Biosynthesis of silver nanoparticles with antimicrobial and anticancer properties using two novel yeasts

Abstract

AgNPs are nanomaterials with many potential biomedical applications. In this study, the two novel yeast strains HX-YS and LPP-12Y capable of producing biological silver nanoparticles were isolated. Sequencing of ribosomal DNA-ITS fragments, as well as partial D1/D2 regions of 26S rDNA indicated that the strains are related to species from the genus Metschnikowia. The BioAgNPs produced by HX-YS and LPP-12Y at pH 5.0–6.0 and 26 °C ranged in size from 50 to 500 nm. The antibacterial activities of yeast BioAgNPs against five pathogenic bacteria were determined. The highest antibacterial effect was observed on P. aeruginosa, with additional obvious effects on E. coli ATCC8099 and S. aureus ATCC10231. Additionally, the BioAgNPs showed antiproliferative effects on lung cancer cell lines H1975 and A579, with low toxicity in Beas 2B normal lung cells. Therefore, the AgNPs biosynthesized by HX-YS and LPP-12Y may have potential applications in the treatment of bacterial infections and cancer.

Silver Nanoparticles: Bactericidal and Mechanistic Approach against Drug Resistant Pathogens

“…Silver ions are continuously released from silver nanoparticles, which may consider a mechanism for killing microbes. Silver ions can easily adhere to the cell wall and cytoplasmic membrane as they are more closely related to sulfur proteins and also due to electrostatic attraction [ 54 , 67 , 68 , 69 , 70 , 71 , 72 ]. At the same time, the bacterial envelope is disrupted because when silver ions attach to the cell wall or cytoplasmic membrane, it enhances the permeability of the cell and ultimately leads to cell disruption.…”

Strong Antimicrobial Activity of Silver Nanoparticles Obtained by the Green Synthesis in Viridibacillus sp. Extracts

“…Developing resistance mechanisms in pathogenic microorganisms against current and developing drugs has become a prime concern in the medical field. Understanding the developing resistance mechanisms in these pathogens is important, and designing novel and strong antimicrobial agents that can overcome or circumvent the resistance is equally important ( Liu et al, 2021 ). Indeed, with exposure to novel antimicrobial agents, there are always opportunities for microbes to become unresponsive or resistant.…”

Novel Antibacterial, Cytotoxic and Catalytic Activities of Silver Nanoparticles Synthesized from Acidophilic Actinobacterial SL19 with Evidence for Protein as Coating Biomolecule

“…The biosynthesis of silver nanoparticles (AgNPs) using microorganisms such as bacteria, fungi, cyanobacteria, actinomycetes, and algae offers low production costs and high efficiency [ 1 ]. Biomolecules derived from microorganisms have been successfully utilized for the reduction of metal ions to nanoparticles (NPs), as in the case of Ag + [ 2 , 3 ].…”

“…In the green chemistry of AgNPs, biomolecules are also involved in the coating of NPs [ 3 ], thereby preventing their agglomeration which significantly decreases the bioactivity and cell association that is crucial in medical applications [ 4 , 5 ]. It is believed that biogenic AgNPs are biocompatible due to natural capping, which is an advantage compared to physically and chemically produced AgNPs [ 1 ]. However, the coating of biomolecules such as proteins may influence the antimicrobial and cellular cytotoxicity exerted by AgNPs [ 6 , 7 ].…”