Biotechnological applications of microbial bioconversions

Perkins, Carrie; Siddiqui, Sana; Puri, Munish; Demain, Arnold L.

doi:10.3109/07388551.2015.1083943

Cited by 29 publications

(17 citation statements)

References 119 publications

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“…Because the upstream production of l ‐phe already has been thoroughly studied and the production of l ‐phe has reached a high level, improvement of the conversion efficiency of the whole‐cell system to produce t ‐CA from l ‐phe is very important. As noted previously, t ‐CA and its derivatives showed remarkable inhibitory effects on PAL . From studies with CdPAL1 from Cistanche deserticola , t ‐CA was reported to have the strongest inhibitory effects with a Ki of 8 μmol L –1 .…”

Section: Discussionmentioning

confidence: 53%

Development of a high‐efficiencytrans‐cinnamic acid bioproduction method by pH‐controlled separation technology

Zang

Xia

Zheng

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: Trans-cinnamic acid (t-CA) and its derivatives have been shown to be antioxidant and antibacterial, and can be used in food additives and flavors. However, a traditional t-CA production via chemical synthesis with a condensation reaction may not be suitable for its application in the pharmaceutical and food fields as it is not a 'green' route. This study investigated the bioconversion of L-phenylalanine (L-phe) to t-CA using engineered whole-cell Escherichia coli BL21(DE3) expressing phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) as a biocatalyst. To prevent product inhibition, an in situ reaction and separation of t-CA was performed using a pH-controlled biotransformation strategy. RESULTS:A coupling reaction and separation system based on pH adjustment was proposed for improving the bioproduction of t-CA. Using this process, 39.61 g L −1 t-CA was bioproduced from 40 g L −1 of L-phe in 8 h and a high purity (>98%) of t-CA product was achieved directly without additional purification. CONCLUSION:The bioprocess developed in this study is a highly efficient method for the bioproduction of t-CA and, therefore, offers a promising alternative route for t-CA production. Separation of t-CA from the reaction mixture by adjusting the pHThe reaction mixture was obtained after a 2 h bioconversion using 20 g L −1 of L-phe and 8.52 g L −1 DCW of cells at pH 9.0 and 55 ∘ C. After cells were removed by centrifugation, the supernatants were adjusted to different pH values by using HCl, and were allowed to stand at room temperature for 20 min, and then the acidified supernatants were centrifuged again for separating the t-CA precipitate. The concentrations of L-phe and t-CA in the acidified supernatants at different pH values were analyzed by HPLC.

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Section: Discussionmentioning

confidence: 53%

Development of a high‐efficiencytrans‐cinnamic acid bioproduction method by pH‐controlled separation technology

Zang

Xia

Zheng

et al. 2019

J of Chemical Tech & Biotech

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“…3 Imobilização é um termo geral que descreve as muitas formas de fixação, encapsulamento ou aprisionamento de células ou enzimas em um suporte sólido. Especificamente, a imobilização de células íntegras representa uma forma particular de adesão celular baseada na habilidade de certos microrganismos de se fixarem em suportes sólidos por meio de ligações naturais ou físico-químicas.…”

Section: Imobilização De Células íNtegrasunclassified

“…[1][2] Adicionalmente, outros atributos benéficos da biotransformação incluem versatilidade reacional, especificidade, redução no número de etapas no processo e alto rendimento. 3 Diferente da fermentação, na biotransformação o biocatalisador transforma molé-culas precursoras pré-formadas (naturais ou sintéticas) em limitado número de etapas. Além disto, o produto formado não é limitado pelo metabolismo do biocatalisador.…”

Section: Introductionunclassified

“…As células imobilizadas são preferidas em relação às células livres, uma vez que são mais convenientes de manusear e de separar do meio reacional, além de serem mais estáveis. 3 Cerca de dois terços das pesquisas em síntese orgânica por biotransformação envolvem a ação de enzimas hidrolíticas como as lipases, as proteases e as esterases. 4 Impulsionadas por sua versatilidade, as lipases apresentam destaque no cenário mundial de enzimas pois permitem a catálise de reações de hidrólise e de sín-tese, muitas vezes de forma quimio, regio e enantiosseletiva.…”

Section: Introductionunclassified

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Potencial catalítico de lipases ligadas ao micélio de fungos filamentosos em processos de biotransformação

2016

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“…Microbial transformation is the process by which fungus or bacteria are used to convert the substrate to a structurally related compound via one or a series of enzymatic reactions (Parshikov et al 2012;Perkins et al 2016). It is an excellent technique in modifying structure, enhancing efficacy and reducing toxicity of Traditional Chinese Medicine (TCM), due to its mild reaction conditions and convenient method (Cao et al 2015;Xie et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Biotransformation of 5-hydroxymethylfurfural into 2,5-dihydroxymethylfuran by Ganoderma sessile and toxicological assessment of both compounds

et al. 2020

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Biotransformation has the advantages of low cost and environmental protection and is a preferred method for production of compounds. At present, most 2,5-dihydroxymethylfuran (DHMF) is synthesized by chemical methods. In this study, 12.008 μg/mL DHMF was produced from 9.045 μg/mL 5-hydroxymethylfurfural (5-HMF) with a yield of 1.33 g/g using the crude enzymes from fungus Ganoderma sessile. To elucidate the toxic potential for both compounds, cytotoxicity tests and acute toxicity were evaluated respectively. 5-HMF induced weak cytotoxicity in HCT-8, A549 and SGC-7901 cells and DHMF exerted no cytotoxicity on HCT-8 while induced inhibition proliferation of A549 and SGC-7901 cells. The acute toxicity study showed no mortality happened in any group even at the single dose of 2000 mg/kg body weight. These results suggest it is feasible to convert 5-HMF to DHMF via crude enzymes from fungus G. sessile under mild condition, and that DHMF displays a potential effect of antitumor in vitro with little acute toxicity.

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Biotechnological applications of microbial bioconversions

Cited by 29 publications

References 119 publications

Development of a high‐efficiencytrans‐cinnamic acid bioproduction method by pH‐controlled separation technology

Development of a high‐efficiencytrans‐cinnamic acid bioproduction method by pH‐controlled separation technology

Potencial catalítico de lipases ligadas ao micélio de fungos filamentosos em processos de biotransformação

Biotransformation of 5-hydroxymethylfurfural into 2,5-dihydroxymethylfuran by Ganoderma sessile and toxicological assessment of both compounds

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