Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops

Villalobos-López, Miguel Ángel; Arroyo-Becerra, Analilia; Quintero-Jiménez, Anareli; Iturriaga, Gabriel

doi:10.3390/ijms231912053

Cited by 38 publications

(19 citation statements)

References 565 publications

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“…To cope with the adverse effects of global climate change, increasing population, and food insecurity, there is a dire need to develop and screen rice genotypes tolerant to water scarcity with high yield for sustainable rice production ( Villalobos-López et al., 2022 ). Plant growth-promoting bacteria (PGPB) can mitigate the adverse effects of drought on crops ( Barquero et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Mitigation of water scarcity with sustained growth of Rice by plant growth promoting bacteria

et al. 2023

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Climate change augments the risk to food security by inducing drought stress and a drastic decline in global rice production. Plant growth-promoting bacteria (PGPB) have been known to improve plant growth under drought stress. Here in the present study, we isolated, identified, and well-characterized eight drought-tolerant bacteria from the rice rhizosphere that are tolerant to 20% PEG-8000. These strains exhibited multiple plant growth-promoting traits, i.e., 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, exopolysaccharide production, phosphate (P)-solubilizing activity (51–356 µg ml-1), indole-3 acetic acid (IAA) production (14.3–46.2 µg ml-1), and production of organic acids (72–178 µg ml-1). Inoculation of bacterial consortium (Bacillus subtilis NM-2, Brucella haematophilum NM-4, and Bacillus cereus NM-6) significantly improved seedling growth and vigor index (1009.2-1100) as compared to non-inoculated stressed plants (630-957). Through rhizoscanning, efficiency of the consortium was validated by improved root parameters such as root length (17%), diameter, and surface area (18%) of all tested genotypes as compared with respective non-inoculated stressed treatments. Furthermore, the response of consortium inoculation on three rice genotypes was positively correlated with improved plant growth and drought stress ameliorating traits by the accumulation of osmoprotectant, i.e., proline (85.8%–122%), relative water content (51%), membrane stability index (64%), and production of antioxidant enzymes to reduce oxidative damage by reactive oxygen species. A decrease in temperature and improved chlorophyll content of inoculated plants were found using infrared thermal imaging and soil plant analyzer development (SPAD), respectively. The key supporting role of inoculation toward stress responses was validated using robust techniques like infrared thermal imaging and an infrared gas analyzer. Furthermore, principal component analysis depicts the contribution of inoculation on stress responses and yield of tested rice genotypes under water stress. The integration of drought-tolerant rice genotype (NIBGE-DT02) and potential bacterial strains, i.e., NM-2, NM-4, and NM-6, can serve as an effective bioinoculant to cope with water scarcity under current alarming issues related to food security in fluctuating climate.

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Section: Discussionmentioning

confidence: 99%

Mitigation of water scarcity with sustained growth of Rice by plant growth promoting bacteria

et al. 2023

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“…In this review, we will focus on drought, salinity, cold, and heat stress responses at the cellular and molecular levels. These are not the only abiotic conditions that will vary due to climate change, but they represent some of the major alterations that will result from it ( He et al., 2018 ; Villalobos-López et al., 2022 ). The stresses discussed in this review have a significant impact on the growth and development of plants, which is directly connected to crops’ yield and profitability ( Bita and Gerats, 2013 ; Bulgari et al., 2019 ).…”

Section: Cellular and Molecular Responses To Cope With The Main Abiot...mentioning

confidence: 99%

Improving abiotic stress tolerance of forage grasses – prospects of using genome editing

Sustek-Sánchez

Rognli²,

Rostoks³

et al. 2023

Front. Plant Sci.

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Due to an increase in the consumption of food, feed, and fuel and to meet global food security needs for the rapidly growing human population, there is a necessity to obtain high-yielding crops that can adapt to future climate changes. Currently, the main feed source used for ruminant livestock production is forage grasses. In temperate climate zones, perennial grasses grown for feed are widely distributed and tend to suffer under unfavorable environmental conditions. Genome editing has been shown to be an effective tool for the development of abiotic stress-resistant plants. The highly versatile CRISPR-Cas system enables increasingly complex modifications in genomes while maintaining precision and low off-target frequency mutations. In this review, we provide an overview of forage grass species that have been subjected to genome editing. We offer a perspective view on the generation of plants resilient to abiotic stresses. Due to the broad factors contributing to these stresses the review focuses on drought, salt, heat, and cold stresses. The application of new genomic techniques (e.g., CRISPR-Cas) allows addressing several challenges caused by climate change and abiotic stresses for developing forage grass cultivars with improved adaptation to the future climatic conditions. Genome editing will contribute towards developing safe and sustainable food systems.

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“…In this review, we will focus on drought, salinity, cold, and heat stress responses at the cellular and molecular levels. These are not the only abiotic conditions that will vary due to climate change, but they represent some of the major alterations that will result from it [35,36]. The stresses discussed in this review have a significant impact 3 of 33 on the growth and development of plants, which is directly connected to crops' yield and profitability [37,38].…”

Section: Cellular and Molecular Responses To Cope With The Main Abiot...mentioning

confidence: 99%

“…The toolkit of CRISPR-Cas applications has expanded to around twenty different techniques that allow diverse targeted modifications in the genome [36,150]. On one hand, Cas enzymes from different bacteria have been characterized and adopted for use.…”

Section: Gene Editing: a Tool For Developing Stress Resistant Forage ...mentioning

confidence: 99%

“…In these cases, the Cas9 nuclease is mutated in such a way that it acts as nickase, cutting only one strand of the targeted DNA. These strategies and the activation of homology-directed repair (HDR) instead of Non-Homologous End Joining (NHEJ), makes it possible to replace, insert or delete large sequences and even to generate chromosomal rearrangements [186,187].…”

Section: Gene Editing: a Tool For Developing Stress Resistant Forage ...mentioning

confidence: 99%

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Improving Abiotic Stress Tolerance of Forage Grasses – Prospects of Using Genome Editing

Sustek-Sánchez¹,

Rognli²,

Rostoks³

et al. 2022

Preprint

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Due to an increase in the consumption of food, feed, and fuel and to meet global food security needs for the rapidly growing human population, there is a necessity to obtain high-yielding crops that can adapt to future climate changes. Currently, the main feed source used for ruminant livestock production is forage grasses. In temperate climate zones, perennial grasses grown for feed are widely distributed and tend to suffer under unfavorable environmental conditions. Gene editing has been shown to be an effective tool for the development of abiotic stress-resistant plants. The highly versatile CRISPR-Cas system enables increasingly complex modifications in genomes while maintaining precision and low off-target frequency mutations. In this review, we provide an overview of forage grass species that have been subjected to gene editing. We offer a perspective view on the generation of plants resilient to abiotic stresses. Due to the broad factors contributing to these stresses the review focuses on drought, salt, heat, and cold stresses. The application of new genomic techniques (e.g., CRISPR-Cas) allows addressing several challenges caused by climate change and abiotic stresses for developing forage grass cultivars with improved adaptation to the future climatic conditions. Gene editing will contribute towards developing safe and sustainable food systems.

show abstract

Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops

Cited by 38 publications

References 565 publications

Mitigation of water scarcity with sustained growth of Rice by plant growth promoting bacteria

Mitigation of water scarcity with sustained growth of Rice by plant growth promoting bacteria

Improving abiotic stress tolerance of forage grasses – prospects of using genome editing

Improving Abiotic Stress Tolerance of Forage Grasses – Prospects of Using Genome Editing

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