Biotechnological applications of elastin-like polypeptides and the inverse transition cycle in the pharmaceutical industry

Fletcher, Emmanuella E.; Yan, Dandan; Kosiba, Anthony Adebayiga; Zhou, Yang; Shi, Haifeng

doi:10.1016/j.pep.2018.09.006

Cited by 60 publications

(30 citation statements)

References 95 publications

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“…ELRs are biomaterials of high interest because of their applications [18][19][20] in fields such as tissue engineering, regenerative medicine in general or controlled drug delivery. Moreover, the fact that they can be biosynthesized using the synthetic machinery in bacteria allows us to obtain polymers with a perfectly defined composition and amino acid sequence.…”

Section: Resultsmentioning

confidence: 99%

A novel lipase-catalyzed method for preparing ELR-based bioconjugates

Testera

Santos

Girotti

et al. 2019

International Journal of Biological Macromolecules

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Herein we present a novel one-pot method for the chemical modification of elastin-like recombinamers (ELRs) in a mild and efficient manner involving enzymatic catalysis with Candida antarctica lipase B. The introduction of different functionalities into such ELRs could open up new possibilities for the development of advanced biomaterials for regenerative medicine and, specifically, for controlled drug delivery given their additional ability to respond to stimuli other than pH or temperature, such as glucose concentration or electromagnetic radiation. Candida antarctica lipase B immobilized on a macroporous acrylic resin (Novozym 435) was used to enzymatically couple different aminated substrates to a recombinamer containing carboxylic groups along its amino acid chain by way of an amidation reaction. A preliminary study of the kinetics of this amidation in response to different reaction conditions, such as solvent, temperature or reagent ratio, was carried out using a phenylazobenzene derivative (azo-NH 2 ) as a model. The optimal amidation conditions were used to couple other amine reagents, such as phenylboronic acid (FB-NH 2 ) or polyethylene glycol (PEG-NH 2 ), thus allowing us to obtain photoresponsive, glucose-responsive or PEGylated ELRs that could potentially be useful as sensors in devices for controlled drug delivery.

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Section: Resultsmentioning

confidence: 99%

A novel lipase-catalyzed method for preparing ELR-based bioconjugates

Testera

Santos

Girotti

et al. 2019

International Journal of Biological Macromolecules

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“…In terms of purification, elastin-like polypeptides (ELPs) have been used as a purification tag on recombinantly expressed proteins using a protocol termed the inverse transition cycle (ITC) [13,77,78]. ELPs are artificial polypeptides, derived from the pentapeptide repeats, (VPGXG), found in human tropoelastin, where X can be any amino acid other than proline [13].…”

Section: Liquid-liquid Phase Separation: Segregation Purification Anmentioning

confidence: 99%

Phase separation: Bridging polymer physics and biology

Perry

2019

Current Opinion in Colloid & Interface Science

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Significant parallels exist between the phase separation behavior of polymers in solution and the types of biomolecular condensates, or 'membraneless organelles,' that are of increasing interest in living systems. Liquid-liquid phase separation allows for compartmentalization and the sequestration of materials, and can be harnessed as a sensitive strategy for responding to small changes in the environment. Here, I review many of the parallels and synergies between ongoing efforts to study and take advantage of phase separation in living vs. synthetic materials.

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“…They are soluble in cold water, but become insoluble and coacervate at above their transition temperature (T t ), which can be precisely controlled by managing the guest residue composition, molecular weight, and ELP concentration [13,14]. This stimuli-responsive coacervation property is the driving force behind the use of ELPs in the field of protein purification [15] and drug delivery technology [6,16]. Even though the preparation of thermoresponsive hydrogels of ELPs is of considerable interest, there has been limited success in the practical use of this application, because ELPs tend to macroscopically phase-separate from a solution above T t rather than form a gel as a continuous phase.…”

Section: Introductionmentioning

confidence: 99%

Rheology of Dispersions of High-Aspect-Ratio Nanofibers Assembled from Elastin-Like Double-Hydrophobic Polypeptides

Sugawara‐Narutaki

Yasunaga

Sugioka

et al. 2019

IJMS

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Elastin-like polypeptides (ELPs) are promising candidates for fabricating tissue-engineering scaffolds that mimic the extracellular environment of elastic tissues. We have developed a “double-hydrophobic” block ELP, GPG, inspired by non-uniform distribution of two different hydrophobic domains in natural elastin. GPG has a block sequence of (VGGVG)5-(VPGXG)25-(VGGVG)5 that self-assembles to form nanofibers in water. Functional derivatives of GPG with appended amino acid motifs can also form nanofibers, a display of the block sequence’s robust self-assembling properties. However, how the block length affects fiber formation has never been clarified. This study focuses on the synthesis and characterization of a novel ELP, GPPG, in which the central sequence (VPGVG)25 is repeated twice by a short linker sequence. The self-assembly behavior and the resultant nanostructures of GPG and GPPG were when compared through circular dichroism spectroscopy, atomic force microscopy, and transmission electron microscopy. Dynamic rheology measurements revealed that the nanofiber dispersions of both GPG and GPPG at an extremely low concentration (0.034 wt%) exhibited solid-like behavior with storage modulus G′ > loss modulus G” over wide range of angular frequencies, which was most probably due to the high aspect ratio of the nanofibers that leads to the flocculation of nanofibers in the dispersion.

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Biotechnological applications of elastin-like polypeptides and the inverse transition cycle in the pharmaceutical industry

Cited by 60 publications

References 95 publications

A novel lipase-catalyzed method for preparing ELR-based bioconjugates

A novel lipase-catalyzed method for preparing ELR-based bioconjugates

Phase separation: Bridging polymer physics and biology

Rheology of Dispersions of High-Aspect-Ratio Nanofibers Assembled from Elastin-Like Double-Hydrophobic Polypeptides

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