Biosynthesis of Silver–Silver Chloride Nanoparticles Using Fruit Extract of Levisticum Officinale: Characterization and Anticancer Activity Against MDA-MB-468 Cell Lines

Sattari, Roshana; Khayati, Gholam Reza; Hoshyar, Reyhane

doi:10.1007/s10876-020-01818-3

Cited by 11 publications

(9 citation statements)

References 32 publications

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“…4(a)). In fact, the nanoparticles are highly stable in the solution, if the density of negative or positive charges at the surface of Ag/AgCl nanocomposites is lower than −20 mV or higher than 20 mV [31,32]. The elemental compositions were investigated by EDS mapping (Fig.…”

Section: Resultsmentioning

confidence: 99%

Facile Green Synthesis of Ag/Agcl Nanocomposite Using Durian Shell Extract and Its Activity Against Methicillin-Resistant Staphylococcus Aureus;

Nguyen¹,

Nguyen²,

Trinh³

et al. 2022

Nano BioMed ENG

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The green synthesis of Ag/AgCl nanocomposite using the durian shell extract was carried out under the sunlight illumination at room temperature, showing the face-centered cubic crystalline structures of both Ag and AgCl with nanosizes in the range of 15-25 nm. The high negative potential value of the Ag/AgCl solution (-21.1 mV) established the high dispersion of Ag/AgCl, long-term stability, and good colloidal nature. The antibacterial activity of Ag/AgCl nanocomposite against methicillinresistant Staphylococcus aureus (MRSA) bacteria was highly appreciated with average inhibition zone diameter of 12.0 mm, minimum inhibitory concentration of 8.27 mg/L, and minimum bactericidal concentration of 66.1 mg/L. The present work designed a cost-effective, convenient, eco-friendly protocol to synthesize Ag/AgCl nanocomposite and its efficient antibacterial activity against MRSA was evidenced as well.

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Section: Resultsmentioning

confidence: 99%

Facile Green Synthesis of Ag/Agcl Nanocomposite Using Durian Shell Extract and Its Activity Against Methicillin-Resistant Staphylococcus Aureus;

Nguyen¹,

Nguyen²,

Trinh³

et al. 2022

Nano BioMed ENG

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“…However, a limited number of investigations have showen the cytotoxicity effect of the biologically fabricated silver chloride nanoparticles toward cell lines in vitro. Sattari et al (2020) using Levisticum O cinale extract, reported that Ag and Ag chloride nanoparticles were toxic to MDA-MB-468 breast cancer cells (Sattari et al 2020). Moreover, charelli et al (2018 in their study revealed that silver chloride nanoparticles from B. megaterium have a little effect on toxicity of human adipose tissue stem cells (ASCs) in spheroid 3D culture.…”

Section: Evaluation Of Cell Viability Assaymentioning

confidence: 99%

An Eco-friendly Fabrication of Silver Chloride Nanoparticles (AgClNPs) using Onopordum acanthium L. extract Induces Apoptosis in Breast Cancer MDA-MB-232 Cells

2022

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In the current experimental work, silver chloride nanoparticles (AgClNPs) were fabricated using Onopordum acanthium L extract and their apoptotic and cytotoxicity properties on breast cancer MDA_MB232 and normal HEK293 cell lines were also evaluated. AgClNPs formation was determined by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) pro le. Effect of fabricated AgClNPs on MDA_MB232 and HEK293 cells viability was performed using colorimetric MTT assay. Alterations in the mRNA expression levels of CAD and Bax genes in MDA-MB-232 cells were done using quantitative real-time reverse transcription-PCR (qRT-PCR) method. Subsequently, apoptotic properties were determined using ow cytometry and uorescence microscopy studies. MTT results investigated that AgCLNPs have a signi cant dose-dependent lethal activity on MDA_MB232 compared to HEK293 cell lines. Quantitative real-time reverse transcription-PCR (qRT-PCR) results have also shown that AgCLNPs could up-regulate the apoptotic Bax and CAD gene expressions in the MDA_MB232 cells. Additionally, apoptotic assessment was performed by cell cycle analysis, annexin V/PI test, Hoescht 33258 dye, acridine orange and ethidium bromide (AO/EB) staining along with the detection of the reactive oxygen species (ROS) generation. Our results suggest that novel silver chloride nanoparticles fabricated by Onopordum acanthium L extract can display some promising cytotoxic properties through inducing apoptosis pathway.

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“…26 Khayati and co-workers conveyed that Ag-AgCl-NPs indicated meaningful anticancer activity against MDA-MB-468 cell lines. 20 However, the colloidal form of Ag and AgCl nanoparticles have some hitches, for instance easy aggregation, negligible dispersion, fast release, and poor storage constancy which restrict their feasible application and their antibacterial activity. 19 To solve these problems and/or enhance the antibacterial activities, various organic/inorganic support such as natural polymers (cellulose, starch), 27 silica, 21 carbon materials (graphite), 28 metal−organic frameworks (MOF), 29 and titanium dioxide 30 have been used to stabilize silver nanoparticles.…”

Section: ■ Introductionmentioning

confidence: 99%

“…With the gentle progress of the population’s environmental awareness and living standards, research for the development of biomaterials is increasing. − For this purpose, many efforts pertinent to antibacterial medications and also antibiotic resistance of bacteria have been investigated , and inorganic nanoparticles, including Au, Ti, , Cu, MgO, ZnO, and Ag − have been used to reduce the risk of public health. In this regard, silver and its derivatives (AgCl NPs and Ag-AgCl NPs) due to having thermal stability, good pollution resistance, potent antibactericidal ability, health, and environmental properties, have been attractive for use in widespread applications including catalysis, optics, nanoelectronics, treatment of various diseases and medical biomedicine .…”

Section: Introductionmentioning

confidence: 99%

“…For example, a green method has been reported using Sasa Borealis extract to synthesize Ag-AgCl-NPs and assessed their anticancer properties versus the ventral adenocarcinoma cells . Khayati and co-workers conveyed that Ag-AgCl-NPs indicated meaningful anticancer activity against MDA-MB-468 cell lines . However, the colloidal form of Ag and AgCl nanoparticles have some hitches, for instance easy aggregation, negligible dispersion, fast release, and poor storage constancy which restrict their feasible application and their antibacterial activity .…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of MCC/IL/Ag-AgCl NPs by Cellulose-Based Nanocomposite for Medical Antibiofilm Applications

Hasanzadeh

Shojaei

Gholipour

et al. 2023

Ind. Eng. Chem. Res.

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In biomedical applications, biocompatible and nontoxic compounds are the most critical types of cytotoxic effects. In this work, the nanobiomaterial MCC/IL/Ag-AgCl NPs were fabricated by immobilizing Ag-AgCl nanoparticles on the surface of organic biopolymer [microcrystalline cellulose (MCC)] without using any reducing agent. The physical and chemical properties of nanobiomaterial were characterized by techniques including transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) analysis, X-ray diffraction (XRD), FOurier transform infrared (FT-IR) spectroscopy, and atomic absorption spectroscopy (AAS). The in vitro antibacterial activity of MCC/IL/Ag-AgCl NPs was investigated on methicillin-resistant Staphylococcus aureus (ATCC-43300) and multidrug-resistant Pseudomonas aeruginosa (ATCC-27853). The results such as MIC and MBC illustrated that the antimicrobial effect of MCC/IL/Ag-AgCl NPs against Pseudomonas aeruginosa (1−2 μg/mL of MIC and MBC value) was higher than that of Staphylococcus aureus (4 μg/mL of MIC and MBC value) and also by using the microtiter plate method, the antibiofilm effect of MCC/IL/Ag-AgClNPs against Pseudomonas aeruginosa was remarkable in a variety of low concentrations (32, 16, 8, 4, 2, and 1 μg/mL). Besides, the cytotoxicity study (cell death induction) by MCC/IL/Ag-AgCl NPs in Caco-2 cells revealed low toxic effects in different doses of MCC/IL/Ag-AgCl NPs up to 400 ppm for Ag-AgCl nanoparticles and MCC/IL support, which were further confirmed in biomedical products. It is expected that using these biocompatible compounds can inhibit the biofilm formation of bacteria on surfaces and equipment, to reduce nosocomial infections, in hospital environments, especially in the intensive care unit, and surgery unit/room.

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Biosynthesis of Silver–Silver Chloride Nanoparticles Using Fruit Extract of Levisticum Officinale: Characterization and Anticancer Activity Against MDA-MB-468 Cell Lines

Cited by 11 publications

References 32 publications

Facile Green Synthesis of Ag/Agcl Nanocomposite Using Durian Shell Extract and Its Activity Against Methicillin-Resistant Staphylococcus Aureus;

Facile Green Synthesis of Ag/Agcl Nanocomposite Using Durian Shell Extract and Its Activity Against Methicillin-Resistant Staphylococcus Aureus;

An Eco-friendly Fabrication of Silver Chloride Nanoparticles (AgClNPs) using Onopordum acanthium L. extract Induces Apoptosis in Breast Cancer MDA-MB-232 Cells

Biosynthesis of MCC/IL/Ag-AgCl NPs by Cellulose-Based Nanocomposite for Medical Antibiofilm Applications

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