Biosynthesis of silver nanoparticles from Cavendish banana peel extract and its antibacterial and free radical scavenging assay: a novel biological approach

Abstract

Biosynthesis of metallic silver nanoparticles has now become an alternative to physical and chemical approaches. In the present study, silver nanoparticles (AgNPs) were synthesized from Cavendish banana peel extract (CBPE) and characterized by UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, Atomic force microscopy (AFM), Field emission scanning electronic microscope (FESEM), Dynamic light scattering (DLS) and zeta potential (ZP). The AgNPs formation was confirmed by UV-visible spectroscopy through color conversion due to surface plasma resonance band at 430 nm. The effect of pH on nanoparticle synthesis was determined by adjusting the various pH of the reaction mixtures. The crystalline nature of nanoparticles was confirmed from the XRD pattern, and the grain size was found to be around 34 nm. To identify the compounds responsible for the bioreduction of Ag ? ion and the stabilization of AgNPs produced, the functional group present in Cavendish banana peel extract was investigated using FTIR. AFM has proved to be very helpful in determining morphological features and the diameter of AgNPs in the range of 23-30 nm was confirmed by FESEM. DLS studies revealed that the average size of AgNPs was found to be around 297 nm. Zeta potential value for AgNPs obtained was -11 mV indicating the moderate stability of synthesized nanoparticles. The antibacterial activity of the nanoparticles was studied against Gram-positive and Gram-negative bacteria. Biosynthesized AgNPs showed a strong DPPH radical and ABTS scavengers compared to the aqueous peel extract of Cavendish banana.

One-step preparation of banana powder/silver nanoparticles composite films

“…ions to Ag-NPs, while the starch acted as a stabilizing agent for the Ag-NPs (Patil et al 2012;Vigneshwaran et al 2006). Similar observations in green synthesis of Ag-NPs using plant extracts have been reported by many researchers (Donda et al 2013;Kokila et al 2015;Prakash et al 2013;Shankar et al 2014a;Shinde et al 2014). …”

Electrospun PCL membranes incorporated with biosynthesized silver nanoparticles as antibacterial wound dressings

“…Our previous report demonstrated that silver nanoparticles can successfully be synthesized in a facile and cost-effective way using leaf extracts of Piper nigrum (Augustine et al 2014e). Biosynthesized silver nanoparticles were reported for their excellent antibacterial activity against wide range of microorganisms (Gnanadesigan et al 2012;Kathiraven et al 2014;Netala et al 2014;Sinha et al 2014;Kumar et al 2015;Kokila et al 2015;Roy et al 2014). Antifungal activity of biosynthesized silver nanoparticles is also well studied (Kumar et al 2013;Medda et al 2014).…”

Biogenic synthesis of silver nanoparticles from waste banana plant stems and their antibacterial activity against Escherichia coli and Staphylococcus Epidermis

“…The difference between chemically produced and biologically synthesis Ag nanoparticles has also been reported by Kokila et al, for banana peel extract. 36 The present study revealed Escherichia coli (gramnegative) displayed a larger inhibition zone compared with Staphylococcus Epidermis (gram-positive). The most likely cause for this difference is due to the variation in cell wall composition of the two-gram types.…”