Biosynthetic, biomimetic, and self-assembled vascularized Organ-on-a-Chip systems

Fritschen, Anna; Blaeser, Andreas

doi:10.1016/j.biomaterials.2020.120556

Cited by 40 publications

(41 citation statements)

References 158 publications

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“…Similarly, there is increasing investigation using artificially assembled microfluidic approaches that enable the cocultivation with capillary-like structures, as well as exposure to immune cells, with the advantage of also presenting exposure to “blood” flow. 111 , 112 To date, there are reports of airway microfluidic cultures, cocultivated with vascular and immune cells, for posterior exposure to pathogens, including SARS-CoV-2. 38 , 102 − 104 The microfluidic upside is that the blood-like tissue is pumped throughout the culture system, using a fluid flow, simulating the in vivo situation.…”

Section: Airway and Lung Organoid Modelsmentioning

confidence: 99%

“… 38 , 102 − 104 The microfluidic upside is that the blood-like tissue is pumped throughout the culture system, using a fluid flow, simulating the in vivo situation. 111 , 112 There are reports of the establishment of distal airway microfluidic models, or specifically alveolar cultures, cocultivated with microvasculature 102 , 104 and immune cells 102 − 104 on a chip, for further SARS-CoV-2 exposure and investigation. The results indicate the important involvement of immune responses through the alveolar barrier, showing that the microfluidic system resembles in vivo situations and could be valid for research that requires interaction to blood and immune cells.…”

Section: Airway and Lung Organoid Modelsmentioning

confidence: 99%

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Airway and Alveoli Organoids as Valuable Research Tools in COVID-19

Oliveira

Síbio

Costa

et al. 2021

ACS Biomater. Sci. Eng.

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The coronavirus disease 2019 (COVID-19), caused by the novel coronavirus, SARS-CoV-2, affects tissues from different body systems but mostly the respiratory system, and the damage evoked in the lungs may occasionally result in severe respiratory complications and eventually lead to death. Studies of human respiratory infections have been limited by the scarcity of functional models that mimic in vivo physiology and pathophysiology. In the last decades, organoid models have emerged as potential research tools due to the possibility of reproducing in vivo tissue in culture. Despite being studied for over one year, there is still no effective treatment against COVID-19, and investigations using pulmonary tissue and possible therapeutics are still very limited. Thus, human lung organoids can provide robust support to simulate SARS-CoV-2 infection and replication and aid in a better understanding of their effects in human tissue. The present review describes methodological aspects of different protocols to develop airway and alveoli organoids, which have a promising perspective to further investigate COVID-19.

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Section: Airway and Lung Organoid Modelsmentioning

confidence: 99%

Section: Airway and Lung Organoid Modelsmentioning

confidence: 99%

Airway and Alveoli Organoids as Valuable Research Tools in COVID-19

Oliveira

Síbio

Costa

et al. 2021

ACS Biomater. Sci. Eng.

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“…Bio-inspired or biomimetic design of biomaterials presents new possibilities for developing implantable devices with enhanced biocompatibility and novel functions [1][2][3][4][5][6][7][8]. No study has yet reported a potential application of biomimetic surface morphology, particularly at the nano-level, to endosseous implants for commercial use in the fields of dental and orthopedic surgery [9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Zirconia with Cactus-Inspired Meso-Scale Spikes and Nano-Trabeculae for Enhanced Bone Integration

Saruta

Ozawa

Okubo

et al. 2021

IJMS

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Biomimetic design provides novel opportunities for enhancing and functionalizing biomaterials. Here we created a zirconia surface with cactus-inspired meso-scale spikes and bone-inspired nano-scale trabecular architecture and examined its biological activity in bone generation and integration. Crisscrossing laser etching successfully engraved 60 μm wide, cactus-inspired spikes on yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) with 200–300 nm trabecular bone-inspired interwoven structures on the entire surface. The height of the spikes was varied from 20 to 80 μm for optimization. Average roughness (Sa) increased from 0.10 μm (polished smooth surface) to 18.14 μm (80 μm-high spikes), while the surface area increased by up to 4.43 times. The measured dimensions of the spikes almost perfectly correlated with their estimated dimensions (R2 = 0.998). The dimensional error of forming the architecture was 1% as a coefficient of variation. Bone marrow-derived osteoblasts were cultured on a polished surface and on meso- and nano-scale hybrid textured surfaces with different spike heights. The osteoblastic differentiation was significantly promoted on the hybrid-textured surfaces compared with the polished surface, and among them the hybrid-textured surface with 40 μm-high spikes showed unparalleled performance. In vivo bone-implant integration also peaked when the hybrid-textured surface had 40 μm-high spikes. The relationships between the spike height and measures of osteoblast differentiation and the strength of bone and implant integration were non-linear. The controllable creation of meso- and nano-scale hybrid biomimetic surfaces established in this study may provide a novel technological platform and design strategy for future development of biomaterial surfaces to improve bone integration and regeneration.

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“…While polydimethylsiloxane (PDMS) is the most commonly preferred material due to its high biocompatibility, transparency, and oxygen permeability, high level adsorption of proteins on the surface of PDMS have been reported by multiple studies, which results in the given drug or stimulating substance to not to fully interact with the cells within the chip [88]. In addition, the interaction between the cells and ECM is crucial for organ function as well as pathogen colonization; however, synthetic biomaterials usually possess mechanical properties different to native ECM [89]. While patient-specificity is a remarkable aspect of organ chips, patient tissue cells are usually limited in number or exhibit low proliferation, and their collection may require invasive techniques.…”

Section: Limitationsmentioning

confidence: 99%

Refining Host-Pathogen Interactions: Organ-on-Chip Side of the Coin

Baddal

Marrazzo

2021

Pathogens

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Bioinspired organ-level in vitro platforms that recapitulate human organ physiology and organ-specific responses have emerged as effective technologies for infectious disease research, drug discovery, and personalized medicine. A major challenge in tissue engineering for infectious diseases has been the reconstruction of the dynamic 3D microenvironment reflecting the architectural and functional complexity of the human body in order to more accurately model the initiation and progression of host–microbe interactions. By bridging the gap between in vitro experimental models and human pathophysiology and providing alternatives for animal models, organ-on-chip microfluidic devices have so far been implemented in multiple research areas, contributing to major advances in the field. Given the emergence of the recent pandemic, plug-and-play organ chips may hold the key for tackling an unmet clinical need in the development of effective therapeutic strategies. In this review, latest studies harnessing organ-on-chip platforms to unravel host–pathogen interactions are presented to highlight the prospects for the microfluidic technology in infectious diseases research.

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Biosynthetic, biomimetic, and self-assembled vascularized Organ-on-a-Chip systems

Cited by 40 publications

References 158 publications

Airway and Alveoli Organoids as Valuable Research Tools in COVID-19

Airway and Alveoli Organoids as Valuable Research Tools in COVID-19

Biomimetic Zirconia with Cactus-Inspired Meso-Scale Spikes and Nano-Trabeculae for Enhanced Bone Integration

Refining Host-Pathogen Interactions: Organ-on-Chip Side of the Coin

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