Biosynthesis of Rauscher leukemia viral proteins

Naso, Robert; Arcement, L.J.; Arlinghaus, Ralph B.

doi:10.1016/0092-8674(75)90130-0

Cited by 148 publications

(76 citation statements)

References 9 publications

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“…Immunoprecipitation of p2lras products was accomplished with a monoclonal antibody designated Y13-259, developed in the laboratory of E. Scolnick (22) and donated by Dr. Scolnick, and was performed by the method of Furth et al (22) with modifications. Briefly, 2.5 mg of lyophilized tissue was dissolved in 1 ml of detergent-containing buffer as described by Naso et al (23). Lysates were cleared by centrifuging at 10,000 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Expression of p21ras in fresh primary and metastatic human colorectal tumors.

Gallick

Kurzrock

Kloetzer

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

161

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Activation of the cellular oncogene ras has been implicated in many types of human malignancies. In this study, the relative levels of p21 protein product of ras (p2lr") in primary and metastatic colon tumors were compared to those in adjacent normal tissues. Nine of the 17 primary tumors had substantially elevated levels of p2lr, with respect to adjacent normal tissues. Eight of these tumors were from Dukes' B and C stages. Four of the five tumors classified as "D" stage (in which distant metastases are present) did not show elevated levels of p21r,. In metastases from primary colon tumors, nine of nine were considerably reduced in p2l' expression regardless of the site of metastasis. These data suggest that elevation of p2lr" may be a common event in early stages of colon tumors, and tumor progression may lead to a more autonomous population of cells in which other growth factors supplant the role of this protein.

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Section: Methodsmentioning

confidence: 99%

Expression of p21ras in fresh primary and metastatic human colorectal tumors.

Gallick

Kurzrock

Kloetzer

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

161

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“…After gentle mixing, the nuclear fraction was separated by low-speed centrifugation (1500 r.p.m., 10 min), and the supernatant, subsequently referred to as the perinuclear cytoskeletal fraction, was collected. The cytoplasmic fraction was further separated into the NP40-soluble and the insoluble pellet fractions by centrifugation at 10000g for 15 min (Naso et al, 1975).…”

Section: Cells and Rirusmentioning

confidence: 99%

On the Intracellular Transport and the Nuclear Association of Human Cytomegalovirus Structural Proteins

Yamauchi¹,

Nishiyama²,

Fujioka

et al. 1985

Journal of General Virology

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SUMMARYIn cells productively infected with human cytomegalovirus (HCMV) AD169, large amounts of two viral proteins, the 150K major capsid and the 68K major matrix proteins, are continuously produced during the late phase of infection. In the present study, the mechanism for the intracellular transport of the 150K and 68K proteins was investigated. Infected cells were labelled for 30 rain at 72 h post-infection with [3SS]methionine, chased for various periods of time at 37 °C, and fractionated into cytoplasmic and nuclear fractions. Immediately after 30 min of labelling, the 68K protein was already associated with the nuclear fraction. In contrast, the major proportion of the 150K protein remained in the cytoplasm for more than 1 h; the migration of the 150K protein was much slower than that of the 68K protein. Both the 150K and the 68K proteins were associated with the perinuclear cytoskeletal fraction in the process of migration. After migration into the nucleus, these proteins were resistant to extraction with DNase and high salt, indicating that they were associated with the nuclear skeleton (nuclear matrix). Effects of various inhibitors on the migration of the 150K protein showed that cycloheximide inhibited the transport of the 150K protein, but other inhibitors such as arabinosyl cytosine, cytochalasin D, colchicine or sodium azide did not. The results suggest that the cytoskeletal structure may play a role in the intracellular transport of HCMV structural proteins from the cytoplasm into the nucleus.

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“…These conditions are routinely employed for binding of immune complexes. Nearly confluent monolayer cultures (75 cm 2) of uninfected NIH Swiss mouse cells (V16), R-MuLV-infected V16 cells (Naso et al, 1975) and MuLV-infected 3T3 cells were disrupted in 3 ml cell lysis buffer (20 mM-tris-HC1 pH 7.5, 1.4 mM-MgC12, 3.6 mM CaC12, 120 mMNaC1, 5 mM-KC1, 0.5% NP40, 0.5% sodium deoxycholate; Naso et al, 1975). After lowspeed centrifugation to remove most cell debris, the cell lysate was clarified by spinning at 67000 g for 90 min.…”

mentioning

confidence: 99%

Binding of Retrovirus-associated Protein Kinase and Proteins to Staphylococcus aureus

Kloetzer

Arlinghaus

1982

Journal of General Virology

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SUMMARYFormalin-fixed Staphylococcus aureus strain Cowan, bearing protein A, routinely used for the absorption of antigen-antibody complexes, was found to bind protein kinase activity from disrupted Moloney murine leukaemia virus (Mo-MuLV). The Wood strain of S. aureus lacking protein A also bound the kinase with similar efficiency. About 50% of the bound kinase activity, as detected by phosphorylation of casein using [y-32p]ATP, could be eluted from the bacterial preparation with buffer containing 0.5 M-KC1. Similar results were obtained with Moloney routine sarcoma virus (Mo-MuSV) strain 349 and tsll0 MuSV(MuLV). The bacterial preparation was also found to bind casein kinase activity from cellular extracts of uninfected, Rauscher murine leukaemia virus (R-MuLV)-infected and Mo-MuLVinfected cells. Analysis of [3H]leucine-labelled proteins from purified virus showed selective binding to S. aureus of only two major labelled virus proteins. One virus component bound to S. aureus had the relative mobility of p15; the other polypeptide co-migrated with virus pl0. Upon exposure to increased salt concentration, most of the pl0 but very little of the p15 proteins were released. The S. aureusbinding proteins from tsll0 Mo-MuSV and MuSV-349 revealed similar binding and elution patterns of p 10 and p 15 molecules. The p 10 and protein kinase activity eluted from Mo-MuLV-absorbed bacteria were separated by gel filtration into a high molecular weight species, containing pl0 and kinase activity, and a low molecular weight p 10 monomer lacking enzymic activity.

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Biosynthesis of Rauscher leukemia viral proteins

Cited by 148 publications

References 9 publications

Expression of p21ras in fresh primary and metastatic human colorectal tumors.

Expression of p21ras in fresh primary and metastatic human colorectal tumors.

On the Intracellular Transport and the Nuclear Association of Human Cytomegalovirus Structural Proteins

Binding of Retrovirus-associated Protein Kinase and Proteins to Staphylococcus aureus

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