Biotech rice: Current developments and future detection challenges in food and feed chain

Fraiture, Marie‐Alice; Roosens, Nancy; Taverniers, Isabel; Loose, Marc De; Deforce, Dieter; Herman, Philippe

doi:10.1016/j.tifs.2016.03.011

Cited by 36 publications

(20 citation statements)

References 102 publications

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“…In DNA-based methods, molecular laboratory protocols are the proper choice for the screening and quantification of the GM constructs (FRAITURE et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Development of an efficient element-specific PCR method for the detection of genetically modified rice, soy & corn

Sepahiann¹,

Noormohammadi²,

Sheidai³

et al. 2019

Genetika

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Sepahian N., Z. Noormohammadi, M. Sheidai, H.-R. Zamanizadeh (2019): Development of an efficient element-specific PCR method for the detection of genetically modified rice, Vol 51, No.3,[895][896][897][898][899][900][901][902][903][904][905] With the ever-increasing number of GM crops introduced to the market, it is required to respect customers' freedom of choice and their perception toward GMOs. According to obligatory demand by food and drug administration for GM testing on raw and processed materials, efficient and robust analytical approaches are needed for screening and identification of GM crops. This article describes the development and applicability of a PCR-based GMO testing of the most frequent constructs used in transgenic soy, corn and rice varieties which include CaMV35S promoter and the NOS terminator and also plant endogenous sequences Lectin, Zein, SPS as internal controls. A total of 2866 different sample inputs collected during the year 2015 to 2017 including GM and non-GM materials have been tested. Results obtained from qualitative PCR test on rice, maize and soybean-containing food samples showed that in total, 1.59% of maize samples and 896 GENETIKA, Vol. 51, No3, 895-905, 2019 7.53% of soya samples were GM positive and no GM positive rice sample was detected. The presented method showed high specificity and sensitivity offering an absolute limit of detection of 0.25% GM construct in the samples and sufficient reproducibility of the method was proved. This method fits the purpose of GMO testing laboratories due to its convenience and robustness.

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“…In DNA-based methods, molecular laboratory protocols are the proper choice for the screening and quantification of the GM constructs (FRAITURE et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Development of an efficient element-specific PCR method for the detection of genetically modified rice, soy & corn

Sepahiann¹,

Noormohammadi²,

Sheidai³

et al. 2019

Genetika

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“…Rice provides more than 20% of the total caloric intake for half of the world’s population [ 1 ]. Under irrigated environments, bacterial blight (BB) represents one of the major biotic constraints to sustain rice production.…”

Section: Introductionmentioning

confidence: 99%

Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae

et al. 2018

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Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the major diseases that impact rice production in Asia. The bacteria use transcription activator-like effectors (TALEs) to hijack the host transcription machinery and activate key susceptibility (S) genes, specifically members of the SWEET sucrose uniporters through the recognition of effector-binding element (EBEs) in the promoter regions. However, natural variations in the EBEs that alter the binding affinity of TALEs usually prevent sufficient induction of SWEET genes, leading to resistance phenotypes. In this study, we identified candidate resistance alleles by mining a rice diversity panel for mutations in the promoter of OsSWEET13 and OsSWEET14, which are direct targets of three major TALEs PthXo2, PthXo3 and AvrXa7. We found natural variations at the EBE of both genes, which appeared to have emerged independently in at least three rice subspecies. For OsSWEET13, a 2-bp deletion at the 5th and 6th positions of the EBE, and a substitution at the 17th position appear to be sufficient to prevent activation by PthXo2. Similarly, a single nucleotide substitution at position 10 compromised the induction of OsSWEET14 by AvrXa7. These findings might increase our opportunities to reduce pathogen virulence by preventing the induction of SWEET transporters. Pyramiding variants along with other resistance genes may provide durable and broad-spectrum resistance to the disease.

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“…Compared to traditional method, genetic engineering can be used to rise and stabilize yield, herbicide tolerance, disease and insect resistance, nutritional improvements and withstanding to abiotic stresses. The genetically modified (GM) rice was developed for the first time by plant transformation methods in 1988 [2,3]. The aims of GM rice production were improvement of quality and reduction in pesticides or herbicides used in the fields, that could not be achieved through other breeding methods [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Cauliflower mosaic virus (CaMV) 35S promoter (P-35S) and nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens are the most common transgenic elements that can be targeted for GMO screening [2,[14][15][16]. These genetic elements are frequently used to indicate whether the analyzed sample contains GM ingredient or not.…”

Section: Introductionmentioning

confidence: 99%

A simple and accurate PCR method for detection of genetically modified rice

Safaei

Aghaee

Khaniki

et al. 2019

J Environ Health Sci Engineer

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Background Legislation regulating for labeling and use of genetically modified (GM) crops are increased considerably worldwide in order to health and safety assurance of consumers. For this purpose, a polymerase chain reaction (PCR) method has been developed for detection of GM rice in people's food diet. Methods In this study, eighty-one non-labeled rice samples were collected randomly from different market sites of Tehran, Iran. In order to analysis, rice genomic DNA was extracted using MBST DNA extraction kit and subsequently, sucrose phosphate synthase (SPS) gene was used to confirm the quality of extracted DNA. Then, cauliflower mosaic virus (CaMV) 35S promoter and Agrobacterium nopaline synthase (NOS) terminator were selected as screening targets for detection of GM rice sequences by PCR. Results According to our results, 2 out of 81 (2.4%) samples tested were positive for CaMV 35S promoter while no positive result was detected for NOS terminator. Conclusion The obtained data indicated that this method is capable to identify the GM rice varieties. Furthermore, it can demonstrate the possibility of the presence of GM rice in Tehran's market, thus putting emphasis on the requirement for developing a precise approach to evaluate this product.

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Biotech rice: Current developments and future detection challenges in food and feed chain

Cited by 36 publications

References 102 publications

Development of an efficient element-specific PCR method for the detection of genetically modified rice, soy & corn

Development of an efficient element-specific PCR method for the detection of genetically modified rice, soy & corn

Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae

A simple and accurate PCR method for detection of genetically modified rice

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