Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01

Kim, Do Young; Park, Doo-Sang; kwon, Soon Bum; Chung, Moon Gyu; Bae, Kyung Sook; Park, Ho-Yong; Rhee, Young Ha

doi:10.1007/s12275-009-0109-7

Cited by 16 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3). It has been proposed that HV production was significantly influenced by suitable C:N ratio [28], [31]. More importantly, 54% HV content could be produced at a low concentration of DO; however, only 30.6% HV content was synthesized at a high concentration of DO (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…The PHBV content was much higher than that achieved by Burkholderia sp. IS-01 in a 7-L fermenter, which contained gluconate (20 g L −1 ) and LA (5 to 12.5 g L −1 ) as the co-substrates [31]. Additionally, although high concentration of LA is known to be toxic to microorganisms and to lead to a decrease in growth, there was a significant increase in the DCW of R. eutropha as the concentration of LA was increased from 2 to 8 g L −1 in this study (Fig.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biosynthesis and Thermal Properties of PHBV Produced from Levulinic Acid by Ralstonia eutropha

et al. 2013

View full text Add to dashboard Cite

Levulinic acid (LA) can be cost-effectively produced from a vast array of renewable carbohydrate-containing biomaterials. LA could facilitate the commercialization of the polymer poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and PHBV-based products as carbon substrates. Therefore, this paper focused on the production of PHBV by Ralstonia eutropha with LA for hydroxyvalerate (HV) production, which plays an important role in enhancing the thermal properties of PHBV. Accordingly, the HV content of PHBV varied from 0–40.9% at different concentrations of LA. Stimulation of cell growth and PHBV accumulation were observed when 2–6 g L−1 LA was supplied to the culture. The optimal nitrogen sources were determined to be 0.5 g L−1 ammonium chloride and 2 g L−1 casein peptone. It was determined that the optimal pH for cell growth and PHBV accumulation was 7.0. When the cultivation was performed in large scale (2 L fermenter) with a low DO concentration of 30% and a pH of 7.0, a high maximum dry cell weight of 15.53 g L−1 with a PHBV concentration of 12.61 g L−1 (53.9% HV), up to 81.2% of the dry cell weight, was obtained. The melting point of PHBV found to be decreased as the fraction of HV present in the polymer increased, which resulted in an improvement in the ductility and flexibility of the polymer. The results of this study will improve the understanding of the PHBV accumulation and production by R. eutropha and will be valuable for the industrial production of biosynthesized polymers.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Biosynthesis and Thermal Properties of PHBV Produced from Levulinic Acid by Ralstonia eutropha

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Although these cosubstrates are toxic to bacterial cells even at relatively low concentrations, the 3HV fraction in the copolyester increases generally as the concentration of the cosubstrate in the culture medium increases (Choi et al, 2003;Kim et al, 2009). In the present study, the effect of cosubstrates on PHA production and its monomeric composition was investigated.…”

Section: Effect Of Valerate As the 3hv Precursormentioning

confidence: 84%

Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain

Woo¹,

Kim²,

Ni³

et al. 2012

The Korean Journal of Microbiology

View full text Add to dashboard Cite

A bacterial strain capable of synthesizing polyhydroxyalkanoates (PHAs) with an unusual pattern of monomer units was isolated from activated sludge using the enrichment culture technique. The organism, identified as Pseudomonas aeruginosa P-5, produced polyesters consisting of 3-hydroxyvalerate and medium-chain-length (MCL) 3-hydroxyalkanoate monomer units when C-odd alkanoic acids such as nonanoic acid and heptanoic acid were fed as the sole carbon source. Solvent fractionation experiments using chloroform and hexane revealed that the 3-hydroxyalkanoate monomer units in these polyesters were copolymerized. The molar concentration of 3-hydroxyvalerate in the polyesters produced were significantly elevated up to 26 mol% by adding 1.0 g/L valeric acid as the cosubstrate. These copolyesters were sticky with low degrees of crystallinity. The PHA synthase genes were cloned, and the deduced amino acid sequences were determined. P. aeruginosa P-5 possessed genes encoding MCL-PHA synthases (PhaC1 and PhaC2) but lacked the short-chain-length PHA synthase gene, suggesting that the MCL-PHA synthases from P. aeruginosa P-5 are uniquely active for polymerizing (R)-3-hydroxyvaleryl-CoA as well as MCL (R)-3-hydroxyacyl-CoAs.

show abstract

“…Bacteria capable of using levulinic acid as the 3HV precursor are rare and are mainly C. necator, with the exception of Burkholderis sp. IS-01 and Hydrogenophaga pseudoflava DSM 1034 [10,11,[65][66][67][68]. C. necator KHB-8862 and H. pseudoflava DSM 1034 are two promising strains reported with a high 3HV yield of 0.50 and 1.00 g/g, respectively.…”

Section: Bioconversion Of Alkyl Alcohols and Organic Acids Into P(3hb...mentioning

confidence: 99%

A Critical Review on the Economically Feasible and Sustainable Poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) Production from Alkyl Alcohols

2022

View full text Add to dashboard Cite

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) is the most studied short-chain-length polyhydroxyalkanoates (PHA) with high application importance in various fields. The domination of high-cost propionate and valerate over other 3-hydroxyvalerate (3HV) precursors owing to their wide preference among PHA-producing bacteria has hindered the development of diverse production processes. As alkyl alcohols are mainly produced from inexpensive starting materials through oxo synthesis, they contribute a cost-effective advantage over propionate and valerate. Moreover, alkyl alcohols can be biosynthesized from natural substrates and organic wastes. Despite their great potential, their toxicity to most PHA-producing bacteria has been the major drawback for their wide implementation as 3HV precursors for decades. Although the standard PHA-producing bacteria Cupriavidus necator showed promising alcohol tolerance, the 3HV yield was discouraging. Continuous discovery of alkyl alcohols-utilizing PHA-producing bacteria has enabled broader choices in 3HV precursor selection for diverse P(3HB-co-3HV) production processes with higher economic feasibility. Besides continuous effort in searching for promising wild-type strains, genetic engineering to construct promising recombinant strains based on the understanding of the mechanisms involved in alkyl alcohols toxicity and tolerance is an alternative approach. However, more studies are required for techno-economic assessment to analyze the economic performance of alkyl alcohol-based production compared to that of organic acids.

show abstract

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01

Cited by 16 publications

References 14 publications

Biosynthesis and Thermal Properties of PHBV Produced from Levulinic Acid by Ralstonia eutropha

Biosynthesis and Thermal Properties of PHBV Produced from Levulinic Acid by Ralstonia eutropha

Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain

A Critical Review on the Economically Feasible and Sustainable Poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) Production from Alkyl Alcohols

Contact Info

Product

Resources

About