Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

Chauhan, Ritika; Reddy, A. Varada; Abraham, Jayanthi

doi:10.1007/s13204-014-0292-7

Cited by 100 publications

(38 citation statements)

References 18 publications

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“…The possible reaction mechanism of ZnO formation is the reduction of zinc ion by the phytochemicals such as alkaloids, flavonoids, terpenoids, and tannins present in the plant extract and acts as bioreductant as well as a stabilizing and capping agent to finally produce ZnO nanoparticles. The obtained SPR peak at 375 nm was highly correlated with the previous studies on green synthesis of ZnO NPs [12,16]. The optical band-gap energy was calculated using Tauc's equation (Eq.…”

Section: Uv-vis Analysissupporting

confidence: 80%

Fabrication of hollow particles composed of silica containing gadolinium compound and magnetic resonance imaging using them

et al. 2013

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A facile, eco-friendly synthesis of zinc oxide nanoparticles (ZnO NPs) employing Bauhinia tomentosa leaf extract as bioreducing agent was reported. The green-synthesized ZnO NPs were characterized by UV-Vis, TEM, EDX, XRD, and FTIR analyses. The formation of ZnO NPs was confirmed by the appearance of characteristic SPR peak at 370 nm due to the collective oscillation of electrons in the conduction band in UV-Vis spectra. The hexagonal morphology exhibiting nanosized ZnO was observed from the TEM and XRD analyses. The chemical bonds present in the as-synthesized ZnO NPs were identified by FTIR analysis. ZnO NPs showed a significant antibacterial activity against Gram-negative bacteria P. aeruginosa and E. coli than Gram-positive bacteria. Results of this study demonstrated that B. tomentosa leaf extract containing phytochemicals such as alkaloids, terpenoids, flavonoids, tannins, carbohydrates, and sterols possess bioreducing property for ZnO synthesis and the obtained ZnO NPs could be employed effectively as a better bactericidal agent for biological applications. Graphical abstract

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Section: Uv-vis Analysissupporting

confidence: 80%

Fabrication of hollow particles composed of silica containing gadolinium compound and magnetic resonance imaging using them

et al. 2013

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“…Secondly, the synthesized ZnONPs were capped by these metabolites thereby stabilizing them. In literature, several reports concluded that extracellular synthesis of ZnONPs is a nitrate reductase‐mediated synthesis, which is responsible for the reduction of Zn ions into ZnONPs (Kundu et al ; Chauhan et al ; Kalpana et al ; Gao et al ). The reduction of Zn ions starts through electron transfer from NADH by NADH‐dependent reductase that acts as an electron carrier (Hulkoti and Taranath ).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the synthesized ZnONPs exhibited potent antimicrobial activity compared to the standard antimicrobials (amoxicillin/clavulanic acid and nystatin). Previous reports have demonstrated the antimicrobial activity of ZnONPs against several bacterial and fungal species; P. aeruginosa (Jayaseelan et al ; Rajabairavi et al ), Enterococcus sp., S. aureus and P. mirabilis (Chauhan et al ), E.coli and B. subtilis (Balraj et al ), S. aureus and E. coli (Khatami et al ), B. subtilis , E. coli and P. fluorescens (Jiang et al ), H. pylori (Saravanan et al ), A. flavus (Jayaseelan et al ), A. alternata , A. niger , B. cinerea , F. oxysporum and P. expansum (Jamdagni et al ), B. subtilis , S. aureus , E. faecalis , E. coli , P. aeruginosa , K. pneumoniae , C. albicans and C. tropicalis (Abd Elkodous et al ; Gao et al ). Our results further showed that the estimated MIC value of the synthesized ZnONPs against the tested microbes was in the range of 50–100 µg ml −1 .…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of zinc oxide nanoparticles with antimicrobial, anticancer, antioxidant and photocatalytic activities by the endophytic Alternaria tenuissima

2020

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Aims Zinc oxide nanoparticles (ZnONPs) were successfully synthesized using the culture filtrate of the endophytic fungus Alternaria tenuissima as a rapid, eco‐friendly and cost‐effective method. Methods and Results The rapid synthesis of ZnONPs was completed after 20 min as confirmed by UV–Vis spectroscopy. The synthesized ZnONPs showed a single‐phase crystalline structure. Dynamic light scattering analysis showed that the synthesized ZnONPs were monodispersed and the recorded polydispersity index value was 0·311. Zeta potential value of –23·92 mV indicated the high stability of ZnONPs. Transmission electron microscope revealed the spherical shape and the mean particle size was 15.45 nm. Functional groups present in the prepared samples of ZnONPs were confirmed by Fourier transform infrared spectroscopy. Additionally, the biological activities of in vitro antimicrobial, anticancer, antioxidant as well as the photocatalytic activities were evaluated. ZnONPs showed broad spectrum of antimicrobial potential against all the tested plant and human pathogens. Based on the MTT assay, ZnONPs inhibited the proliferation of normal human melanocytes, human breast and liver cancer cell lines with IC50 concentrations of 55·76, 18·02 and 16·87 µg ml−1. ZnONPs exhibited promising antioxidant potential with 50% inhibitory concentration of 102·13 µg ml−1. Moreover, ZnONPs showed efficient degradation of methylene blue dye. Conclusions The synthesized ZnONPs showed promising activities that can be better explored in the near future for many medical, agricultural and industrial applications. Significance and Impact of the Study This study suggests a new and alternate approach with the excellent biotechnological potentiality for the production of ZnONPs which could open up the way for the industrial manufacture of nanoparticles using microbial platforms.

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“…The extracellular biosynthesis of silver and zinc oxide nanoparticles was investigated using Pichia fermentans JA2 by Chauhan et al (2015). The biosynthesized silver nanoparticles were found to inhibit most Gram-negative clinical pathogens used in the study, while zinc oxide nanoparticles only inhibited P. aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Punjabi

Mehta

Chavan³

et al. 2018

Front. Microbiol.

100

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Biosynthesis of metallic nanoparticles has acquired particular attention due to its economic feasibility, low toxicity, and simplicity of the process. In this study, extracellular synthesis of silver and zinc nanoparticle was carried out by Pseudomonas hibiscicola isolated from the effluent of an electroplating industry in Mumbai. Characterization studies revealed synthesis of 40 and 60 nm nanoparticles of silver (AgNP) and zinc (ZnNP), respectively, with distinct morphology as observed in TEM and its crystalline nature confirmed by XRD. DLS, zeta potential, NTA, and FTIR studies further characterized nanoparticles giving data about its size, stability, and functional groups. Considering the toxicity of nanoparticles the evaluation of antimicrobial activity was studied in the range of non-toxic concentration for normal cell lines. Silver nanoparticles were found to be the most effective antimicrobial against all tested strains and drug-resistant clinical isolates of MRSA, VRE, ESBL, MDR, Pseudomonas aeruginosa with MIC in the range of 1.25–5 mg/ml. Zinc nanoparticles were found to be specifically active against Gram-positive bacteria like Staphylococcus aureus including its drug-resistant variant MRSA. Both AgNP and ZnNP were found to be effective against Mycobacterium tuberculosis and its MDR strain with MIC of 1.25 mg/ml. The synergistic action of nanoparticles assessed in combination with a common antibiotic gentamicin (590 μg/mg) used for the treatment of various bacterial infections by Checker board assay. Silver nanoparticles profoundly exhibited synergistic antimicrobial activity against drug-resistant strains of MRSA, ESBL, VRE, and MDR P. aeruginosa while ZnNP were found to give synergism with gentamicin only against MRSA. The MRSA, ESBL, and P. aeruginosa strains exhibited MIC of 2.5 mg/ml except VRE which was 10 mg/ml for both AgNPs and ZnNPs. These results prove the great antimicrobial potential of AgNP and ZnNP against drug-resistant strains of community and hospital-acquired infections and opens a new arena of antimicrobials for treatment, supplementary prophylaxis, and prevention therapy.

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Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

Cited by 100 publications

References 18 publications

Fabrication of hollow particles composed of silica containing gadolinium compound and magnetic resonance imaging using them

Fabrication of hollow particles composed of silica containing gadolinium compound and magnetic resonance imaging using them

Biosynthesis of zinc oxide nanoparticles with antimicrobial, anticancer, antioxidant and photocatalytic activities by the endophytic Alternaria tenuissima

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

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