Biosynthesis of UDP-4-keto-6-deoxyglucose and UDP-rhamnose in Pathogenic Fungi Magnaporthe grisea and Botryotinia fuckeliana

Abstract

Background: Rhamnose-containing glycans are involved in host-pathogen interactions. Results: UDP-rhamnose was identified in fungi; recombinant enzymes involved in its synthesis were characterized, and the genes involved are expressed in a tissue-specific manner. Conclusion: Fungi containing rhamnose likely utilize the UDP-rhamnose pathway. Significance: Understanding rhamnose pathways in fungi may provide new insight to fungus-host interaction.

Downregulation of a UDP-Arabinomutase Gene in Switchgrass (Panicum virgatum L.) Results in Increased Cell Wall Lignin While Reducing Arabinose-Glycans

“…The tillers were cut and divided into stem and leaf sections. A sample section was weighed, ground in liquid nitrogen, and washed as previously reported (Martinez et al, 2012) with slight modifications. Each 1 g sample was suspended in 10 mL 80% EtOH, vortexed for 2 min, then centrifuged (6,000 × g 5 min, 25°C).…”

Genetic alteration of UDP‐rhamnose metabolism in Botrytis cinerea leads to the accumulation of UDP‐KDG that adversely affects development and pathogenicity

“…Because of its great economic implications, B. cinerea has become a model system for the analysis of necrotrophic pathogens (Amselem et al ., ; Williamson et al ., ). The role of the B. cinerea cell wall has recently inspired interest because of the finding of polysaccharides containing L‐rhamnose (Rha) residues (Martinez et al ., ), a sugar residue commonly found in plant wall polysaccharides. Although a minor component, Rha was isolated from a B. cinerea glycan in EPS, which comprises galactose (Gal), Glc, Rha and mannose (Man).…”

Haloferax volcanii N-Glycosylation: Delineating the Pathway of dTDP-rhamnose Biosynthesis

“…In Bacteria, plants and fungi, rhamnose is a common component of the cell wall [15]–[17], and was also recently found in viruses [18]. At present, two pathways for synthesizing nucleotide-activated rhamnose are known.…”