Biosynthesis of uronamide sugars in<i>Pseudomonas aeruginosa</i>O6 and<i>Escherichia coli</i>O121 O antigens

King, Jerry D.; Vinogradov, Evgeny; Tran, Vanessa; Lam, Joseph S.

doi:10.1111/j.1462-2920.2010.02182.x

Cited by 15 publications

(12 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, under ammonia-limiting conditions, such as growth in nitrogen-free medium with alanine added, the activity of MMP1081 becomes dependent on the ammonia generated by the glutaminase activity of MMP1082 and funneled by MMP1083. It has been reported that effects of deletions in other glutaminase systems are typically not detected when cells are grown in rich medium with sufficient ammonia (27) but become evident when cells are grown in minimal medium without ammonia addition. This was shown to be the case with the mmp1082 and mmp1083 deletion strains in this study.…”

Section: Resultsmentioning

confidence: 99%

Identification of Genes Involved in the Acetamidino Group Modification of the Flagellin N-Linked Glycan of Methanococcus maripaludis

Jones

Ding

et al. 2012

Journal of Bacteriology

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Identification of Genes Involved in the Acetamidino Group Modification of the Flagellin N-Linked Glycan of Methanococcus maripaludis

Jones

Ding

et al. 2012

Journal of Bacteriology

View full text Add to dashboard Cite

“…A number of rare sugars are found in the OSA of P. aeruginosa LPS, e.g., l-FucNAc (a constituent of the O11 OSA), di- N -acetylated mannuronic acid ( D -ManNAc3NAcA, a constituent of the O5 OSA), and a uronamide sugar GalNAcAN (a constituent of O6 OSA). The metabolic pathways for the biosynthesis of the nucleotide-activated precursors for these rare sugars are only found in a few bacterial pathogens but not in humans (Kneidinger et al, 2003; Westman et al, 2008, 2009; King et al, 2010). The biosynthesis of UDP- L -FucNAc from the substrate UDP- D -GlcNAc, requires the products of three genes from the serotype O11 OSA cluster, namely, wbjB , wbjC , and wbjD .…”

Section: Genetics Of O-specific Antigen Biosynthesismentioning

confidence: 99%

“…Interestingly, functional homologs of these three genes were found in Escherichia coli O121. As well, homologs of these three genes were also found in Francisella tularensis (King et al, 2010); however, they have not been tested to determine whether they could complement knockout mutants of the P. aeruginosa genes to restore production of O6 LPS that contains GalNAcAN in its O-Ag unit. Therefore, P. aeruginosa and other bacterial pathogens likely acquired genes for the biosynthesis of the abovementioned UDP-sugars from common ancestors.…”

Section: Genetics Of O-specific Antigen Biosynthesismentioning

confidence: 99%

Genetic and Functional Diversity of Pseudomonas aeruginosa Lipopolysaccharide

et al. 2011

Self Cite

View full text Add to dashboard Cite

Lipopolysccharide (LPS) is an integral component of the Pseudomonas aeruginosa cell envelope, occupying the outer leaflet of the outer membrane in this Gram-negative opportunistic pathogen. It is important for bacterium–host interactions and has been shown to be a major virulence factor for this organism. Structurally, P. aeruginosa LPS is composed of three domains, namely, lipid A, core oligosaccharide, and the distal O antigen (O-Ag). Most P. aeruginosa strains produce two distinct forms of O-Ag, one a homopolymer of D-rhamnose that is a common polysaccharide antigen (CPA, formerly termed A band), and the other a heteropolymer of three to five distinct (and often unique dideoxy) sugars in its repeat units, known as O-specific antigen (OSA, formerly termed B band). Compositional differences in the O units among the OSA from different strains form the basis of the International Antigenic Typing Scheme for classification via serotyping of different strains of P. aeruginosa. The focus of this review is to provide state-of-the-art knowledge on the genetic and resultant functional diversity of LPS produced by P. aeruginosa. The underlying factors contributing to this diversity will be thoroughly discussed and presented in the context of its contributions to host–pathogen interactions and the control/prevention of infection.

show abstract

“…The amino acid sequence shares high homology (37% identity and 55% similarity) with the glutamine amidotransferase protein WbpS from P. aeruginosa. In P. aeruginosa, WbpS catalyzes the biosynthesis of uronamides by transfer of ammonia to the carboxylate moiety of the corresponding uronic acid and it is therefore required for lipopolysaccharide O-antigen biosynthesis (King et al 2010). It is well known that lipopolysaccharides are involved in colony morphology (rugose vs. smooth) in many bacteria.…”

Section: Other Genes That Affect the Colony Morphology And Cells Automentioning

confidence: 99%

EmbRS a new two‐component system that inhibits biofilm formation and saves Rubrivivax gelatinosus from sinking

et al. 2013

View full text Add to dashboard Cite

Photosynthetic bacteria can switch from planktonic lifestyle to phototrophic biofilm in mats in response to environmental changes. The mechanisms of phototrophic biofilm formation are, however, not characterized. Herein, we report a two-component system EmbRS that controls the biofilm formation in a photosynthetic member of the Burkholderiales order, the purple bacterium Rubrivivax gelatinosus. EmbRS inactivation results in cells that form conspicuous bacterial veils and fast-sinking aggregates in liquid. Biofilm analyses indicated that EmbRS represses the production of an extracellular matrix and biofilm formation. Mapping of transposon mutants that partially or completely restore the wild-type (WT) phenotype allowed the identification of two gene clusters involved in polysaccharide synthesis, one fully conserved only in Thauera sp., a floc-forming wastewater bacterium. A second two-component system BmfRS and a putative diguanylate cyclase BdcA were also identified in this screen suggesting their involvement in biofilm formation in this bacterium. The role of polysaccharides in sinking of microorganisms and organic matter, as well as the importance and the evolution of such regulatory system in phototrophic microorganisms are discussed.

show abstract

Biosynthesis of uronamide sugars inPseudomonas aeruginosaO6 andEscherichia coliO121 O antigens

Cited by 15 publications

References 71 publications

Identification of Genes Involved in the Acetamidino Group Modification of the Flagellin N-Linked Glycan of Methanococcus maripaludis

Identification of Genes Involved in the Acetamidino Group Modification of the Flagellin N-Linked Glycan of Methanococcus maripaludis

Genetic and Functional Diversity of Pseudomonas aeruginosa Lipopolysaccharide

EmbRS a new two‐component system that inhibits biofilm formation and saves Rubrivivax gelatinosus from sinking

Contact Info

Product

Resources

About