Biotechnological Approaches for Ex Situ Conservation of Medicinal Plants

Micropropagation is widely used for vegetative propagation of many different ornamental and medicinal plant species under aseptic conditions. This technology has widespread practical applications irrespective of seasonal and time-space constraints. The present study aimed to design in vitro propagation using 1 8 different combinations of BAP, NAA, TDZ, and GA3 supplemented with commercial tea sugar at 30 g l-1 for micropropagation using leaf segments close to petiole of Kalanchoe blossfeldiana as explants and to find its efficiency for viable cultures. The results showed clear discrimination between regeneration on TDZ and BAP-supplemented regeneration cultures. Thus, both BAP-NAA and TDZ-NAA combinations were found suitable for micropropagation. the source medium affected the shoot regeneration and the rooting ability, irrespective of the culture of any shoot regeneration medium. These plantlets were rooted in 0.01-0.05 mg l-1 IBA. The study showed successful rooting under in vitro conditions using 1 ½ or 1×MS medium with 0.5 mg L-1 IBA. These rooted plantlets were then transferred to MS medium with 0.2 mg L-1 gibberellic acid and without BAP or with 0.1, 0.2, 0.5 mg l-1 TDZ and 0.1 mg l-1 NAA or without 0.1 mg L-1 NAA for adaptation. The results of the study are of great importance for K. blossfeldiana plant breeding studies and growers.

show abstract

Preserving Nature's Balance in the Intelligent Systems Era

Garole,

Reddy,

Lippert

2024

Advances in Mechatronics and Mechanical Engineering

View full text Add to dashboard Cite

The proposed chapter provides a thorough examination of related ideas, beginning with a basic knowledge of ecological systems and the significance of biodiversity, explores the patterns and processes of biodiversity, including the measurements and methods for quantifying it along with the variables, like habitat heterogeneity and latitude, that affect its distribution. The paper then explores the patterns and processes of biodiversity, including the measurements and methods for quantifying it along with the variables, like habitat heterogeneity and latitude, that affect its distribution.. The study also outlines important risks to biodiversity, highlighting the significant contribution of human activity to biodiversity loss. The study examines potential future paths for biodiversity conservation and research, taking into account new developments in technology and fashion while recognizing the continuous difficulties in maintaining biodiversity in the face of global change.

show abstract

Biotechnological Approaches for Ex Situ Conservation of Medicinal Plants

Cited by 3 publications

References 261 publications

In vitro culture of Atraphaxis frutescens (L.) K. Koch: effects of D-mannitol and pH on a propagation coefficient, secondary-metabolite levels, and antiradical activity

In vitro culture of Atraphaxis frutescens (L.) K. Koch: effects of D-mannitol and pH on a propagation coefficient, secondary-metabolite levels, and antiradical activity

The effect of different cytokinin and auxin source on micropropagation of Kalanchoe blossfeldiana

Preserving Nature's Balance in the Intelligent Systems Era

Contact Info

Product

Resources

About