Biosynthesis of selenium nanoparticles by lactic acid bacteria and areas of their possible applications

Stabnikova, Olena; Khonkiv, Myroslav; Kovshar, Iryna; Stabnikov, Viktor

doi:10.1007/s11274-023-03673-6

Cited by 13 publications

(6 citation statements)

References 118 publications

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“…Additionally, after incubation of E. faecium ABMC-05 in the presence of Na 2 SeO, the culture medium and biomass turned red. The color became more intense as the fermentation time elapsed, suggesting that this bacterium, like other LAB, reduces Na 2 SeO 3 to Se 0 , the less toxic form of inorganic selenium [6,[43][44][45].…”

Section: Determination Of Selenium Accumulation In Bacteria By Icpmentioning

confidence: 99%

“…LAB were reported to accumulate Se and then transform into organic selenite compounds such a selenocysteine through detoxification [7,36,46]. Thus, the detoxification process is directly related to the degree of toxicity that Se exerts on the bacteria, and it is known that the detoxification speed increases at higher toxicity levels [6,[43][44][45][46][47]. Unlike other species of the genus Enterococcus reported by other authors [13,35], the behavior of E. faecium ABMC-05 is very similar to that reported by Lampis et al [42] for Stenotrophomonas maltophilia SeITE02.…”

Section: Determination Of Selenium Accumulation In Bacteria By Icpmentioning

confidence: 99%

“…LAB were reported to accumulate Se and then transform into organic selenite compounds such as selenocysteine through detoxification [7,36,46]. Thus, the detoxification process is directly related to the degree of toxicity that Se exerts on the bacteria, and it is known that the detoxification speed increases at higher toxicity levels [6,[43][44][45][46][47].…”

Section: Determination Of Selenium Accumulation In Bacteria By Icpmentioning

confidence: 99%

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Selenocysteine Formation by Enterococcus faecium ABMC-05 Follows a Mechanism That Is Not Dependent on Genes selA and selD but on Gene cysK

et al. 2023

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Lactic acid bacteria (LAB) resist sodium selenite of concentrations greater than 100 mg/L in fermentation media. Selenium affects the growth rate, but once the microorganism absorbs selenium, this element is converted through a complex mechanism into selenocysteine and then into a selenoprotein structure. This study verified the presence of selenocysteine in Enterococcus faecium ABMC-05. The microorganism was cultivated in a medium enriched with a minimum inhibitory concentration of sodium selenite (184 mg/L). The concentration of selenium absorbed and the bioconversion into selenocysteine were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) and reverse-phase high-performance chromatography (RP-HPLC), respectively. The presence of the selD, selA, and cysK genes was determined by amplifying the 16S rDNA through polymerase chain reaction (PCR). The microorganism accumulated inorganic selenium, and part was transformed into selenocysteine. The growth curves were atypical for a lactic acid bacterium with a stationary phase greater than 70 h. Determining the genetic expression showed only the presence of the cysK gene and the absence of the selD and the selA genes. The results demonstrate that this microorganism produces selenocysteine through a mechanism independent of the SelA and SelD pathways in contrast to other LAB.

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Section: Determination Of Selenium Accumulation In Bacteria By Icpmentioning

confidence: 99%

Section: Determination Of Selenium Accumulation In Bacteria By Icpmentioning

confidence: 99%

Section: Determination Of Selenium Accumulation In Bacteria By Icpmentioning

confidence: 99%

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Selenocysteine Formation by Enterococcus faecium ABMC-05 Follows a Mechanism That Is Not Dependent on Genes selA and selD but on Gene cysK

et al. 2023

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“…However, when the energy potential is depleted in the stationary phase, selenite is able to persist in the cytoplasm for a sufficient time and turn into SeNPs. Selenium nanoparticle synthesis at the beginning of the stationary phase also involves many lactobacilli [70]. Additionally, the cellular reductase production and consumption involved in selenite reduction are mediated by the bacteria's growth phase.…”

Section: By Bacteriamentioning

confidence: 99%

Selenium Nanoparticles: Green Synthesis and Biomedical Application

Mikhailova

2023

Molecules

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Selenium nanoparticles (SeNPs) are extremely popular objects in nanotechnology. “Green” synthesis has special advantages due to the growing necessity for environmentally friendly, non-toxic, and low-cost methods. This review considers the biosynthesis mechanism of bacteria, fungi, algae, and plants, including the role of various biological substances in the processes of reducing selenium compounds to SeNPs and their further packaging. Modern information and approaches to the possible biomedical use of selenium nanoparticles are presented: antimicrobial, antiviral, anticancer, antioxidant, anti-inflammatory, and other properties, as well as the mechanisms of these processes, that have important potential therapeutic value.

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“…The biological method depends on the green chemistry that can be performed by living organisms or their natural secondary metabolites such as proteins including plants [ 24 , 25 ], fungi [ 26 ], yeast [ 27 ], algae [ 28 ], non-living viruses [ 29 ] and bacteria [ 23 , 30 , 31 ]. Among the significant categories of microorganisms involved in biosynthesis of NPs, the probiotic lactic acid bacteria (LAB) are recommended as a safe, rapid, easy to culture, available in dairy products and cheap bio-nano-factory for NPs production [ 32 , 33 ]. They can be facultative anaerobes, even though they are sometimes classified as aerotolerant anaerobes.…”

Section: Introductionmentioning

confidence: 99%

Anticandidal applications of selenium nanoparticles biosynthesized with Limosilactobacillus fermentum (OR553490)

Mohamed,

El‑Zahed

2024

Discover Nano

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Candida albicans is one of the most dangerous pathogenic fungi in the world, according to the classification of the World Health Organization, due to the continued development of its resistance to currently available anticandidal agents. To overcome this problem, the current work provided a simple, one-step, cost-effective, and safe technique for the biosynthesis of new functionalized anticandidal selenium nanoparticles (Se NPs) against C. albicans ATCC10231 using the cell-free supernatant of Limosilactobacillus fermentum (OR553490) strain. The bacterial strain was isolated from yogurt samples available in supermarkets, in Damietta, Egypt. The mixing ratio of 1:9 v/v% between cell-free bacterial metabolites and sodium selenite (5 mM) for 72 h at 37 °C were the optimum conditions for Se NPs biosynthesis. Ultraviolet–visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), Zeta analyses, and elemental analysis system (EDS) were used to evaluate the optimized Se NPs. The Se NPs absorption peak appeared at 254 nm. Physicochemical analysis of Se NPs revealed the crystalline-shaped and well-dispersed formation of NPs with an average particle size of 17–30 nm. Se NPs have − 11.8 mV, as seen by the zeta potential graph. FT-IR spectrum displayed bands of symmetric and asymmetric amines at 3279.36 cm−1 and 2928.38 cm−1, aromatic and aliphatic (C–N) at 1393.32 cm−1 and 1237.11.37 cm−1 confirming the presence of proteins as stabilizing and capping agents. Se NPs acted as a superior inhibitor of C. albicans with an inhibition zone of 26 ± 0.03 mm and MIC value of 15 µg/mL compared to one of the traditional anticandidal agent, miconazole, which revealed 18 ± 0.14 mm and 75 µg/mL. The cytotoxicity test shows that Se NPs have a low toxic effect on the normal keratinocyte (IC50 ≈ 41.5 μg/mL). The results indicate that this green synthesis of Se NPs may have a promising potential to provide a new strategy for drug therapy.

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Biosynthesis of selenium nanoparticles by lactic acid bacteria and areas of their possible applications

Cited by 13 publications

References 118 publications

Selenocysteine Formation by Enterococcus faecium ABMC-05 Follows a Mechanism That Is Not Dependent on Genes selA and selD but on Gene cysK

Selenocysteine Formation by Enterococcus faecium ABMC-05 Follows a Mechanism That Is Not Dependent on Genes selA and selD but on Gene cysK

Selenium Nanoparticles: Green Synthesis and Biomedical Application

Anticandidal applications of selenium nanoparticles biosynthesized with Limosilactobacillus fermentum (OR553490)

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