Biosynthesis of Silver Nanoparticles Using Parachlorella Kessleri and Cyclotella Spp and Evaluation of its Antibacterial Activity

Ouardy, Khadija El; Lbouhmadi, Rkia; Attaoui, Hind; Mouzaki, Mustapha; Mouine, Hanane; Lemkhente, Zohra; Mir, Youssef

doi:10.2139/ssrn.4222633

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“…Microalgae-mediated nanomaterials present enormous potential for large-scale production, with promising applications in different sectors [34]. Examples of microalgae species already reported to be useful for AgNP production include Chlamydomonas reinhardtii [43], Desmodesmus abundans [44], Parachlorella kessleri [45], various chlorophytes and charophytes [35], and different isolates of Botryococcus, Chlorella, Coelastrum, and Scenedesmus, among others [38]. Since the physicochemical characteristics and biological properties of nanoparticles depend on the organism and production methods used, the search for new microalgae able to produce AgNPs with different applications-for instance, antimicrobial activity-is highly recommended.…”

Section: Introductionmentioning

confidence: 99%

Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches

Pernas-Pleite,

Conejo-Martínez,

Fernández Freire

et al. 2023

IJMS

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The era of increasing bacterial antibiotic resistance requires new approaches to fight infections. With this purpose, silver-based nanomaterials are a reality in some fields and promise new developments. We report the green synthesis of silver nanoparticles (AgNPs) using culture broths from a microalga. Broths from two media, with different compositions and pHs and sampled at two growth phases, produced eight AgNP types. Nanoparticles harvested after several synthesis periods showed differences in antibacterial activity and stability. Moreover, an evaluation of the broths for several consecutive syntheses did not find relevant kinetics or activity differences until the third round. Physicochemical characteristics of the AgNPs (core and hydrodynamic sizes, Z-potential, crystallinity, and corona composition) were determined, observing differences depending on the broths used. AgNPs showed good antibacterial activity at concentrations producing no or low cytotoxicity on cultured eukaryotic cells. All the AgNPs had high levels of synergy against Escherichia coli and Staphylococcus aureus with the classic antibiotics streptomycin and kanamycin, but with ampicillin only against S. aureus and tetracycline against E. coli. Differences in the synergy levels were also dependent on the types of AgNPs. We also found that, for some AgNPs, the killing of bacteria started before the massive accumulation of ROS.

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