatelli

Pellegrina²,

Consolini³

et al. 2007

J. Agric. Food Chem.

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Lipid transfer proteins (LTPs) are a family of low molecular mass (7-9 kDa) polypeptides, the members of which share 35-95% sequence homology. These proteins are widely distributed throughout the plant kingdom and are receiving attention for their biochemical characteristics and biological activity. LTPs are indeed studied in different research fields varying from allergy to food technology, and numerous molecules belonging to this class are progressively being identified and investigated. Proteins from pomegranate juice were fractioned by cation exchange chromatography and analyzed by SDS-PAGE. Two proteins were identified as putative LTPs on the basis of their molecular weights and their electrophoretic behaviors under reducing and nonreducing conditions. Finally, proteins were purified and characterized by mass spectrometry. This analysis confirmed that the two polypeptides are LTPs on the basis of an amino acid sequence common to LTPs from other plant sources and cysteine content. The two proteins, named LTP1a and LTP1b, showed similar molecular masses but different immunological profiles when immunodetected with rabbit antibodies specific for Pru p 3 and human IgE from a patient suffering from pomegranate allergy. The demonstration of the existence of two immunologically unrelated LTPs in pomegranate confirms the variability and the complexity of the plant LTP family. This should be taken into account when the role of these proteins as elicitors of allergies to fruits is investigated and could help to explain the contradictory literature data on pomegranate allergy.

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“…The availability of polyclonal antibodies prepared against LTPs purified from various plants or fusion proteins (105,112) has provided tools to confirm, in some cases, the nature of a putative LTP (105), to determine the LTP contents either by ELISA or Western blot (40,116), or to localize several LTPs immunohistochemically (104,112). These antibodies also helped in the isolation of cDNAs encoding plant LTPs (109,115).…”

Section: Purificationmentioning

confidence: 99%

Lipid-Transfer Proteins in Plants

Kader

1996

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

870

695

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Lipid-transfer proteins (LTP) are basic, 9-kDa proteins present in high amounts (as much as 4% of the total soluble proteinss) in higher plants. LTPs can enhance the in vitro transfer of phospholipids between membranes and can bind acyl chains. On the basis of these properties, LTPs were thought to participate in membrane biogenesis and regulation of the intracellular fatty acid pools. However, the isolation of several cDNAs and genes revealed the presence of a signal peptide indicating that LTPs could enter the secretory pathway. They were found to be secreted and located in the cell wall. Thus, novel roles were suggested for plant LTPs: participation in cutin formation, embryogenesis, defense reactions against phytopathogens, symbiosis, and the adaptation of plants to various environmental conditions. The validity of these suggestions needs to be determined, in the hope that they will elucidate the role of this puzzling family of plant proteins.

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Biosynthesis of Nonspecific Lipid Transfer Proteins in Germinating Castor Bean Seeds

Cited by 11 publications

References 21 publications

Spatial and Temporal Expression of a Maize Lipid Transfer Protein Gene

Spatial and Temporal Expression of a Maize Lipid Transfer Protein Gene

Isolation and Identification of Two Lipid Transfer Proteins in Pomegranate (Punica granatum)

Lipid-Transfer Proteins in Plants

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