Biosynthesis of tannase from cashew testa using Aspergillus niger MTCC5889 by solid state fermentation

Viswanath, Vinod; Leo, Vincent Vineeth; Prabha, Sabna S.; Prabhakumari, C.; Potty, V. P.; Jisha, M. S.

doi:10.1007/s13197-015-1858-4

Cited by 15 publications

(8 citation statements)

References 27 publications

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“…Figure 4 explains the process of electrophoresis in polyacrylamide gel with SDS for standard proteins and pure enzyme, the results were different between researchers, it agreed with the findings of [20] that the molecular weight for tannase product from Aspergillus niger reached to 89.9 KDa estimated in an SDS-PAGE, while molecular weight was 80 KDa for tannase product from Aspergillus niger in SDS-PAGE [16].…”

Section: Molecular Weightsupporting

confidence: 62%

Purification and characterization of tannase from the local isolate of Aspergillus niger

2019

jabb

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Fungi of genus Aspergillus are active producers of extracellular tannase, and characteristics of enzyme production by local Aspergillus species have been well studied. The aim of this experimental study is isolation and partial purification of tannase enzyme from the Aspergillus niger fungus species. The purification was achieved by applying respectively ammonium sulfate (50-70%), dialysis with a specific activity 1227.08 (unit/mg protein), then passed the enzymatic extract through superdex 200 column in the gel filtration chromatography by using ÄKTA Pure 25 apparatus, the activity and the specific activity reached to 525.12 (unit/ml) and 2917.33 (unit/mg protein) respectively, with purification fold of 11.629 and yield 18.40%. One peak was obtained from an enzyme purity, which diagnosed by the absence of denaturation substances for protein SDS. Characteristics of pure enzyme showed that the molecular weight of the pure enzyme was determined, and it was found that the weight of enzyme was 95.49 KDa by using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, the enzyme was active in pH range 3 to 6 and temperature of 20 to 50°C. The optimum pH and the temperature for tannase activity were recorded to be pH 5 and 35°C, respectively. Moreover, the tannase activity was inhibited by Cu, Fe and Hg ions while increased by Mg and K ions when added at 1 and 5 mM.

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Section: Molecular Weightsupporting

confidence: 62%

Purification and characterization of tannase from the local isolate of Aspergillus niger

2019

jabb

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“…When the enzyme was placed under temperatures above 40°C, a decrease in tannase activity was observed. Some tannase characterisation studies using genus Aspergillus have indicated that A. niger MTCC5889 under SSF and using cashew bagasse as a substrate yielded optimum temperature and pH level for tannase production at 40°C and 5.5, respectively (Viswanath et al, 2015). Similarly, using A. niger, Ni et al (2015) demonstrated maximum tannase activity at a pH level of 5.5 and with an optimum temperature at 50°C.…”

Section: Resultsmentioning

confidence: 99%

“…2b) showed the enzyme to be 66.5 kDa. Aspergillus niger MTCC5889 tannase analysed by SDS-PAGE had an 89.9 kDa molecular weight (Viswanath et al, 2015). The recombinant (rAoTanA) and native (AoTanA) enzymes produced by Aspergillus oryzae and expressed by Pichia pastoris yielded molecular weights of 45-80 and 45-75 kDa, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Inoculum was prepared by suspending spores in Agar Czapek tubes containing 80% tween solution with distilled water until obtaining 10 7 spores per gram per count at a Neubauer chamber. Tannic acid solution was added to nutrient solution prepared in sodium phosphate buffer (10 mM, pH 5.5) containing 0.5% yeast extract and 1% dextrose (Viswanath et al, 2015). Initial moisture was determined according to Adolfo Lutz Institute norms (Zenebon et al, 2008).…”

Section: Tannase Production By Solid-state Fermentation (Ssf)mentioning

confidence: 99%

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Tannase from Aspergillus melleus improves the antioxidant activity of green tea: purification and biochemical characterisation

Liu

Costa

Freitas

et al. 2016

Int J of Food Sci Tech

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Summary Tannase is an inducible enzyme used extensively in food, feed, pharmaceutical and chemical industries. In this study, tannase production and its biochemical properties were evaluated. From 42 Aspergillus strains analysed for potential tannase selection, Aspergillus melleus yielded the best results. Production was analysed using a complete factorial planning of 2³. Maximum activity (452.55 U mL−1) was obtained in the optimal conditions of substrate (5.0 g), initial moisture (60%), tannic acid (2%) and 48 h of fermentation. The molecular weight of the purified enzyme was estimated as 69.52 kDa; its optimum temperature and pH were 40 °C and 5.5, respectively. Regarding the chemical effectors used, tannase was inhibited by ZnCl2, ZnSO4, Triton X‐100 and SDS. The addition of tannase to green tea improved its antioxidant potential by approximately 85% when compared to the control. The present results suggest that tannase may be used as an adjuvant to increase the antioxidant potential of green tea.

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“…Therefore, the previous studies have used the yield or activity of the target product as the sole index to evaluate or optimize SSF systems, such as those used for l (+)-lactic acid production from sugarcane bagasse [ 27 ], ethanol production from rice straw and husk [ 28 ], and rapeseed peptide production from rapeseed meal [ 29 ]. The same problem of complicated microbial biomass determination is also encountered in tannase SSF, because high amounts of agricultural byproducts, for example, castor bean residues [ 12 ], cashew testa [ 30 ], coffee husk [ 31 ], and tea stalk [ 26 ], are used as solid media to produce tannase. The previous studies have optimized fermentation conditions on the basis of a single evaluation index, including tannase activity, and ignored the effect of cell growth on tannase synthesis.…”

Section: Discussionmentioning

confidence: 99%

Novel optimization strategy for tannase production through a modified solid-state fermentation system

Zhang

et al. 2018

Biotechnol Biofuels

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BackgroundHigh amounts of insoluble substrates exist in the traditional solid-state fermentation (SSF) system. The presence of these substrates complicates the determination of microbial biomass. Thus, enzyme activity is used as the sole index for the optimization of the traditional SSF system, and the relationship between microbial growth and enzyme synthesis is always ignored. This study was conducted to address this deficiency. All soluble nutrients from tea stalk were extracted using water. The aqueous extract was then mixed with polyurethane sponge to establish a modified SSF system, which was then used to conduct tannase production. With this system, biomass, enzyme activity, and enzyme productivity could be measured rationally and accurately. Thus, the association between biomass and enzyme activity could be easily identified, and the shortcomings of traditional SSF could be addressed.ResultsDifferent carbon and nitrogen sources exerted different effects on microbial growth and enzyme production. Single-factor experiments showed that glucose and yeast extract greatly improved microbial biomass accumulation and that tannin and (NH4)2SO4 efficiently promoted enzyme productivity. Then, these four factors were optimized through response surface methodology. Tannase activity reached 19.22 U/gds when the added amounts of tannin, glucose, (NH4)2SO4, and yeast extract were 7.49, 8.11, 9.26, and 2.25%, respectively. Tannase activity under the optimized process conditions was 6.36 times higher than that under the initial process conditions. The optimized parameters were directly applied to the traditional tea stalk SSF system. Tannase activity reached 245 U/gds, which is 2.9 times higher than our previously reported value.ConclusionsIn this study, a modified SSF system was established to address the shortcomings of the traditional SSF system. Analysis revealed that enzymatic activity and microbial biomass are closely related, and different carbon and nitrogen sources have different effects on microbial growth and enzyme production. The maximal tannase activity was obtained under the optimal combination of nutrient sources that enhances cell growth and tannase accumulation. Moreover, tannase production through the traditional tea stalk SSF was markedly improved when the optimized parameters were applied. This work provides an innovative approach to bioproduction research through SSF.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1093-0) contains supplementary material, which is available to authorized users.

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Biosynthesis of tannase from cashew testa using Aspergillus niger MTCC5889 by solid state fermentation

Cited by 15 publications

References 27 publications

Purification and characterization of tannase from the local isolate of Aspergillus niger

Purification and characterization of tannase from the local isolate of Aspergillus niger

Tannase from Aspergillus melleus improves the antioxidant activity of green tea: purification and biochemical characterisation

Novel optimization strategy for tannase production through a modified solid-state fermentation system

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