Biotechnological Approaches to Study Plant Responses to Stress

Pérez-Clemente, Rosa M.; Vives‐Peris, Vicente; Zandalinas, Sara I.; López-Climent, María F.; Muñoz, Valeria; Gómez-Cádenas, Aurelio

doi:10.1155/2013/654120

Cited by 134 publications

(68 citation statements)

References 86 publications

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“…The data collected from the completed sequencing genome projects provide valuable information about genes to be analyzed (Pérez-Clemente et al 2013 ). The availability of this plant genome sequence information currently facilitates studying the function of genes on a genome-wide level (Feuillet et al 2010 ;Chain et al 2009 ).…”

Section: Advances In Plant Genomic Technologiesmentioning

confidence: 99%

Towards Understanding Abiotic Stress Signaling in Plants: Convergence of Genomic, Transcriptomic, Proteomic, and Metabolomic Approaches

Soni

Nutan

Soda

et al. 2015

Elucidation of Abiotic Stress Signaling in Plants

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All aspects of a plant's life-beginning with the seed germination and ending with the seed formation-are adversely affected by different abiotic stresses such as salinity, fl ood, drought, heat, cold, etc. Being sessile, plants have developed excellent mechanisms of stress perception and signal transduction. Multiple, complex, and dynamically intertwined interactions among nucleic acids, proteins, and metabolites determine the phenotype and fi nal response of plants towards environmental stresses. In response to these stresses, a multitude of processes are activated which enable the plants to cope with these stresses up to a certain extent. These include alteration of expression of stress-responsive genes, production of stress proteins, alteration of ion transport, activation of various antioxidant systems, and compatible solute accumulation. Our knowledge of abiotic stress signaling has grown in leaps and bounds since the emergence and developments in the omics technologies. Genome-scale studies at transcript, protein, and metabolite levels provide information about dynamic changes taking place at these functional levels. For full understanding of signaling networks, it is essentially important to integrate all these aspects. This approach is of remarkable applicability when the aim is to understand how plants react to abiotic stresses. In order to understand molecular basis of stress tolerance along with signaling network under unfavorable environmental situations, recent progress on systematic use of omics technologies including genomics, transcriptomics, proteomics, and metabolomics has been summarized in this chapter.

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Section: Advances In Plant Genomic Technologiesmentioning

confidence: 99%

Towards Understanding Abiotic Stress Signaling in Plants: Convergence of Genomic, Transcriptomic, Proteomic, and Metabolomic Approaches

Soni

Nutan

Soda

et al. 2015

Elucidation of Abiotic Stress Signaling in Plants

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show abstract

“…232 Em uma busca rápida nas bases de dados de periódicos é possível encontrar nos últimos dezessete anos mais de 100 artigos de revisão na área, em diversas aplicações. [233][234][235][236][237][238][239] Dentre estas revisões, destacam-se algumas com enfoque em estudos de alterações ambientais 71,77 e genéticas, [240][241][242] resposta ao estresse, [243][244][245] saúde e tratamento de doenças, [246][247][248] ecologia e evolução, 63,76 agricultura, 249,250 bem como trabalhos dedicados à metabolômica com uso de compostos isotópicos, 251,252 que auxiliam na elucidação de rotas e melhoram a identificação e quantificação de metabólitos. Também são frequentemente encontrados estudos envolvendo mais de uma abordagem ômica, como proteômica e transcriptômica.…”

Section: Plantasunclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

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METABOLOMICS: DEFINITIONS, STATE-OF-THE-ART AND REPRESENTATIVE APPLICATIONS.Metabolomics is an emerging and promising omics approach used to understand biological mechanisms. By untargeted and targeted metabolomics analyses, metabolites are determined in biological samples (fluids, cells, tissues, etc.) by comparison of control groups with altered groups, undergoing different therapies, submitted to differing stress levels, dietary modulation, or promoted by a disease, or specific condition, etc., using sophisticated analytical techniques, and advanced data treatment and statistical analyses. In this review, the concepts involved in metabolomics studies were presented, describing in details all steps involved in the metabolomics workflow, for untargeted and targeted strategies. Finally, the potential of metabolomics is illustrated by applications in representative areas: clinical, environmental, food and nutrition, forensic toxicology, microbiology, parasitology, plants, and sports. Relevant reviews were compiled to characterize each of these areas, and a corresponding application of untargeted and targeted metabolomics were described.Keywords: metabolomics; untargeted metabolomics; targeted metabolomics; metabolomics workflow. INTRODUÇÃOAs ciências ômicas buscam o entendimento do funcionamento celular dos organismos e suas alterações biológicas. Fazem parte deste conjunto de ciências, a genômica (estudo da alteração dos genes), a transcriptômica (estudo das alterações dos transcritos), a proteômica (estudo das alterações das proteínas), e a metabolômica (estudo das alterações dos metabólitos). Metabólitos são produtos intermediários ou finais do metabolismo em uma amostra biológica.1 O conjunto de todos os metabólitos de baixa massa molecular (até 1500 Da), presentes ou alterados em um sistema biológico, é chamado de metaboloma (do inglês, metabolome).2 A pesquisa relacionada a metabólitos vem sendo desenvolvida há décadas, mas em 1999, Nicholson et al. definiram a metabonômica (do inglês, metabonomics), como sendo a medida quantitativa da resposta metabólica de um sistema biológico após estímulos fisiopatológicos ou modificações genéticas.3 Já o termo metabolômica (do inglês, metabolomics) foi introduzido em 2001, por Oliver Fiehn, como sendo a análise abrangente e quantitativa do metaboloma de um sistema biológico. 4 Além disso, a literatura nos apresenta outras denominações envolvendo esse campo da ciência, tais como: perfil metabólico (do inglês, metabolic profiling), descrito como sendo a análise de metabólitos previamente selecionados de rotas bioquímicas específicas, 5 a impressão digital metabólica (do inglês, metabolic fingerprinting), que é definida como sendo "uma classificação de amostras de acordo com sua origem ou sua relevância biológica", 3 e, por último, análise footprinting (ainda sem tradução para o português), para se referir aos metabólitos excretados por uma célula em condições controladas. Observa-se, portanto, uma divergência em relação às terminologias aplicadas às análises metabolômic...

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“…The signaling pathway of any abiotic stress involves certain key steps such as signal perception, transduction, responsiveness, combined with activation of physiological, and metabolic reactions (Pérez-Clemente et al, 2013;Liu et al, 2014). In this process, plant cells first perceive stress stimulus through sensors or receptors localized mostly at the cell membrane.…”

Section: Growth Assessmentmentioning

confidence: 99%

Effects of in vitro Drought Stress on Growth, Proline Accumulation and Antioxidant Defense in Sugarcane

Oliveira¹,

Cardoso²,

Oliveira³

et al. 2018

JAS

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Drought is the most limiting environmental factor to crop productivity and presents a great variability in the degree of tolerance among and within species, among varieties. The aim of this study was to characterize sugarcane accessions regarding tolerance to water stress during in vitro cultivation based on changes in biometric, physiological and biochemical characteristics, within species and among species, to support future breeding programs. Adventitious shoots of five sugarcane accessions: Saccharum robustum, Saccharum spontaneum and Saccharum officinarum species, cultivated in Murashige and Skoog medium supplemented with 2% sucrose and 4 g/l Phytagel were used in five water potentials, 0, -0.3, -0.6, -0.9, -1.2 MPa, induced by mannitol. Survival, length of shoots and roots, number of shoots and roots, biomass, proline content in leaves and activity of antioxidant enzymes were analyzed. There is difference among species, and also, within the same sugarcane species when submitted to in vitro drought stress, and S. officinarum was shown to be the most tolerant. Proline can be used as a biochemical indicator of response to drought in sugarcane accessions and its accumulation was intensified in S. robustum and S. spontaneum accessions. Catalase activity remained unchanged with increased drought in sugarcane accessions evaluated.

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Biotechnological Approaches to Study Plant Responses to Stress

Cited by 134 publications

References 86 publications

Towards Understanding Abiotic Stress Signaling in Plants: Convergence of Genomic, Transcriptomic, Proteomic, and Metabolomic Approaches

Towards Understanding Abiotic Stress Signaling in Plants: Convergence of Genomic, Transcriptomic, Proteomic, and Metabolomic Approaches

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Effects of in vitro Drought Stress on Growth, Proline Accumulation and Antioxidant Defense in Sugarcane

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