Biotechnological advances in guava (Psidium guajava L.): recent developments and prospects for further research

Manoj, K.; Asthana, Pooja; Jaiswal, Vivek; Jaiswal, Uma

doi:10.1007/s00468-009-0384-2

Cited by 125 publications

(58 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MS medium supplemented with 8.88 μM BAP was most suitable for multiple shoot induction and it induced 100 % bud break (Table 1). The effectiveness of BAP on multiple shoot induction has also been reported in a number of plants (Rai et al 2010;Phulwaria et al 2012a, b).…”

Section: Establishment Of Culturesmentioning

confidence: 71%

“…The treatment of explants with chilled, sterile antioxidant solution checked excessive phenolic exudation and browning of tissue and cultures (Phulwaria et al 2012a, b). It has been observed in woody plants that antioxidant solutions may promote growth and development of the shoots particularly at the stage of culture establishment (Shekhawat et al 1993;Sanjaya et al 2006;Rai et al 2010). Explants were cultured on 0.8 % agar-gelled MS medium containing BAP or Kn for shoot bud induction.…”

Section: Establishment Of Culturesmentioning

confidence: 99%

See 1 more Smart Citation

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Vibha

Shekhawat

Mehandru

et al. 2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

An efficient and improved method for in vitro propagation of mature tree of Dalbergia sissoo, an ecologically and commercially important timber yielding species, has been developed through axillary shoot proliferation. Bud breaking occurred from nodal shoot segments derived from rejuvenated shoots produced during early spring from a 20-25-year-old lopped tree, on MS medium containing 8.88 μM benzylaminopurine (BAP). Multiple shoots differentiated (20-21shoots/node) on re-culture of explants on halfstrength agar gelled amended MS medium with a combination of 2.22 μM of BAP and 0.002 μM of thidiazuron (TDZ) with 1.0 mM each of Ca(NO 3 ) 2 , K 2 SO 4 , KCl, and NH 4 (SO 4 ) 2 . The maximum shoot multiplication (29-30 shoots/node) was achieved on subculturing in the above mentioned but liquid medium. Furthermore, the problem of shoot tip necrosis and defoliation observed on solid medium were overcome by the use of liquid medium. Ex vitro rooting was achieved on soilrite after basal treatment of microshoots with 984 μM of indole-3-butyric acid (IBA) for 2 min. About 90 % microshoots were rooted on soilrite within 2-3 weeks under the greenhouse conditions. From 20 nodal shoot segments, about 435 hardened plants were acclimatized and transplanted. This is the first report for rapid in vitro propagation of mature trees of D. sissoo on liquid medium followed by ex vitro rooting.

show abstract

Section: Establishment Of Culturesmentioning

confidence: 71%

Section: Establishment Of Culturesmentioning

confidence: 99%

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Vibha

Shekhawat

Mehandru

et al. 2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…Woody nodal segment was not suitable for culture establishment owing to high phenolic exudation. Phenolic exudation is one the major problem in culture establishment when explants taken from woody tissues (Rai et al 2010). Furthermore, old branches shoots carry recalcitrant microbes in the tissues and these affect the process of surface sterilization and subsequent culture, behavior and growth of shoots (Bonga et al 2010).…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of commercially cultivated Henna (Lawsonia inermis) using nodal explants

Ram

Shekhawat

2011

Physiol Mol Biol Plants

View full text Add to dashboard Cite

“…Somatic embryogenesis is the main pathway for many plant species regeneration (Kirkorian 2000). Somatic embryogenesis provides a valuable tool to increase the efficiency of genetic improvement of many economically important crops (Rai et al 2010). Maturation deficiency of somatic embryos and low rate of transformation, development to plantlets are major problems of somatic embryo regeneration in many species of alfalfa.…”

Section: Introductionmentioning

confidence: 99%

Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Amini

Deljou

Nabiabad

2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Developmental deficiency of somatic embryos and regeneration to plantlets, especially in the case of transformation, are major problems of somatic embryo regeneration in alfalfa. One of the ways to overcome these problems is the use of natural plant regulators and nutrients in the culture medium of somatic embryos. For investigating the influence of Cuscuta campestris extract on the efficiency of plant regeneration and transformation, chimeric tissue type plasminogen activator was transferred to explants using Agrobacterium tumefaciens, and transgenic plants were recovered using medium supplemented with different concentration of the extract. Transgenic plants were analyzed by PCR and RT-PCR. Somatic embryos of Medicago sativa L. developed into plantlets at high frequency level (52 %) in the maturation medium supplemented with 50 mg 1 -1 C. campestris extract as compared to the medium without extract (26 %). Transformation efficiency was 29.3 and 15.2 % for medium supplemented with dodder extract and without the extract, respectively. HPLC and GC/MS analysis of the extract indicated high level of ABA and some compounds such as Phytol, which can affect the somatic embryo maturation. The antibacterial assay showed that the extract was effective against some strains of A. tumefaciens. These results have provided a scientific basis for using of C. campestris extract as a good natural source of antimicrobial agents and plant growth regulator as well, that can be used in tissue culture of transgenic plants.

show abstract

Biotechnological advances in guava (Psidium guajava L.): recent developments and prospects for further research

Cited by 125 publications

References 54 publications

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Micropropagation of commercially cultivated Henna (Lawsonia inermis) using nodal explants

Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Contact Info

Product

Resources

About