Biosynthesis of silver nanoparticles mediated by entomopathogenic bacteria: Bacillus cereus VCRC 641 against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus

Abstract

Mosquito-borne diseases are illnesses caused by the transmission of viruses, parasites, and protozoa to humans by mosquito vectors. Mosquitoes spread various diseases, including, dengue fever, malaria, chikungunya, West Nile fever, Zika and yellow fever. Bacterial DNA and cellular proteins were shown to help produce AgNPs, with bacterial DNA acting as a stabilising agent and cellular proteins acting as both a reducing and stabilising agent. As a result, the bacterial lysate, which contains a lot of DNA and proteins, could help in the creation of AgNPs that are larvicidal against mosquito larvae. Therefore, this work was aimed to study the larvicidal effect of synthesized AgNPs by Bacillus cereus VCRC 641 cell lysate. The present study demonstrates the larvicidal efficacy of silver nanoparticles (AgNPs) produced by Bacillus cereus VCRC 641 against the diseases-causing mosquitoes Aedes aegypti, Culex quinquefasciatus, and Anopheles stephensi. Silver nano particles were synthesized using Bacillus cereus to enhance its mosquitocidal efficacy. The level of toxicity in the AgNPs synthesized B. cereus was increased 117 fold in comparison with the cell lysate.