Biosynthesis of Rosmarinic Acid in Cell Cultures ofColeus blumei

Abstract

NADH 190iM NADPH 95M position 3 of the phenolic ring occurs only at the ester stage of RA formation. HPPR is competitively inhibited by RA and pyruvate. Caffeic acid, p-coumaric acid, and cinnamic acid do not affect the enzyme activity, p-coumaroyl-CoA inhibits HPPR. The back reaction, i.e. the oxidation of pHPL to the corresponding pyruvate, takes place only to a small extent.

Induction of Vanillic Acid Synthesis in Cell Suspension Cultures ofVanilla planifolia; Proposal of a Biosynthetic Pathway

“…Proposal of a Biosynthetic Pathway P. Brodelius' and C. Funk' 1 Institute of Biotechnology, ETH-HOnggerberg, Switzerland Cell cultures of Vanilla planfolia Mdr. (Orchidaceae) have been used to study the biosynthesis of benzoate derivatives.…”

Hydroxyphenylpyruvate Reductase: An Enzyme of Rosmarinic Acid Biosynthesis in Cell Cultures ofColeus blumei

“…This is the first report of the preparative isolation of this compound from higher plant tissue. 1 Bentley, R., Meganathan, R. (1983) In cell suspension cultures of Coleus blumei Benth. (Lamiaceae) the synthesis of rosmarinic acid (HA) can be induced by raising the initial sucrose content of the culture medium from 2% to 4% (1).…”

“…1 Bentley, R., Meganathan, R. (1983) In cell suspension cultures of Coleus blumei Benth. (Lamiaceae) the synthesis of rosmarinic acid (HA) can be induced by raising the initial sucrose content of the culture medium from 2% to 4% (1). Under these conditions the cells acanthraquinone cumulate up to 20% of their dry weight as RA.…”