Biosynthesis of Selenium Nanoparticles using yeast Nematospora coryli and examination of their anti‐candida and anti‐oxidant activities

Abstract

Selenium (Se) is a rare and essential element for the human body and other living organisms because of its role in the structure of several proteins and having anti-oxidant properties to reduce oxidative stress at cells. Some microorganisms can absorb Se oxyanions and convert them into zero-valent Se (Se0) in the nanoscale dimensions, which can be used for producing Se nanoparticles (SeNPs). In the present study, SeNPs were intracellularly biosynthesised by yeast Nematospora coryli, which is an inexpensive method and does not involve using materials hazardous for human and environment. The produced NPs were refined by a two-phase system and then characterised and identified by ultraviolet-visible, X-ray diffraction, X-ray fluorescence, transmission electron microscope, and Fourier transform infrared spectroscopy analyses. The structural analysis of biosynthesised SeNPs showed spherical-shaped NPs with size ranging from 50 to 250 nm. Also, extracted NPs were applied to explore their anti-candida and anti-oxidant activities. The results of this investigation confirm the biological properties of Se.

Biosynthesis and antioxidation of nano-selenium using lemon juice as a reducing agent

“…Biological synthesis includes plant and microbial synthesis, among which microbial synthesis is more applied [24]. Rasouli [25] synthesized spherical selenium nanoparticles with particle sizes between 50 and 250 nm by yeast nematode yeast in cells. The prepared particles have biological characteristics of resistance and antioxidant activity of Candida (a pathogenic bacteria).…”

Green synthesis of selenium nanoparticles and evaluate their effect on the expression of ERG3, ERG11 and FKS1 antifungal resistance genes in Candida albicans and Candida glabrata

“…It was observed that the NPs peaks were somewhat milder. It concluded that the proteins might have formed a coating over the nano‐selenium, supporting their stabilization (Rasouli 2019).…”

Green Synthesis of Selenium and Tellurium Nanoparticles: Current Trends, Biological Properties and Biomedical Applications

“…On the other hand, the extracellular processes have the advantage of easy biogenic NP recovery over their intracellular counterparts [211]. According to Rassouli, the general procedure for the extraction and purification of yeastproduced SeNPs consists of (i) applying some enzymatic, chemical, or mechanic method to destroy the cell wall; (ii) collecting the biomass by centrifugation at 8000 rpm for 10 min; (iii) crushing the cells using liquid nitrogen and ultrasounds; (iv) incubating the broken cells with added buffer at 60 • C for 10 min; (v) mixing the pellet containing the cell fragments and NPs with octanol and distilled water to give rise to two phases of which (vi) the SeNP-containing top phase is recovered and further washed with ethanol and chloroform [48].…”

“…Their features, such as the size, morphology, chemical composition, surface functionality, and crystallinity, play an important role in determining their potential applications in numerous fields, such as biomedicine, nanobiotechnology, agriculture, pharmacology, optoelectronics, etc. [48][49][50]. Over the past few years, selenium and tellurium have become chalcogenides of great interest owing to their unique photoconductive and thermoconductive properties [51].…”