Biosynthesis of Silver Nanoparticles from Penicillium sps and their potential antioxidant activity

Abstract

Nanotechnology is a field of science that involves the production of particles with at least one dimension less than 100 nm. These nanoparticles have found various applications in the field of biomedicine, diagnostic, antimicrobial and anticancer agents etc. The present investigation focuses on the development of economical and eco-friendly methods for the biosynthesis of Silver Nanoparticles using cell free extract of Penicillium sps. The production of nanoparticles was monitored by scanning through UV Visible Spectrophotometer in the range of 300-70 nm. Size and shape of nanoparticles were determined by Scanning Electron Microscope (SEM) while the elemental composition was studied by Energy- dispersive X-ray spectroscopy (EDX). The presence of various functional groups that might have contributed for stability of silver nanoparticles was determined by Fourier transform infrared spectroscopy (FTIR). Antioxidant activities of silver nanoparticles were studied by 2,2- diphenylpicrylhydrazyl (DPPH) scavenging, Hydrogen peroxide (H2 O2 ) radical scavenging and Reducing Power Assay (RPA). The nanoparticles showed dose dependent increase in antioxidant activities with IC50 value of 314.47 + 4.96 µg/ml and 317.34 + 4.40 µg/ml respectively for DPPH scavenging and H2 O2 radical scavenging activity