Biotechnological applications of Yarrowia lipolytica: Past, present and future

A two-stage fermentation process of erythritol production by yeast Y. lipolytica from molasses and glycerol

“…These results agree with other studies using Y. lipolytica grown on glycerol (Papanikolaou and Aggelis, 2002). The differences in fatty acids composition in the biomass of Y. lipolytica have been thoroughly studied by other groups (Liu et al, 2015). In agreement with the results, high contents of unsaturated fatty acids were reported before .…”

“…By introducing nutrient limitations, production of undesired by-products was reduced; this increased the selectivity of the process to 75.88%. Strikingly, the inhibitory effect on CA production was inconsistent with a previous study of nitrogen limitations that enhanced CA synthesis by Y. lipolytica (Liu et al, 2015). Nevertheless, citric acid production is not affected by nitrogen limitation in Y. lipolytica when hydrophobic materials are used as the carbon sources (Kamzolova et al, 2008).…”

Combining metabolic engineering and process optimization to improve production and secretion of fatty acids

“…These non-conventional microbes naturally accumulate high amounts of lipids ranging from 20 to 80% of the dry cell weight (DCW) (Sitepu et al, 2014) but often their metabolic knowledge and genetic tools are scarce. Yarrowia lipolytica is by far the most studied oleaginous yeast with a vast array of metabolic engineering techniques available (Beopoulos et al, 2009a;Liu et al, 2015a;Zhu and Jackson, 2015). Although Y. lipolytica present two main drawbacks since it accumulates limited amounts of lipids (Beopoulos et al, 2009a) and it cannot consume some preferred substrates such as lignocellulosic biomass or starch, it has been metabolically engineered to overcome these issues.…”

Isolation of Lipase-Producing Yeasts from Industrial Oily Residues in Different Culture Media

“…There were no significant differences between the mean cell concentration of medium 1 (2.04 g/L) and medium 2 (2.87 g/L) (p > 0.05), while medium 4 showed the lowest growth, at 0.93 g/L. Thus, the medium with the highest lipolytic activity (medium 4) was not the medium with the highest cell concentration (medium 3); possibly, this is due to the metabolism of the yeast, since productivity and lipase activity are affected by genetic and environmental factors (Liu et al, 2015). Another reason for this difference may be related to the growth kinetics of the microorganism and production of the lipase, classifying these processes as non-growthassociated products (Schmidell et al, 2004).…”