Biosynthesis of precious metabolites in callus cultures of Eclipta alba

Khurshid, Razia; Khan, Taimoor; Zaeem, Afifa; Garros, Laurine; Hano, Christophe; Abbasi, Bilal Haider

doi:10.1007/s11240-018-1463-0

Cited by 13 publications

(21 citation statements)

References 34 publications

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“…Constant temperature (25 ± 2˚C) and light (8 dark/16 light) conditions were maintained in growth room for proper germination of seeds. Stem explant was taken from 28 days old plantlets and placed in hormonally optimized MS media, supplemented with 3.0 mg/L BAP (6-benzylaminopurine) and 1.0 mg/L NAA (αnaphthalene acetic acid) as previously studied by Khurshid et al [26]. Explant inoculated culture flasks were placed under aseptic conditions in growth room (Photoperiod: 8 dark/16 light and Temperature: 25 ± 2˚C) for 4 weeks.…”

Section: In Vitro Seed Germination and Callus Inductionmentioning

confidence: 99%

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Lights triggered differential accumulation of antioxidant and antidiabetic secondary metabolites in callus culture of Eclipta alba L.

et al. 2020

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Eclipta alba L., also known as false daisy, is well known and commercially attractive plant with excellent hepatotoxic and antidiabetic activities. Light is considered a key modulator in plant morphogenesis and survival by regulating important physiological cascades. Current study was carried out to investigate growth and developmental aspects of E. alba under differential effect of multispectral lights. In vitro derived callus culture of E. alba was exposed to multispectral monochromatic lights under controlled aseptic conditions. Maximum dry weight was recorded in culture grown under red light (11.2 g/L) whereas negative effect was observed under exposure of yellow light on callus growth (4.87 g/L). Furthermore, red light significantly enhanced phenolics and flavonoids content (TPC: 57.8 mg/g, TFC: 11.1 mg/g) in callus cultures compared to rest of lights. HPLC analysis further confirmed highest accumulation of four major compounds i.e. coumarin (1.26 mg/g), eclalbatin (5.00 mg/g), wedelolactone (32.54 mg/g) and demethylwedelolactone (23.67 mg/g) and two minor compounds (β-amyrin: 0.38 mg/g, luteolin: 0.39 mg/g) in red light treated culture whereas stigmasterol was found optimum (0.22 mg/g) under blue light. In vitro based biological activities including antioxidant, antidiabetic and lipase inhibitory assays showed optimum values in cultures exposed to red light, suggesting crucial role of these phytochemicals in the enhancement of the therapeutic potential of E. alba. These results clearly revealed that the use of multispectral lights in in vitro cultures could be an effective strategy for enhanced production of phytochemicals.

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Section: In Vitro Seed Germination and Callus Inductionmentioning

confidence: 99%

“…HPLC quantification of metabolites. Quantification of the major E. alba callus phytochemicals was performed according to Khurshid et al [26]. Briefly, RP-HPLC was used for separation and quantification, equipped with chromatographic Compounds of E. alba were detected at 204 nm.…”

Section: Phytochemical Analysismentioning

confidence: 99%

Lights triggered differential accumulation of antioxidant and antidiabetic secondary metabolites in callus culture of Eclipta alba L.

et al. 2020

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“…Plant specialized metabolites of pharmacological significance may be difficult to extract in high, sustainable and continuous quantities from field-grown plant material, but in vitro cultures have been identified as a possible biological system for the production of these metabolites [ 12 ]. In vitro culture techniques provide consistent production schemes for metabolites that are free from environmental limitations and ensure increased biomass and a high concentration of compounds compared to natural production systems [ 13 ]. In addition, in the case of basil varieties or cultivars which can be differentiated on the basis of color, aromatic composition and growth habitat, sometimes harvested from nature, thus complicating its botanical identity, the in vitro system presents a major advantage for the certification of plant authentication [ 4 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Callus Culture of Thai Basil Is an Effective Biological System for the Production of Antioxidants

et al. 2020

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Thai basil is a renowned medicinal plant and a rich source of bioactive antioxidant compounds with several health benefits, with actions to prevent of cancer, diabetes and cardiovascular disease. Plant cell and tissue culture technologies can be routinely established as an important, sustainable and low-cost biomass source to produce high-value phytochemicals. The current study aimed at developing an effective protocol to produce Thai basil leaf-derived callus cultures with sustainable and high production of biomass and antioxidants as an alternative of leaves production. MS basal medium with various concentrations of plant growth regulators (PGRs) compatible with nutraceutical applications (i.e., gibberellic acid (GA3) and 6-benzylaminopurine (BAP) either alone or in combination with naphthalene acetic acid (NAA)) were evaluated. Among all tested PGRs, the combination BAP:NAA (5 mg/L:1 mg/L) yields the maximum biomass accumulation (fresh weight (FW): 190 g/L and dry weight (DW): 13.05 g/L) as well as enhanced phenolic (346.08 mg/L) production. HPLC quantification analysis indicated high productions of chicoric acid (35.77 mg/g DW) and rosmarinic acid (7.35 mg/g DW) under optimized callus culture conditions. Antioxidant potential was assessed using both in vitro cell free and in vivo cellular antioxidant assays. Maximum in vitro antioxidant activity DPPH (93.2% of radical scavenging activity) and ABTS (1322 µM Trolox equivalent antioxidant capacity) was also observed for the extracts from callus cultures grown in optimal conditions. In vivo cellular antioxidant activity assay confirmed the effective protection against oxidative stress of the corresponding extract by the maximum inhibition of ROS and RNS production. Compared to commercial leaves, callus extracts showed higher production of chicoric acid and rosmarinic acid associated with higher antioxidant capacity. In addition, this biological system also has a large capacity for continuous biomass production, thus demonstrating its high potential for possible nutraceutical applications.

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“…Plant-specialized metabolites that are responsible for health attributes have high demand in pharmaceutical industries [ 3 , 15 , 16 ]. But most of the time, the production capacity of the natural source does not meet industrial criteria for direct exploitation.…”

Section: Introductionmentioning

confidence: 99%

“…Competitive benefits of these cultures include rapid production of phytochemicals, irrespective of environmental and seasonal constraints, without geographical limits, disease free, easy harvesting and specific material production make plant tissue culturing highly desirable [ 18 ]. Research has emphasized developing strategies for improved growth of plants with sufficient yield of medicinal compounds to reduce the threats of depleting plant resources, raised by the overexploitation of plants in their natural habitat [ 15 ]. Various plant growth regulators (PGRs) have been explored and found with altering growth, morphology, and metabolite accumulation in callus cultures [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Scarlet Flax Linum grandiflorum (L.) In Vitro Cultures as a New Source of Antioxidant and Anti-Inflammatory Lignans

et al. 2021

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In vitro cultures of scarlet flax (Linum grandiflorum L.), an important ornamental flax, have been established as a new possible valuable resource of lignans and neolignans for antioxidant and anti-inflammatory applications. The callogenic potential at different concentrations of α-naphthalene acetic acid (NAA) and thidiazuron (TDZ), alone or in combinations, was evaluated using both L. grandiflorum hypocotyl and cotyledon explants. A higher callus induction frequency was observed on NAA than TDZ, especially for hypocotyl explants, with a maximum frequency (i.e., 95.2%) on 1.0 mg/L of NAA. The presence of NAA (1.0 mg/L) in conjunction with TDZ tended to increase the frequency of callogenesis relative to TDZ alone, but never reached the values observed with NAA alone, thereby indicating the lack of synergy between these two plant growth regulators (PGRs). Similarly, in terms of biomass, NAA was more effective than TDZ, with a maximum accumulation of biomass registered for medium supplemented with 1.0 mg/L of NAA using hypocotyls as initial explants (DW: 13.1 g). However, for biomass, a synergy between the two PGRs was observed, particularly for cotyledon-derived explants and for the lowest concentrations of TDZ. The influence of these two PGRs on callogenesis and biomass is discussed. The HPLC analysis confirmed the presence of lignans (secoisolariciresinol (SECO) and lariciresinol (LARI) and neolignan (dehydrodiconiferyl alcohol [DCA]) naturally accumulated in their glycoside forms. Furthermore, the antioxidant activities performed for both hypocotyl- and cotyledon-derived cultures were also found maximal (DPPH: 89.5%, FRAP 866: µM TEAC, ABTS: 456 µM TEAC) in hypocotyl-derived callus cultures as compared with callus obtained from cotyledon explants. Moreover, the anti-inflammatory activities revealed high inhibition (COX-1: 47.4% and COX-2: 51.1%) for extract of hypocotyl-derived callus cultures at 2.5 mg/L TDZ. The anti-inflammatory action against COX-1 and COX-2 was supported by the IC50 values. This report provides a viable approach for enhanced biomass accumulation and efficient production of (neo)lignans in L. grandiflorum callus cultures.

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Biosynthesis of precious metabolites in callus cultures of Eclipta alba

Cited by 13 publications

References 34 publications

Lights triggered differential accumulation of antioxidant and antidiabetic secondary metabolites in callus culture of Eclipta alba L.

Lights triggered differential accumulation of antioxidant and antidiabetic secondary metabolites in callus culture of Eclipta alba L.

Callus Culture of Thai Basil Is an Effective Biological System for the Production of Antioxidants

Scarlet Flax Linum grandiflorum (L.) In Vitro Cultures as a New Source of Antioxidant and Anti-Inflammatory Lignans

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