Biosynthesis of silver nanoparticles from curd whey for its anticancer activity

Abstract

Nowadays extracellular synthesis of metallic nanoparticles by microorganisms are considered as active biosynthesis protocol. In this study we have successfully synthesized and characterized silver nanoparticles from curd whey, which is rich with several extracellular proteins and enzymes. Whey is easiest and simplest technique used for the synthesis of size-controlled silver nanoparticles (AgNPs). The mechanism of production of silver nanoparticles by curd whey is hypothesized that, mainly the enzymes produced by lactic acid bacteria as reductase or nitrate reductase have a great role in the bio-reduction of silver ions to AgNPs.The production of AgNPs from whey was confirmed by the colour variation from milky white to brown. Using UV-visible spectrophotometer, the biosynthesized AgNPs indicated an absorption sharp peak at 320 nm.Scanning electron microscopic (SEM) analysis showed that the AgNPs varied in shape and were partly aggregated. The particle size ranged from 30-100nm. Energy dispersive X-ray spectroscopy (EDX) confirms the purity of AgNPs. The FTIR analysis indicated the presence of functional groups such as hydroxyl, carboxyl, amine, ester, aldehydes and these groups may be responsible for the reduction, capping, and stabilization of the AgNPs.Acute myeloid leukemia (AML) is characterized by clonal Biosynthesis… Ashajyothi C et al.