“…The Prussian blue histological staining method was utilized to detect intracellular iron concentrations [90,91]. RAW 264.7 cells in RPMI (1.5 mL) were plated into a cover glass at 3 × 10 5 cells/well in 6-well plates.…”
Section: Prussian Blue Staining For Intracellular Iron Detectionmentioning
Immune response to biomaterials, which is intimately related to their surface properties, can produce chronic inflammation and fibrosis, leading to implant failure. This study investigated the development of magnetic nanoparticles coated with silica and incorporating the anti-inflammatory drug naproxen, aimed at multifunctional biomedical applications. The synthesized nanoparticles were characterized using various techniques that confirmed the presence of magnetite and the formation of a silica-rich bioactive glass (BG) layer. In vitro studies demonstrated that the nanoparticles exhibited bioactive properties, forming an apatite surface layer when immersed in simulated body fluid, and biocompatibility with bone cells, with good viability and alkaline phosphatase activity. Naproxen, either free or encapsulated, reduced nitric oxide production, an inflammatory marker, while the BG coating alone did not show anti-inflammatory effects in this study. Overall, the magnetic nanoparticles coated with BG and naproxen showed promise for biomedical applications, especially anti-inflammatory activity in macrophages and in the bone field, due to their biocompatibility, bioactivity, and osteogenic potential.
“…The Prussian blue histological staining method was utilized to detect intracellular iron concentrations [90,91]. RAW 264.7 cells in RPMI (1.5 mL) were plated into a cover glass at 3 × 10 5 cells/well in 6-well plates.…”
Section: Prussian Blue Staining For Intracellular Iron Detectionmentioning
Immune response to biomaterials, which is intimately related to their surface properties, can produce chronic inflammation and fibrosis, leading to implant failure. This study investigated the development of magnetic nanoparticles coated with silica and incorporating the anti-inflammatory drug naproxen, aimed at multifunctional biomedical applications. The synthesized nanoparticles were characterized using various techniques that confirmed the presence of magnetite and the formation of a silica-rich bioactive glass (BG) layer. In vitro studies demonstrated that the nanoparticles exhibited bioactive properties, forming an apatite surface layer when immersed in simulated body fluid, and biocompatibility with bone cells, with good viability and alkaline phosphatase activity. Naproxen, either free or encapsulated, reduced nitric oxide production, an inflammatory marker, while the BG coating alone did not show anti-inflammatory effects in this study. Overall, the magnetic nanoparticles coated with BG and naproxen showed promise for biomedical applications, especially anti-inflammatory activity in macrophages and in the bone field, due to their biocompatibility, bioactivity, and osteogenic potential.
“…When the exposure concentration of nano-magnetite particles is greater than 50 µg/ml, it will produce greater cytotoxicity in the form of reduced activity, and increase ROS, reduce cell membrane potential and reduce the rate of cell apoptosis [ 30 ]. Therefore, as long as the specific parameters of IONPs are controlled, they will not have adverse effects on cell viability, metabolic activity, oxidative stress, proliferation and differentiation [ 9 , 35 , 36 ]. Fig.…”
Section: Fate Of Ionps In Cellsmentioning
confidence: 99%
“…It also promoted the down-regulation of GPX4 [ 81 ] and xCT [ 82 ], and lipid peroxide began to accumulate. Apoptosis of caspase-8 activated cells after short time exposure to AC magnetic field [ 36 ]. Zhou et al [ 83 ] showed that gold-plated iron oxide particles(Fe 3 O 4 @Au SPs) can induce non-cytotoxicity and gene expression in tumor cells, which are mainly involved in cell proliferation, differentiation and apoptosis.…”
At present, iron oxide nanoparticles (IONPs) are widely used in the biomedical field. They have unique advantages in targeted drug delivery, imaging and disease treatment. However, there are many things to pay attention to. In this paper, we reviewed the fate of IONPs in different cells and the influence on the production, separation, delivery and treatment of extracellular vesicles. It aims to provide cutting-edge knowledge related to iron oxide nanoparticles. Only by ensuring the safety and effectiveness of IONPs can their application in biomedical research and clinic be further improved.
“…In addition, PTT reshaped the tumor extracellular matrix and reduced the amount of CAF in the TME ( Tan et al, 2019 ; Zheng et al, 2020b ). The oxidized nanoparticles developed by Ferraz had the characteristics of fast and efficient cells internalization and low cytotoxicity ( Ferraz et al, 2020 ). Ferric chloride and ferric sulfate were coprecipitated in sodium hydroxide solution and coated with trisodium citrate solution at 90°C for 30 min.…”
Section: Nanomaterials For Regulating Cancer-associated Fibroblast To...mentioning
Despite advances in cancer treatment, metastatic cancer is still the main cause of death in cancer patients. At present, the treatment of metastatic cancer is limited to palliative care. The abscopal effect is a rare phenomenon in which shrinkage of metastatic tumors occurs simultaneously with the shrinkage of a tumor receiving localized treatment, such as local radiotherapy or immunotherapy. Immunotherapy shows promise for cancer treatment, but it also leads to consequences such as low responsiveness and immune-related adverse events. As a promising target-based approach, intravenous or intratumoral injection of nanomaterials provides new opportunities for improving cancer immunotherapy. Chemically modified nanomaterials may be able to trigger the abscopal effect by regulating immune cells. This review discusses the use of nanomaterials in killing metastatic tumor cells through the regulation of immune cells and the prospects of such nanomaterials for clinical use.
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