Biotechnological Approaches Towards Improvement of Medicinal Plants

Dioscorea bulbifera L. containing the pharmaceutically important compound, diosgenin, was regenerated in vitro through nodal segments on supplemented Murashige and Skoog medium (MS). Diosgenin was at 12 mg g(-1)dry wt in 12-week-old plantlets raised on MS with various growth hormones. Random amplified polymorphic DNA (RAPD) analysis showed genetic fidelity of regenerants. Encapsulation of shoot tips in 3% (w/v) calcium alginate for storage and germplasm exchange was achieved.

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“…Pande et al (2002) reported IAA + Kn combination for high regeneration in Ammi majus. In (Narula et al 2004). …”

Section: Multiplication Through Nodal Segmentsmentioning

confidence: 94%

“…One of these herbs is Dioscorea deltoidea, the major source of diosgenin. Besides the conventional methods of propagation and conservation, in vitro culture techniques can help salvage the situation (Narula et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Genetic fidelity of in vitro regenerants, encapsulation of shoot tips and high diosgenin content in Dioscorea bulbifera L., a potential alternative source of diosgenin

2007

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“…were confirmed as endophytes, these were further used as a source of biotic elicitor. Many workers have demonstrated that subjecting the cultures of medicinal plants to biotic stresses can be crucial in increasing the yield of secondary metabolites (Saba et al 2000;Narula et al 2004). C. inophyllum is a slow-growing, woody tree species and its suspension cultures also responded slowly to the elicitor treatment.…”

Section: Discussionmentioning

confidence: 99%

Influence of endophytic fungal elicitation on production of inophyllum in suspension cultures of Calophyllum inophyllum L.

Pawar

Yadav

Shouche

et al. 2011

Plant Cell Tiss Organ Cult

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The influence of dried cell powder and culture filtrates of endophytic fungi on production of inophyllum in cell suspension cultures of leaf-and stem-derived callus of Calophyllum inophyllum was investigated. Two fungi, Nigrospora sphaerica and Phoma spp., endophytic to C. inophyllum, were isolated from leaf tissues, and were identified by both 18S rRNA gene amplification and sequencing. Elicitation of suspension cultures of both callus types of C. inophyllum with dried cell powder and culture filtrates of both fungi consistently elicited production of inophyllum A, B, C, and P. In comparison to stemderived callus, suspension cultures of leaf-derived callus enhanced production of most inophyllum. Of the four inophyllum studied, the highest production of inophyllum A, C, and P was achieved in elicited suspension cultures of leaf-derived callus. Suspension cultures of stem-derived callus enhanced production only of inophyllum B. When suspension cultures of leaf-derived callus were elicited with 40 mg dried cell powder of Phoma spp., a level of 751-fold (6.84 mg/100 g elicited biomass) of inophyllum A was produced, compared to control. Whereas, a level of 414-fold (6.22 mg/100 g elicited biomass) of inophyllum B was produced when suspension cultures of stem-derived callus were elicited with 20 mg dried cell powder of N. sphaerica. When compared to control, a 10% culture filtrate of N. sphaerica in suspension cultures of leafderived callus elicited inophyllum C and P production by 928-fold (7.43 mg/100 g elicited biomass) and 750-fold (1.5 mg/100 g elicited biomass), respectively.

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“…In the present study, effect of CaCl 2 on production of inophyllums and biomass growth was also studied. Many workers have demonstrated that subjecting the cultures of medicinal plants to abiotic stresses can be crucial in increasing the yield of secondary metabolites (Saba et al 2000;Narula et al 2004). Elicitation of plant cells in culture represents one of the useful biotechnological tools to improve the production of valuable secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

Influence of abiotic elicitation on production of dipyranocoumarins in suspension cultures of Calophyllum inophyllum L.

Pawar

Thengane

2011

Acta Physiol Plant

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Effects of elicitation with heavy metals such as copper, cadmium, chromium (abiotic elicitation) and supplementation of CaCl 2 on production of dipyranocoumarins (inophyllums) in suspension cultures of leaf and stem callus of Calophyllum inophyllum were studied. The optimum timing for elicitor introduction was found to be the 10th day after initiating the suspension cultures. Cadmium as abiotic elicitor in suspension cultures of stem callus was found best to elicit maximum production of inophyllums A, C, and calophyllolide while cadmium in suspension cultures of leaf callus was found best for eliciting maximum production of inophyllums B and P. Inophyllum D was the only dipyranocoumarin whose highest production was achieved when 1.0 mM chromium was used as abiotic elicitor in suspension cultures of stem callus. Out of the three abiotic elicitors used, none could result biomass growth. Only incorporation of CaCl 2 in suspension cultures resulted biomass growth. A maximum of 35.26-fold biomass growth was achieved when suspension cultures of stem callus were incorporated with 2.0 mM CaCl 2 . CaCl 2 was noted to have no positive influence on production of most of the dipyranocoumarins under study.

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Biotechnological Approaches Towards Improvement of Medicinal Plants

Cited by 12 publications

References 176 publications

Genetic fidelity of in vitro regenerants, encapsulation of shoot tips and high diosgenin content in Dioscorea bulbifera L., a potential alternative source of diosgenin

Genetic fidelity of in vitro regenerants, encapsulation of shoot tips and high diosgenin content in Dioscorea bulbifera L., a potential alternative source of diosgenin

Influence of endophytic fungal elicitation on production of inophyllum in suspension cultures of Calophyllum inophyllum L.

Influence of abiotic elicitation on production of dipyranocoumarins in suspension cultures of Calophyllum inophyllum L.

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