Biotechnological advances in tea (Camellia sinensis [L.] O. Kuntze): a review

Mukhopadhyay, Mainaak; Mondal, Tapan Kumar; Chand, Pradeep K.

doi:10.1007/s00299-015-1884-8

Cited by 115 publications

(56 citation statements)

References 223 publications

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“…As for cacao, a large number of metabolomics studies have been performed on coffee and tea identifying high contents of caffeine, quinate, and chlorogenic acid in the former [136][137][138] and catechins, terpenes, and caffeine in the latter [139][140][141]. Since there is also an increasing amount of transcriptomics data available for these species [142][143][144][145][146][147][148], it would appear likely that evaluating the dynamic behavior of transcripts related to caffeine biosynthesis in comparison to other unknown genes (and to the levels of the metabolites themselves) will greatly enhance our understanding as to how these pathways are controlled. One study of particular interest is the long read sequencing of the coffee bean transcriptome since this provided more and longer transcript variants specifically allowing the identification of a further 10 transcripts likely to encode key enzyme isoforms of caffeine biosynthesis [142].…”

Section: Caffeinementioning

confidence: 99%

The Integration of Metabolomics and Next-Generation Sequencing Data to Elucidate the Pathways of Natural Product Metabolism in Medicinal Plants

et al. 2018

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Plants have always been used as medicines since ancient times to treat diseases. The knowledge around the active components of herbal preparations has remained nevertheless fragmentary: the biosynthetic pathways of many secondary metabolites of pharmacological importance have been clarified only in a few species, while the chemodiversity present in many medicinal plants has remained largely unexplored. Despite the advancements of synthetic biology for production of medicinal compounds in heterologous hosts, the native plant species are often the most reliable and economic source for their production. It thus becomes fundamental to investigate the metabolic composition of medicinal plants to characterize their natural metabolic diversity and to define the biosynthetic routes of important compounds to develop strategies to further increase their content. We present here a number of case studies for selected classes of secondary metabolites and we review their health benefits and the historical developments in their structural elucidation and characterization of biosynthetic genes. We cover the cases of benzoisoquinoline and monoterpenoid indole alkaloids, cannabinoids, caffeine, ginsenosides, withanolides, artemisinin, and taxol; we show how the "early" biochemical or the more recent integrative approaches-based on omics-analyses-have helped to elucidate their metabolic pathways and cellular compartmentation. We also summarize how the knowledge generated about their biosynthesis has been used to develop metabolic engineering strategies in heterologous and native hosts. We conclude that following the advent of novel, high-throughput and cost-effective analytical technologies, the secondary metabolism of medicinal plants can now be examined under the lens of systems biology.

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Section: Caffeinementioning

confidence: 99%

The Integration of Metabolomics and Next-Generation Sequencing Data to Elucidate the Pathways of Natural Product Metabolism in Medicinal Plants

et al. 2018

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“…SE is an important method of plant regeneration, and several researchers have applied this technology to various plant species in recent years [21][22][23][24]. However, the process of SE is affected by many factors.…”

Section: Effect Of Plant Growth Regulators On Somatic Embryogenesismentioning

confidence: 99%

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

Yang

Guo

et al. 2018

Forests

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Research Highlights: In the current study, we established a method for plant regeneration via somatic embryogenesis (SE) in Koelreuteria paniculata Laxm. for the first time. Background and Objectives: K. paniculata is an important ornamental and medicinal plant in China. However, the plant has difficulty with asexual reproduction, which imposes a limitation on large-scale propagation. Materials and Methods: Embryogenic calluses were induced from stems of aseptic seedlings on induction media. The effects of different media types and concentrations of N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction were examined. Embryogenic calluses were then transferred to Driver-Kuniyuki Walnut (DKW) media containing NAA (0.1–0.2 mg L−1) or 2,4-D (0.5–2.0 mg L−1) to develop somatic embryos. Cotyledon embryos were cultured on DKW media containing NAA (0.1–0.2 mg L−1) until maturation, and were then transferred to 1/2 DKW medium supplemented with 1.0 mg L−1 indole-3-butyric acid (IBA) to produce complete plants. The effects of IBA and NAA on rhizogenesis were then examined by clonal culture. Results: The maximum callus induction frequency (80.25%) was obtained on DKW medium supplemented by 0.5 mg L−1 BA, 0.25 mg L−1 NAA, and 1.5 mg L−1 2,4-D. NAA had a more pronounced effect on somatic embryo growth than did 2,4-D, with a maximum SE frequency (54.75%) observed with 0.1 mg L−1 NAA added to DKW medium. For clonal culture, the highest rooting rate (52%) was observed on 1/4 DKW medium containing 1.5 mg L−1 IBA. Histology studies confirmed the presence of embryogenic calluses and somatic embryos in different stages. Conclusions: This protocol provides a novel method for large-scale propagation of K. paniculata, and creates opportunities for genetic engineering in this species.

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“…Molecular markers, based on DNA polymorphisms, are useful and powerful tools for genetic and breeding research. Numerous molecular markers have been successfully developed and applied in genetic and genomic research in tea plant, such as restriction fragment length polymorphisms (RFLPs), amplified fragment length polymorphisms (AFLPs), random amplification of polymorphic DNAs (RAPDs), cleaved amplified polymorphic sequences (CAPS), inter-simple sequence repeats (ISSRs), and simple sequence repeats (SSRs) [12,13]. With the rapid development of the highthroughput sequencing approaches, the third-generation single nucleotide polymorphism (SNP) and insertion/deletion (InDel) markers are gradually becoming the most widely used molecular markers, demonstrating a promising future in plant genetic and breeding research.…”

Section: Introductionmentioning

confidence: 99%

Characterization of genome-wide genetic variations between two varieties of tea plant (Camellia sinensis) and development of InDel markers for genetic research

Liu

Tong

et al. 2019

Preprint

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Background: Single nucleotide polymorphisms (SNPs) and insertions/deletions (InDels) are the major genetic variations and are distributed extensively across the whole plant genome. However, few studies of these variations have been conducted in the long-lived perennial tea plant. Results: In this study, we investigated the genome-wide genetic variations between Camellia sinensis var. sinensis ‘Shuchazao’ and Camellia sinensis var. assamica ‘Yunkang 10’, identified 7,511,731 SNPs and 255,218 InDels based on their whole genome sequences, and we subsequently analyzed their distinct types and distribution patterns. A total of 48 InDel markers that yielded polymorphic and unambiguous fragments were developed when screening six tea cultivars. These markers were further deployed on forty-six tea cultivars for transferability and genetic diversity analysis, exhibiting information with an average 4.02 of the number of alleles (Na) and 0.457 of polymorphism information content (PIC). The dendrogram showed that the phylogenetic relationships among these tea cultivars are highly consistent with their genetic backgrounds or original places. Interestingly, we observed that the catechin/caffeine contents between ‘Shuchazao’ and ‘Yunkang 10’ were significantly different, and a large number of SNPs/InDels were identified within catechin/caffeine biosynthesis-related genes. Conclusion: The identified genome-wide genetic variations and newly-developed InDel markers will provide a valuable resource for tea plant genetic and genomic studies, especially the SNPs/InDels within catechin/caffeine biosynthesis-related genes, which may serve as pivotal candidates for elucidating the molecular mechanism governing catechin/caffeine biosynthesis.

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Biotechnological advances in tea (Camellia sinensis [L.] O. Kuntze): a review

Cited by 115 publications

References 223 publications

The Integration of Metabolomics and Next-Generation Sequencing Data to Elucidate the Pathways of Natural Product Metabolism in Medicinal Plants

The Integration of Metabolomics and Next-Generation Sequencing Data to Elucidate the Pathways of Natural Product Metabolism in Medicinal Plants

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

Characterization of genome-wide genetic variations between two varieties of tea plant (Camellia sinensis) and development of InDel markers for genetic research

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