Biosynthesis of zinc oxide nanoparticles using Phoenix dactylifera and their effect on biomass and phytochemical compounds in Juniperus procera

Abstract

Biosynthesized nanoparticles have played vital role recently, as suggested to be alternative to physical and chemical methods. In this study, biosynthesis of zinc oxide nanoparticles (ZnO NPs) were carried out using leaf extracts of Phoenix dactylifera L. and Zinc nitrate. The effect of ZnO nanoparticles on biomass and biochemical parameters was investigated. Biosynthesized ZnO nanostructure was characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), UV–visible spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Which resulted in spherical shape with size ranging between 16 to 35 nm of Biosynthesized ZnO nanoparticles and UV absorption beak at 370.5 nm with clear peaks of functional groups. The impact of different concentrations (0.0 mg/L, 80 mg/L and 160 mg/L) of biosynthesized ZnO nanoparticles on biomass and bioactive compounds production of Juniperus procera in vitro was investigated. The results showed that, biosynthesized ZnO NPs (80 mg/L and 160 mg/L) concentrations were boosted the growth of J. Procera with significantly compared to non-treated plants in vitro. The highest concentration (160 mg/L) of ZnO NPs was enhanced the growth of plant at beginning period, one month later shoots became yellow and callus turned to be brownish. Moreover, the influence of ZnO NPs on phytochemical compounds in callus of Juniperus procera was examined using GC–MS analysis. The differences among treatments were recoded. Overall, zinc oxide nanoparticles substantially improved the growth of shoots and callus with increasing of biochemical parameters such as chlorophyll a, total phenolic and flavonoids contents, besides the total protein and, SOD, CAT and APX activity. ZnO NPs might be induced some phytochemical compounds as well as inhibit.

Anticancer activities of Saudi Juniperus procera extracts and their effects on the regulatory mechanisms in controlling growth of human cancerous cells

“…procera are plentiful in phenolics and avonoids (Table 1). Similar ndings have been reported by 22,23,24 .…”

Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro

“…Moreover, it has been reported that ferruginol inhibits the growth of cancer cells ( González et al, 2014 ). Recently, we detected ferruginol in the different parts of J. procera using GC-MS, DART-MS, and HPLC ( Salih et al, 2021a , b ; Figure 8A ), and it is a dominant compound in different parts of this plant. This study separated ferruginol and identified it using HPLC, with ferruginol standard ( Figures 8B,C ).…”

“…The HPLC Agilent Technologies System controlled by software (G 4226A) with the column SB-C18 (1.8 μm, 4.6 × 150 mm) was used for chromatographic analysis of targeted compounds. For separation and quantification of the bioactive compounds such as gallic acid, hesperidin, quercetin, tannic acid, coumarin, and rutin; specific standards, mobile phases, wavelengths, injection volume, and flow rate were used for each compound following Nour et al’s (2013) and Salih et al’s (2021b) methods. The identification of these compounds in the callus samples was possible because their retention times spiked with the specific standard of each compound under similar conditions ( Figure 1 ).…”

Biologically synthesized iron nanoparticles (FeNPs) from Phoenix dactylifera have anti-bacterial activities

“…Phoenix dactylifera has been previously used for the synthesis of several other metallic nanoparticles. Such as Muhammad et al 26 synthesized silver nanoparticles by using leave extract of Phoenix dactylifera and Barani et al 27 also used leave extract of Phoenix dactylifera to synthesize zinc oxide nanoparticles. Thus, in this study, Phoenix dactylifera was used as a source to produce FeNPs.…”